Anti bcl 2 and anti bax antibodies

Oridonin was obtained from the Shaanxi Huike Botanical Development Co., Ltd., Shaanxi, China. Poly(lactic-co-glycolic)acid (PLGA, lactide/glycolide, 50:50, mol/mol, MW 10 kDa) was produced by Jinan Daigang Biomaterial Co., Ltd., Shandong, China. Gemcitabine, used as a positive control drug, was purchased from Hansoh Pharmaceutical Co., Ltd., Jiangsu, China. Polyvinyl alcohol (PVA, 87%–89% alcoholysis, MW 75000 Da) was purchased from the Aladdin Industrial Corporation, Shanghai, China, and ammonium bicarbonate was purchased from the Sinopharm Chemical Reagent Co., Ltd., Beijing, China. Cy7 was purchased from Fanbo Biochemicals Co., Ltd., Beijing, China. 3-Methyl cholanthrene (MCA, Sigma, USA), diethyl nitrosamine (DEN, Tokyo Chemical Industry, Japan), and iodized oil (Guerbet, French) were used. Anti-BCL-2 and anti-BAX antibodies were from the Cell Signaling Technology Inc. (Danvers, USA). All other chemicals and solvents were of analytical grade or high performance liquid chromatographic (HPLC) grade.

Doxorubicin hydrochloride (DOX·HCl) and CUR were purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Hyaluronic acid was purchased from Bloomage Freda Biopharm Co., Ltd (Jinan, Shandong, China). 4-Dimethylaminopyridine (DMAP), N,N-dimethylformamide (DMF) was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). N-Hydroxysuccinimide was purchased from Alfa Aesar (Ward Hill, MA, USA). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was purchased from Aladdin Reagent Database Inc. (Shanghai, China). All reagents were of HPLC or analytical grade and were used without further purification. Anti-P-gp antibody and an MDR assay kit (fluorometric) were purchased from Abcam (Cambridge, UK). Anti-Bcl-2 and anti-Bax antibodies were purchased from Cell Signaling Technology (Boston, MA, USA).