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Fluorometric lactate assay kit

Manufactured by Cell Biolabs
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Sourced in United States

The Fluorometric Lactate Assay Kit is a fluorescence-based kit designed to quantify lactate levels in biological samples. The kit uses an enzyme-coupled reaction to produce a fluorescent signal that is proportional to the amount of lactate present. The assay is sensitive, specific, and can be used with a variety of sample types, including cell culture media, serum, and tissue homogenates.

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Lab products found in correlation

Sodium pyruvate, by Biowest (1 mentions) Carnitine, by Merck Group (1 mentions) Xf24 extracellular flux analyzer, by Agilent Technologies (1 mentions) Seahorse xf base medium, by Merck Group (1 mentions) Xf cell mito stress kit, by Agilent Technologies (1 mentions) Filtermax f5, by Molecular Devices (1 mentions) L glutamine, by Biowest (1 mentions) Seahorse xf96 extracellular flux analyzer, by Agilent Technologies (1 mentions) Glucose assay kit, by Cell Biolabs (1 mentions) Human il 6 quantikine elisa, by R&D Systems (1 mentions) Pierce ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions) Il 1 beta human uncoated elisa kit, by Thermo Fisher Scientific (1 mentions) Human il 18 elisa kit, by MBL Life Science (1 mentions)

2 protocols using fluorometric lactate assay kit

1

Comprehensive Metabolic Profiling of Immune Cells

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Cellular oxygen consumption (OCR) and extracellular acidification rates (ECAR) were determined in Seahorse XF Base Medium supplemented with 25 mM glucose (Sigma-Aldrich), 2 mM L-glutamine and 1 mM sodium pyruvate (both from BioWest) using the XF cell Mito Stress Kit (SeaHorse Bioscience), in an XF24 Extracellular Flux Analyzer (SeaHorse Bioscience; Agilent Technologies). Fatty acid oxidation (FAO) was determined in Krebs-Henseleit buffer (KHB) supplemented with 0.5 mM carnitine (Sigma-Aldrich) and 2.5 mM glucose, using palmitate as substrate, in the Agilent Seahorse XF96 Extracellular Flux Analyzer.
Lactate levels were determined enzymatically in extracts from TCTRL, TACT and TACT + PD1 cells after 48 h stimulation, using a fluorometric lactate assay kit (Cell Biolabs) according to supplier’s protocol; fluorescence was quantified in a Filter Max F5 microplate reader (Molecular Devices) at 530/590 nm excitation/emission. A lactate standard curve was generated in all assays and used to extrapolate relative fluorescent units (RFU) measured in the samples.
Ogando J., Sáez M.E., Santos J., Nuevo-Tapioles C., Gut M., Esteve-Codina A., Heath S., González-Pérez A., Cuezva J.M., Lacalle R.A, & Mañes S. (2019). PD-1 signaling affects cristae morphology and leads to mitochondrial dysfunction in human CD8+ T lymphocytes. Journal for Immunotherapy of Cancer, 7, 151.
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2

Cytokine and Metabolic Profiling of CF Cultures

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Conditioned supernatants of human CF cultures were snapfrozen at -80 °C and assessed by colorimetric/fluorometric assay or ELISA using the following kits as instructed by the manufacturer: Pierce® LDH Cytotoxicity Assay Kit and Human IL-1beta Uncoated ELISA Kit (both from Thermo Fisher), Glucose Assay Kit and Fluorometric Lactate Assay Kit (both from Cell Biolabs, San Diego, CA, USA), Human IL-6 Quantikine ELISA (R&D Systems, Wiesbaden, Germany), Human IL-18 ELISA Kit (MBL International, via Biozol, Eching, Germany).
Mann C., van Alst C., Gorressen S., Nega R., Dobrev D., Grandoch M, & Fender A.C. (2024). Ischemia does not provoke the full immune training repertoire in human cardiac fibroblasts. Naunyn-Schmiedeberg's archives of pharmacology.
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