The human monocytic cell line THP-1 (ATCC,TIB- 202) was maintained in RPMI with 2% GlutaMax (Gibco), 10% fetal bovine serum (FBS, Gibco), penicillin (100 units/mL), and streptomycin (100 μg/mL). The experiments were conducted using LNCaP (ATCC) as an androgen-sensitive prostate cancer cell line model and C4–2 cells (gift from Dr. Wade Bushman) as an androgen-independent prostate cancer model. Both LNCaP and C4–2 were maintained in RPMI with 10% fetal bovine serum (FBS, Gibco), penicillin (100 units/mL), and streptomycin (100 μg/mL). Human umbilical vein endothelial cells (HUVEC, Lonza) were cultured in Endothelial Cell Growth Media kits (EGM-2 Bulletkit, Lonza). Primary neonatal normal human dermal fibroblasts (NHDF, ATCC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, containing 1000 mg/L glucose, L-glutamine, and sodium bicarbonate), 10% FBS, penicillin (100 units/mL), and streptomycin (100 μg/mL). All cells were maintained in a 37 °C incubator with 5% carbon dioxide. Cells were used at the following passage numbers: THP-1 P3–11, HUVEC P3-P7, NHDF P7-P13. All cell lines tested negative for mycoplasma.
Normal human dermal fibroblasts (nhdf)
Manufactured by American Type Culture Collection
Sourced in United States
The NHDF is a laboratory equipment product offered by the American Type Culture Collection (ATCC). It is a primary human dermal fibroblast cell line derived from normal human skin. The core function of the NHDF is to provide a reliable and consistent cell model for in vitro research applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Lncap, by American Type Culture Collection (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
96 well plate, by Corning (3 mentions)
Snb 19, by Leibniz Institute DSMZ (3 mentions)
Caco 2, by American Type Culture Collection (3 mentions)
Wm115, by American Type Culture Collection (2 mentions)
Penicillin streptomycin, by Solarbio (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Ssc dc58 ap camera, by Olympus (2 mentions)
Minimum essential medium (mem), by Merck Group (2 mentions)
Penicillin g, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Lncap, by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by Lonza (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (1 mentions)
30 protocols using normal human dermal fibroblasts (nhdf)
1
Comparative Cell Culture Protocols for Prostate Cancer and Non-Cancer Cell Lines
2
Established Cell Lines and Claspin Mutants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
293T, HeLa, NHDF, HCT116 and U2OS were obtained from ATCC. Claspin flox /- Mouse Embryonic Fibroblasts (MEFs) were established from E12.5 embryos (Yang et al., 2016 (link)). Claspin flox /- MEFs stably expressing the wild-type or APDE/A mutant Claspin were established by infecting recombinant retroviruses expressing these cDNAs (Yang et al., 2016 (link)). Cells were grown in Dulbecco’s modified Eagle’s medium (high glucose) supplemented with 15% fetal bovine serum (NICHIREI), 2 mM L-glutamine, 1% sodium pyruvate, 100 U/ml penicillin and 100 μg/ml streptomycin in a humidified atmosphere of 5% CO2, 95% air at 37°C. HCT-15-Luc#1, NCI-H1975-Luc, NUGC-3 were grown in RPMI supplemented with 10% fetal bovine serum (Gibco); HL-60 were grown in RPMI supplemented with 55 μM β- 2-mercaptoethanol and 10% fetal bovine serum (Gibco); 5K-BR-3-Luc were grown in McCoy’s 5A supplemented with 10% fetal bovine serum (Gibco); KM12-Luc, RPE-1 and TIG-3 were grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (Gibco).
3
Culturing Fibroblasts and Melanoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal human dermal fibroblast cells (NHDF, ATCC, Manassas, VA, USA) and melanoma cell lines (A2058, A375, WM115, MV3, ATCC) were cultured in Dulbecco's modified Eagle’s medium (DMEM, Gibco, Waltham, MA, USA) at a constant temperature (37 °C) with 5% CO2. The medium was contained 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Solarbio, Beijing, China).
4
Culturing Primary Dermal Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Normal Adult Human Primary Dermal Fibroblasts (NHDF) cells were purchased from ATCC (PCS-201-012, Manassas, VA, USA). NHDF cells were maintained in cultures in Dulbecco’s Modified Eagle’s Media (1:1) containing 10% fetal bovine serum and 1% antibiotic. NHDF cells were grown at 37°C in humidified 5% CO2.
5
Cell Culture Conditions for Common Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
293T, HeLa, U2OS and NHDF were obtained from ATCC. Mouse Embryonic Fibroblasts (MEFs) were established from E12.5 embryos. Cells were cultured at 37 °C in a 5% CO2 humidified incubator in Dulbecco's modified Eagle's medium (high glucose) supplemented with 15% fetal bovine serum (HANA-NESCO), 2 mM L -glutamine, 1% sodium pyruvate, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin. All the cells have been tested for mycoplasma contamination and turned out to be negative.
6
Culturing Adult Human Dermal Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Commercial human dermal fibroblasts (NHDF adult, Promocell, Heidelberg, Germany) were used for all cell experiments. NHDF fibroblasts were cultured in medium containing 72 % Dulbecco’s modified Eagle medium (ATCC, Manassas, USA), 18 % Medium M199 (Sigma-Aldrich, Steinheim, Germany), 9 % fetal calf serum (PAA Laboratories, Pasching, Austria) and 1 % Penicillin–Streptomycin (Invitrogen, Karlsruhe, Germany). Cells were incubated at 37 °C in humidified air with 5 % CO2 atmosphere. Culture medium was changed twice a week. Cells were subcultured by detachment with Accutase (PAA Laboratories, Pasching, Austria) at approximately 90 % confluence. Fibroblasts from the 2nd to 6th passage were used for all experiments.
7
Screening Membrane Transporter Inhibitors for HCMV
8
Culturing SKOV3, NHDF, and OVCAR3 Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SKOV3 and NHDF cell lines were purchased from ATCC, and SKOV3 was cultured in 10% FBS and 1% PS containing McCoy's 5a Medium. NHDF cells were cultured in 10% FBS and 1% PS containing DMEM Medium. The OVCAR3 cell line was gifted from the laboratory of Research Program in Systems Oncology at the University of Helsinki and cultured in 10% FBS, 1% PS, and 0.01 mg/mL bovine insulin containing RPMI-1640 Medium. All cells were maintained in the incubator of 37 °C and 5% CO2 atmosphere.
9
Cell Culture and Differentiation Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The current study was carried out using SH-SY5Y, PC12 and NHDF cell lines purchased from the ATCC (Manassas, VA, USA). The SH-SY5Y cells were differentiated with retinoic acid, and PC12 cells with NGF towards neuron-like cells. Cells were cultured in a humidified environment at 37 °C with 5% CO2 and passaged twice a week by transferring to the tube and centrifuging at 1000× g for 5 min. Then, the supernatants were removed, and cells were resuspended in fresh media. In the case of the PC12 cell line, cell clumps were broken by twofold squeezing through a 0.7 mm needle. In biological studies, PC12 cells were used at passages 11–16 and SH-SY5Y cell line was used at passages 9–14.
10
Anticancer Evaluation of Dipyridothiazine-Triazole Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All dipyridothiazines with 1,2,3-triazole substituents (1 –4)b –f were evaluated for their anticancer activity using four cultured cell lines: SNB-19 (human glioblastoma, DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), Caco-2 (human colon adenocarcinoma, DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), A549 (human lung carcinoma, ATCC, Manassas, VA, USA) and MDA-MB231 (human breast adenocarcinoma, ATCC, Manassas, VA, USA), and NHDF (human dermal fibroblast cell line, ATCC, Manassas, VA, USA. The cultured cells were kept at 37 °C and 5% CO2. The cells were seeded (1 × 104 cells/well/100 µL DMEM supplemented with 10% FCS and streptomycin and penicillin) using 96-well plates (Corning). The cells were counted in a hemocytometer (Burker’s chamber) using a phase contrast Olympus IX50 microscope equipped with Sony SSC-DC58 AP camera and Olympus DP10 digital camera.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!