Rock1 sirna si rock1

Manufactured by GenePharma

Sourced in China

ROCK1 siRNA (si-ROCK1) is a laboratory reagent designed to specifically target and silence the expression of the ROCK1 gene. ROCK1 is a serine/threonine protein kinase that plays a role in various cellular processes. The si-ROCK1 product can be used in research applications to investigate the function of the ROCK1 gene and its downstream effects.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Jetprime reagent, by Thermo Fisher Scientific (1 mentions) Mir 335 5p mimics, by GenePharma (1 mentions)

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SiRNAs (565 citations) Si-ROCK1 (4 citations)

2 protocols using rock1 sirna si rock1

Osteosarcoma Cell Lines Transfection

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The human OC cell lines U2OS, MG-63, HOS and human normal osteoblast cell line hFOB1.19 were used for this experiment. All the cell lines came from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were seeded in DMEM or RPMI-1640 medium containing 10% fetal bovine serum (FBS) and cultured at 37°C with 5% CO₂.
The miR-202-5p mimic and inhibitor, ROCK1 siRNA (si-ROCK1) were obtained from GenePharma Co., Ltd. (Shanghai, China). Then they were transferred into HOS cells using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) based on the manufacturer's protocols.

Li C., Ma D., Yang J., Lin X, & Chen B. (2018). miR-202-5p inhibits the migration and invasion of osteosarcoma cells by targeting ROCK1. Oncology Letters, 16(1), 829-834.

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Transfection of miR-335-5p and ROCK1 siRNA

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A549 and SPC-A1 cells were seeded in 6-well plates. When cells had reached 40–60% confluence, we performed transfection in accordance with the manufacturer’s instructions using jetprime reagent (Invitrogen). Cells were collected at 48–72 h after transfection for further experiments. MiR-335-5p mimics, ROCK1 siRNA (Si-ROCK1) and corresponding control were purchased from GenePharma company (Suzhou, China). The target siRNA sequences were as follows: ROCK1si-1 5′-GCAGAUGAAACAGGAAAUA-3′, ROCK1 si-2 5′-GGCAGAGGAAGAAUAUAAA-3′.

Du W., Tang H., Lei Z., Zhu J., Zeng Y., Liu Z, & Huang J.A. (2019). miR-335-5p inhibits TGF-β1-induced epithelial–mesenchymal transition in non-small cell lung cancer via ROCK1. Respiratory Research, 20, 225.

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