7900ht light cycler

Manufactured by Thermo Fisher Scientific

Sourced in United States

The 7900HT Light Cycler is a real-time PCR instrument designed for accurate and reliable gene expression analysis. It features a high-performance optical system and advanced thermal control technology to deliver precise and reproducible results across a wide range of applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

7900HT cycler

4 protocols using 7900ht light cycler

Quantitative PCR and ChIP-qPCR Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primers used are listed in Table S3. Real-time PCR was performed in the presence of
SYBR Green using an Applied Biosystems 7900HT light cycler. For ChIP-qPCR, fold
enrichments were calculated using the ΔCT method and average values of
three independent experiments were expressed over the control set to 1. For
quantitative reverse transcription PCR (RT-qPCR), relative RNA levels were
calculated using the ΔCT method and normalized to act1levels.

Jain R., Iglesias N, & Moazed D. (2016). Distinct Functions of Argonaute Slicer in siRNA Maturation and Heterochromatin Formation. Molecular cell, 63(2), 191-205.

+ Open protocol

+ Expand

RT-PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were plated for 24 hr and treated as above. At designated times, cells were digested in Trizol reagent (Invitrogen, USA) and RNA was isolated. RNA purity and quantification were assessed using UV spectroscopy at the University of Iowa core facility. cDNA was synthesized using High Fidelity cDNA Synthesis Kits (Applied Biosystems, USA) following the manufacturer’s instructions. RT-PCR was performed using POWERUP SYBR green PCR master mix (ThermoFisher Scientific, USA) in a 7900HT light cycler (Applied Biosystems, USA). Relative mRNA expression for each treatment condition was calculated relative to β-actin. Oligonucleotide DNA primers were synthesized (Integrated DNA Technologies, USA) from published primer sequences (supplemental table 1)^{20 (link),25 (link),26 (link)}.

Seyedin S.N., Hasibuzzaman M., Pham V., Petronek M.S., Callaghan C., Kalen A.L., Mapuskar K.A., Mott S.L., Spitz D.R., Allen B.G, & Caster J.M. (2020). Combination therapy with radiation and PARP inhibition enhances responsiveness to anti-PD-1 therapy in colorectal tumor models.. International journal of radiation oncology, biology, physics, 108(1), 81-92.

+ Open protocol

+ Expand

Quantitative Analysis of Cytokine Expression in Mouse Liver

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was isolated from portions of mice liver by solubilisation in Trizol (Invitrogen Life Technologies, Carlsbad, CA, USA) and quantified using the Nanodrop 2000c spectrophotometer (Thermo Scientific, Waltham, MA, USA). For cDNA synthesis, 1 μg total RNA was transcribed using the High Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions. Quantitative Real Time PCR (qRT-PCR) analysis of IL-4, IL-5, IL-13, IL-10 and IFN-γ was performed using TaqMan specific probes (Applied Biosystems, Grand Island, NY, USA) (Table 1). Each sample was run in triplicate at 95 °C for 30 s followed by 40 cycles of 95 °C for 10 s and 60 °C for 30 s, using a Light Cycler 7900HT (Applied Biosystems). Data analysis was performed using the comparative threshold cycle (CT) method. Quantitative gene expression was calculated with the comparative CT method and normalized against the CT of the Glucuronidase (GUS) messenger reference gene, as previously used by Gong et al. [27 (link)].

Trinchese G., Paparo L., Aitoro R., Fierro C., Varchetta M., Nocerino R., Mollica M.P, & Berni Canani R. (2018). Hepatic Mitochondrial Dysfunction and Immune Response in a Murine Model of Peanut Allergy. Nutrients, 10(6), 744.

+ Open protocol

+ Expand

Quantification of Mucin and Tight Junctions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted with TRIzol reagent (Gibco BRL, Paisley, UK) and reverse transcribed in cDNA with a High-Capacity RNA-to-cDNA™ Kit (Life Technologies, Waltham, MA, USA) according to the manufacturer’s instructions. Complementary DNA (cDNA) was stored at −80°C until use. Quantitative real-time PCR (qRT-PCR) analysis was performed using Taqman Gene Expression Master Mix (Applied Biosystems, Vilnius, Lithuania) to evaluate the gene expression of mucin5AC (Muc5AC; Hs01365616_m1) and tight junction proteins occluding (Hs05465837_g1) and ZO-1 (Hs01551871_m1), CD137 (Hs00155512_m1), NFAT5 (Hs00232437_m1), AP1 (Hs99999141_s1), and Nf-kB1 (Hs00765730_m1). The TaqMan probes for these genes were inventoried and tested by Applied Biosystems manufacturing facility (QC). The amplification protocol was 40 cycles of 15 s of denaturation at 95°C, 60 s of annealing at 60°C, and 60 s of elongation at 60°C in a Light Cycler 7900HT (Applied Biosystems, Grand Island, NY, USA). Data were analyzed using the comparative threshold cycle method. We used the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene to normalize the level of mRNA expression.

Paparo L., Picariello G., Bruno C., Pisapia L., Canale V., Sarracino A., Nocerino R., Carucci L., Cosenza L., Cozzolino T, & Berni Canani R. (2021). Tolerogenic Effect Elicited by Protein Fraction Derived From Different Formulas for Dietary Treatment of Cow’s Milk Allergy in Human Cells. Frontiers in Immunology, 11, 604075.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!