Log In Sign up
The largest database of trusted experimental protocols

Led driver

Manufactured by Thorlabs
Visit Supplier
Sourced in United States, Jersey

The LED driver is a device that supplies the necessary voltage and current to power light-emitting diodes (LEDs). It regulates the input power to ensure stable and efficient LED operation.

Automatically generated - may contain errors

Lab products found in correlation

Optic fiber, by Thorlabs (1 mentions) Uplanfl, by Olympus (1 mentions) Bergamo 2 scope, by Thorlabs (1 mentions) Zyla 5.5 scmos camera, by Oxford Instruments (1 mentions) Blue led source, by Thorlabs (1 mentions) Digidata 1440 dac, by Molecular Devices (1 mentions) Pclamp 10, by Molecular Devices (1 mentions) Synapse software, by Tucker-Davis Technologies (1 mentions) Med pc 5 software, by Med Associates (1 mentions) Blue led, by Doric (1 mentions) Matlab code, by MathWorks (1 mentions) Fluorescence minicube, by Doric (1 mentions)

Spelling variants of this product

T-Cube LED Driver (16 citations) LEDD1B T-Cube LED Driver (4 citations) DC2200 LED Driver (3 citations) DC2200 – High‐Power 1‐ Channel LED Driver (3 citations) DC4100 4-Channel LED Driver (3 citations) DC2100 LED driver (3 citations) Single channel LED driver (2 citations) High Power LED driver (2 citations) T-Cube LED Driver 1200 mA (2 citations)

8 protocols using led driver

1

Optogenetic Stimulation of Cerebellar Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
LED photostimulation (λ = 470 nm) driven by a Thorlabs LED driver (225 μW) was given through an optic fiber (400 μm in diameter, Thorlabs, Newton, NJ, USA). The optic fiber rested on the dura mater above the midline between crus 1, crus 2, approximately 1 mm lateral from the vermis, via the craniotomy. During experiments with optogenetic stimulation, trials without stimulation, with 100 ms and with 200 ms optogenetic stimulation were randomly intermingled.
Romano V., Reddington A.L., Cazzanelli S., Mazza R., Ma Y., Strydis C., Negrello M., Bosman L.W, & De Zeeuw C.I. (2020). Functional Convergence of Autonomic and Sensorimotor Processing in the Lateral Cerebellum. Cell Reports, 32(1), 107867.
+ Open protocol
+ Expand
2

Wide-Field Calcium Imaging of Mouse Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Wide-field 1-photon and two-photon calcium imaging was performed using the Bergamo II Scope (Thorlabs) with blue LED illumination using an LED driver (Thorlabs). GCaMP6s fluorescence signal from the cortical surface was acquired at about 15 Hz (640 × 540 pixels, field of view (FOV) ranges from 3×3 to 5×5 mm2) using a Zyla 5.5 sCMOS camera (Andor) controlled by μManager2. Average excitation power at the exit of the air objective (2x or 4x, UPlanFl, Olympus) ranged from 0.2 to 0.8 mW. Individual sessions were initially registered prior to recording using a blood vessel template recorded on the first chronic session. More accurate registration was performed offline using custom scripts based on pixel correlation. Single-condition response maps were expressed as ΔFF=Fstim−FoFo where Fstim is the trial and time-averaged response across stimulus frames, and Fo is the trial and time-averaged response during the 1 second prior to stimulus onset.
Sedigh-Sarvestani M., Lee K.S., Jaepel J., Satterfield R., Shultz N, & Fitzpatrick D. (2021). Sinusoidal transformation of the visual field is the basis for periodic maps in area V2. Neuron, 109(24), 4068-4079.e6.
+ Open protocol
+ Expand
3

Optogenetic Activation of TRPV1+ Nociceptors

Check if the same lab product or an alternative is used in the 5 most similar protocols
To activate TRPV1+ nociceptors by blue light (473 nm; power 4.7 mW; frequency 3 Hz; 20% duty cycle, pulse width 67ms), a LED driver (Thor Labs, Newton, New Jersey) was connected to a blue LED source (Thor Labs). Parameters previously shown to demonstrate hypersensitivity in the paw for up to 24 h were used (Daou et al. 2013 (link)). The power from the LED was measured using a power meter with the LED positioned at the same 1 cm distance from the sensor at the start and end of the experiment. The power measured from these settings at both points was 4.7mW and the pulse width was 67ms. The LED was positioned over the right hind paw at 1 cm distance from the skin (Fig. 1A). TRPV1-Cre/ChR2 mice or C57Bl6 mice were stimulated with blue light on the hind paw for 15 min. Immediately following optogenetic stimulation, mice were injected with 3 mg/kg of KLH subcutaneously in 20 µL into the same paw. Blood was collected weekly, and serum antibody titers tested.
Tynan A., Tsaava T., Gunasekaran M., Bravo Iñiguez C.E., Brines M., Chavan S.S, & Tracey K.J. (2024). TRPV1 nociceptors are required to optimize antigen-specific primary antibody responses to novel antigens. Bioelectronic Medicine, 10, 14.
+ Open protocol
+ Expand
4

Optogenetic Stimulation of EPSPs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Electrophysiology recording with optogenetics stimulation were performed with the same set up detailed above with a ×40/1.0 NA water immersion objective. Dissection and electrophysiological recordings were performed in the same modified HL-3 saline described above. To stimulate EPSP events, 0.5 ms light pulses of 470 nm were delivered from an LED driver (Thorlabs) at 0.5 Hz, triggered by a TTL pulse driven from a Digidata 1440 DAC programmed using pClamp 10.5 software (Molecular Devices). Light intensity was controlled by the LED driver to avoid multiple EPSP events. The power range were calibrated between 5 and 20 mW/cm2 under the objective.
Han Y., Chien C., Goel P., He K., Pinales C., Buser C, & Dickman D. (2022). Botulinum neurotoxin accurately separates tonic vs. phasic transmission and reveals heterosynaptic plasticity rules in Drosophila. eLife, 11, e77924.
+ Open protocol
+ Expand
5

Threshold Light Sensitivity in Somatostatin Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
To measure the ‘threshold’ light intensity at which somatostatin mice began responding to stimulation, mice were placed in individual tubes with cannulae attached and allowed to first habituate for 30 minutes. Following habituation, each mouse was sequentially screened for approximate current driving a Thorlabs LED driver at which they flinched. Once an approximate current was established, testing began 30–40 mA below that threshold. A stopwatch was used to measure the latency until mice attended the stimulus (which was a lick response in all cases). A cutoff value of 20 s was chosen (i.e. stimulus did not continue more than 20 seconds). At 5 minute intervals, LED intensity was increased by 10 mA. Once the time to attendance dropped below 1 second, tests were halted. The current value at which the mouse response time first dropped below 20 s was chosen as the ‘threshold’ value, and was used in future experiments with that specific mouse. These current values were then normalized to ‘threshold’, and plotted against threshold plus or minus a delta (10 mA steps).
Christensen A.J., Iyer S.M., François A., Vyas S., Ramakrishnan C., Vesuna S., Deisseroth K., Scherrer G, & Delp S.L. (2016). In vivo interrogation of spinal mechanosensory circuits. Cell reports, 17(6), 1699-1710.
+ Open protocol
+ Expand
6

Fiber Photometry System for Neural Activity Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols
The fiber photometry system used two light-emitting diodes (490 nm and 405 nm; Thorlabs) controlled by an LED driver (Thorlabs). The 490 nm light source was filtered with a 470 nm (the excitation peak of dLight1.1) bandpass filter and the 405nm light source was used as an isosbestic control.48 (link) Light was passed through an optical fiber (400 μm, .48 NA; Doric) that was coupled to a chronically implanted fiber optic cannula in each mouse. LEDs were controlled via a real-time signal processor (RZ5P; Tucker-Davis Technologies) and emission signals from each LED were determined by multiplexing. Synapse software (Tucker-Davis Technologies) was used to control the timing and intensity of the LEDs and to record the emitted fluorescent signals upon detection by a photoreceiver (Newport Visible Femtowatt Photoreceiver Module; Doric). LED power (125 μW) was measured daily and maintained across trials and experiments. For each event of interest (e.g., cue presentation, footshock), transistor-transistor logic (TTL) signals were used to timestamp onset times from Med-PC V software (Med Associates Inc.) and were detected via the RZ5P in the Synapse software (see below). A built-in low-pass filter on the Synapse software was set to 10 Hz to eliminate noise in the fiber photometry raw data.
Philipp M., Kohnen R, & Hiller K.O. (1999). Hypericum extract versus imipramine or placebo in patients with moderate depression: randomised multicentre study of treatment for eight weeks. BMJ : British Medical Journal, 319(7224), 1534-1539.
+ Open protocol
+ Expand
7

Optogenetic Silencing of V1 in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
An optical fiber (1 mm) coupled to a blue LED (470 nm; Doric Lenses) was placed over V1 above the intact skull covered with a thin layer of Krazy glue. The fiber was placed at approximately the retinotopic location corresponding to the stimulus during the initial 350 ms in the task: ~2.3 mm from midline and ~1.3 mm from lambdoid suture. To find these coordinates, we recorded multiunit activity in V1 with the monitor in the same position as during optogenetic silencing (monitor was moved <15 degrees to center the spatial receptive field of the multiunit activity). We used these same approximate coordinates for all of our recordings.
For each animal, the total power was increased until the performance was at chance level when the LED illumination started before the stimulus appeared (3.3–20 mW across animals; p>0.05; Wilcoxon ranksum test on stimulus centering times in the reward zone). To turn the LED on at specific delays after stimulus onset, the photodiode signal detecting the onset of the stimulus was sent to an amplifier (Newark; TWLUX - TW-MF2CAB) and then to an external microprocessor (Mega 1280; Arduino). The microprocessor waited for the amplified photodiode signal to cross a threshold before sending out a digital trigger to the LED driver (Thorlabs). The jitter (s.d.) of the LED onset was 4 ms.
Resulaj A., Ruediger S., Olsen S.R, & Scanziani M. (2018). First spikes in visual cortex enable perceptual discrimination. eLife, 7, e34044.
+ Open protocol
+ Expand
8

Fiber Photometry for Calcium Imaging

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Calcium imaging was performed using a custom-built fiber photometry rig based on the design of the Deisseroth (Lerner et al. 2015 (link)) and Woodward labs (Braunscheidel et al. 2019 (link)). An LED driver (Thorlabs, Newton, New Jersey) provided both 405 nm and 490 nm illumination which were combined in a fluorescence mini-cube (Doric Lenses, Québec, QC, Canada). A custom made 400 μm diameter patch cord terminating in a ceramic sleeve was used to connect the mini-cube to the animal’s fiber optic implant. Synapse software was used to control a digital processor that received input from a photodetector that collected emission signals at both 405 and 490 nm (Tucker-Davis Technologies, Alachua, FL). Integrated TTL signals were used to time-lock neuronal recordings to the start of each fear conditioning session and signaled the start of each tone throughout the session. Custom-written MatLab codes were used to analyze these data (The MathWorks Inc.). Signals from the 405 and 490 nm channels were subtracted from each other to calculate ΔF/F, and data for each testing day was then z-normalized to a combined baseline of all signaling across the whole session for that day.
Smiley C.E., Saleh H.K., Nimchuk K.E., Garcia-Keller C, & Gass J.T. (2021). Adolescent exposure to delta-9-tetrahydrocannabinol and ethanol heightens sensitivity to fear stimuli. Behavioural brain research, 415, 113517.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!