The level of intracellular ROS for each treatment group was measured using a fluorescent probe, 2′, 7′ -dichlorodihydrofluorescein diacetate (DCF-DA; Invitrogen, Carlsbad, CA, USA), as previously described [45 (link)]. Cells were plated at a density of 1 × 106 cells/mL and treated with GSH for 24 hr. After GSH pretreatment, H/R was performed. Then, bEND.3 cells were treated with 5 μM DCF-DA for 30 min at 37°C. After washing with PBS, fluorescence was measured with a microscope (Nikon TS100-F ECLIPSE) equipped with a CCD camera (Hamamatsu Photonics) [43 (link)].
Ts100 f eclipse
Manufactured by Hamamatsu Photonics
Sourced in Japan
The TS100-F ECLIPSE is a laboratory microscope designed by Hamamatsu Photonics. It features a binocular viewing head, a mechanical stage, and a built-in illumination system. The microscope is capable of brightfield and phase contrast observation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using ts100 f eclipse
1
Measuring Intracellular Reactive Oxygen Species
2
Intracellular ROS Measurement Using DCF-DA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The level of the intracellular ROS in all groups was measured using a fluorescent probe, 2', 7'-dichlorodihydrofluorescein diacetate (DCF-DA; Invitrogen, CA, USA) as previously described [29 (link)]. The 1×106 cells/ml were seeded in the plate and were treated with H2O2 or/and GSH for 24 h. Then, b END.3 cells were treated with 5 µM DCF-DA for 30 min at 37℃, and after washing with PBS, the fluorescence was measured in a microscope (Nikon TS100-F ECLIPSE) equipped with a CCD camera (Hamamatsu Photonics, Shizuoka, Japan) [30 (link)].
3
Measuring Intracellular ROS Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The level of intracellular ROS in each treatment group was measured using a fluorescent probe, 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA; Invitrogen, CA, USA), as previously described [59 (link)]. Cells were plated at a density of 1 × 106 cells/mL and treated with melatonin for 24 h. After melatonin pretreatment, OGD injury and reperfusion were conducted. Then, bEND.3 cells were treated with 5 μM DCF-DA for 30 min at 37°C. After washing with PBS, fluorescence was measured with a microscope (Nikon TS100-F ECLIPSE) equipped with a CCD camera (Hamamatsu Photonics) [54 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!