Anti cd16 32 fc block

Manufactured by BioXCell

Sourced in United States

The Anti-CD16/32 Fc-block is a laboratory reagent used to block non-specific binding of antibodies to Fc receptors during immunological experiments. It functions by binding to and occupying Fc receptors, preventing unwanted interactions with the Fc portion of antibodies.

Automatically generated - may contain errors

Lab products found in correlation

Zombie fixable viability kit, by BioLegend (2 mentions) Facsdiva software, by BD (2 mentions) Cd8 v500, by BD (1 mentions) Cd38 pe cy7, by BioLegend (1 mentions) Cd4 af700, by BD (1 mentions) Ccr7 pe cf594, by BD (1 mentions) Cd103 fitc, by BD (1 mentions) Cd11c bv711, by BioLegend (1 mentions) Cd14 bv650, by BioLegend (1 mentions) Cd45ra bv605, by BioLegend (1 mentions) Ccr5 pe, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Sh800, by Sony (1 mentions)

2 protocols using anti cd16 32 fc block

Comprehensive Immunophenotyping by Flow Cytometry

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All cells were stained for viability using the Zombie Fixable Viability Kit (Biolegend), incubated with anti-CD16/32 Fc-block (BioXcell), and stained with the indicated antibodies: CD19 APC, CD3 APC-H7 or BV450, CD4 AF700, CD8 V500, CCR7 PE-CF594, CD69 BV785, CCR5 PE, CD103 FITC, (BD Biosciences) CD38 PE-Cy7, CD11c BV711, CD14 BV650, CD45RA BV605 (Biolegend). Stained samples were run on an LSRII flow cytometer, data acquired using FACS DIVA software (BD Biosciences) and analyzed using FlowJo software (TreeStar, Inc., Ashland, Oregon).

Swaims-Kohlmeier A., Haaland R.E., Haddad L.B., Sheth A.N., Evans-Strickfaden T., Lupo L.D., Cordes S., Aguirre A.J., Lupoli K.A., Chen C.Y., Ofotukun I., Hart C.E, & Kohlmeier J.E. (2016). Progesterone levels associate with a novel population of CCR5+ CD38+ CD4 T cells resident in the genital mucosa with lymphoid trafficking potential. Journal of immunology (Baltimore, Md. : 1950), 197(1), 368-376.

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Flow Cytometric Sorting of CD45RA− CD4 T Cells

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Cell viability was determined using the Zombie Fixable Viability Kit (Biolegend^®, San Diego, California, USA) then incubated with anti-CD16/32 Fc block (BioXcell, Lebanon, New Hampshire, USA). Cells were measured using an LSRII flow cytometer or mechanically sorted using a Sony SH800. Flow data was acquired using FACS DIVA software (BD Biosciences, Franklin Lakes, New Jersey, USA) and analyzed using FlowJo software (TreeStar, Inc., Ashland, Oregon, USA). For cell sorting, viable lymphocyte-gated singlet cells were discriminated for IV-CD45 (PE) negative and CD45+ (BV450) followed by CD3+ and CD4+. Next, CD45RA− CD4 T cells were sorted by CCR7 expression. The following fluorochrome-conjugated antibodies were used in this study:

Williams M.E., Hardnett F.P., Sheth A.N., Wein A.N., Li Z.R., Radzio-Basu J., Dinh C., Haddad L.B., Collins E.M., Ofotokun I., Antia R., Scharer C.D., Garcia-Lerma J.G., Kohlmeier J.E, & Swaims-Kohlmeier A. (2023). The menstrual cycle regulates migratory CD4 T-cell surveillance in the female reproductive tract via CCR5 signaling. Mucosal immunology, 17(1), 41-53.

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