Goat anti mouse af546

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Goat anti-mouse AF546 is a secondary antibody conjugated with the Alexa Fluor 546 fluorophore. It is designed to detect and bind to mouse primary antibodies, allowing for fluorescent labeling and visualization of target molecules.

Automatically generated - may contain errors

Lab products found in correlation

Zen 2012, by Zeiss (1 mentions) Axiocam icc1 camera, by Zeiss (1 mentions) Pgem t easy vector, by Promega (1 mentions) Mouse anti cdh1, by BD (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions) Stemi 2000 c, by Zeiss (1 mentions) Treat 8 well μ slides, by Ibidi (1 mentions) Tcs sp5, by Leica (1 mentions) Goat anti rabbit af488, by Thermo Fisher Scientific (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions) Rabbit anti vwf, by Agilent Technologies (1 mentions)

Spelling variants of this product

AF546 (15 citations) Anti-mouse AF546 (6 citations)

2 protocols using goat anti mouse af546

In Situ Hybridization and Immunostaining Protocol

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The protocol of ISH was previously described (16 (link)). Specific regions of lefty1 and foxj1a were cloned into pGEM T easy vector (Promega). ISH probe vectors for cmlc1, dand5, spaw, and pan-akap12 were previously described (12 (link), 16 (link)) and primer sequences of lefty1 and foxj1a for ISH probe vectors are summarized in Supplementary Table S1. The protocol of whole-mount immunostaining for Tg(sox17:egfp) embryos was described previously (16 (link)). Mouse anti-Cdh1 (1:200, BD Biosciences) and goat anti-mouse AF546 (1:1000, Invitrogen) were used for immunofluorescence. Stained embryos were mounted in glycerol and images were obtained by an AxioCam ICC-1 camera (Zeiss) on a Stemi 2000C (Zeiss) for immunohistochemistry and an LSM700 confocal microscope (Zeiss) for immunofluorescence, respectively, and processed using ZEN 2012 software (Zeiss).

Kim J.G., Kim H.H, & Bae S.J. (2019). Akap12beta supports asymmetric heart development via modulating the Kupffer’s vesicle formation in zebrafish. BMB Reports, 52(8), 526-531.

+ Open protocol

+ Expand

Immunofluorescence Analysis of VWF in HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence studies were performed with transfected HEK293 as previously described [26 (link)]. HEK293 cells were seeded in Ibidi treat 8-well μ-slides (Ibidi, Martinsried, Germany) 24h prior transfection with Lipofectamine LTX (Invitrogen) and VWF-plasmid-constructs in vector pcDNA3 [5 (link)]. Antibodies used were: rabbit anti-VWF (DAKO, Hamburg, Germany, 1:1,000), mouse anti-Protein Disulfide Isomerase (PDI) (Abcam, Cambridge, UK, 1:500), goat anti-rabbit AF488 (Invitrogen, 1:5,000), goat anti-mouse AF 546 (Invitrogen, 1:5,000). Images were captured at RT with a confocal microscope (TCS SP5, Leica, Wetzlar, Germany). For settings, please refer to legend of Fig. 4.

Obser T., Ledford-Kraemer M., Oyen F., Brehm M.A., Denis C.V., Marschalek R., Montgomery R.R., Sadler J.E., Schneppenheim S., Budde U, & Schneppenheim R. (2016). Identification and characterization of the elusive mutation causing the historical von Willebrand Disease type IIC Miami. Journal of thrombosis and haemostasis : JTH, 14(9), 1725-1735.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!