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Picogreen dsdna assay kit

Manufactured by Roche
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The PicoGreen™ dsDNA Assay Kit is a fluorescence-based solution used to quantify double-stranded DNA (dsDNA) in biological samples. The kit contains a proprietary dye that binds to dsDNA, allowing for sensitive and accurate measurement of DNA concentration.

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Lab products found in correlation

Qiaquick pcr purification kit, by Sage Science (2 mentions) Hiseq4000 v2, by Illumina (2 mentions)

Spelling variants of this product

Picogreen assay (2 citations) Quant-iT PicoGreen dsDNA Assay kit (2 citations)

2 protocols using picogreen dsdna assay kit

1

Illumina HiSeq4000 DNA Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracted DNA was quantified with PicoGreen™ dsDNA Assay Kit, and 1 ng of input, or maximum 3.5 μl, gDNA was used in a 1:10 miniaturized Kapa HyperPlus protocol. Per sample libraries were quantified and pooled at equal nanomolar concentration. The pooled library was cleaned with the QIAquick PCR Purification Kit and size selected for fragments between 300 and 700 bp on the Sage Science PippinHT. The pooled library was sequenced as a paired-end 150-cycle run on an Illumina HiSeq4000 v2 at the UCSD IGM Genomics Center.
Mills R.H., Dulai P.S., Vázquez-Baeza Y., Sauceda C., Daniel N., Gerner R.R., Batachari L.E., Ochoa M.M., Zhu Q., Weldon K., Humphrey G., Carrillo-Terrazas M., Goldasich L.D., Bryant M., Raffatellu M., Quinn R.A., Gewirtz A.T., Chassaing B., Chu H., Sandborn W.J., Dorrestein P.C., Knight R, & Gonzalez D.J. (2022). Multi-omics analyses of the Ulcerative Colitis gut microbiome link Bacteroides vulgatus proteases with disease severity. Nature microbiology, 7(2), 262-276.
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2

Illumina HiSeq4000 DNA Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracted DNA was quantified with PicoGreen™ dsDNA Assay Kit, and 1 ng of input, or maximum 3.5 μl, gDNA was used in a 1:10 miniaturized Kapa HyperPlus protocol. Per sample libraries were quantified and pooled at equal nanomolar concentration. The pooled library was cleaned with the QIAquick PCR Purification Kit and size selected for fragments between 300 and 700 bp on the Sage Science PippinHT. The pooled library was sequenced as a paired-end 150-cycle run on an Illumina HiSeq4000 v2 at the UCSD IGM Genomics Center.
Mills R.H., Dulai P.S., Vázquez-Baeza Y., Sauceda C., Daniel N., Gerner R.R., Batachari L.E., Ochoa M.M., Zhu Q., Weldon K., Humphrey G., Carrillo-Terrazas M., Goldasich L.D., Bryant M., Raffatellu M., Quinn R.A., Gewirtz A.T., Chassaing B., Chu H., Sandborn W.J., Dorrestein P.C., Knight R, & Gonzalez D.J. (2022). Multi-omics analyses of the Ulcerative Colitis gut microbiome link Bacteroides vulgatus proteases with disease severity. Nature microbiology, 7(2), 262-276.
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