Quattro ultima

Manufactured by Waters Corporation

Sourced in United Kingdom

The Quattro Ultima is a high-performance mass spectrometer designed for advanced analytical applications. It features a triple quadrupole configuration, enabling precise quantitative and qualitative analysis of a wide range of compounds. The Quattro Ultima provides reliable and consistent performance, making it a versatile tool for various industries and research disciplines.

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Lab products found in correlation

Pgb2 d4, by Cayman Chemical (4 mentions) 12 hete d8, by Cayman Chemical (4 mentions) Kinetex c18 column, by Phenomenex (1 mentions) Luna c18 column, by Phenomenex (1 mentions) Waters alliance 2695, by Waters Corporation (1 mentions) 2690 separation module, by Waters Corporation (1 mentions) Masslynx 4, by Waters Corporation (1 mentions) C18 e cartridges, by Phenomenex (1 mentions) Luna 5 μm, by Phenomenex (1 mentions) Qtof2, by Waters Corporation (1 mentions) Pgb2 d4 40 ng, by Cayman Chemical (1 mentions) Alliance 2695, by Waters Corporation (1 mentions) Qtof 2 mass spectrometer, by Waters Corporation (1 mentions)

14 protocols using quattro ultima

LC-MS/MS Quantification of Paclitaxel

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Liquid chromatography conditions were as follows: C₁₈ (HILIC) column (Nucleodur, EC 125/2, 100-5-C18, Macherey-Nagel,
Hoerdt, France). Mobile phase: acetonitrile/water (50/50, v/v) with
formic acid 0.1%; run time: 8 min; flow rate: 0.3 mL min^–1. ESI-MS/MS analyses were performed on a triple quadrupole mass spectrometer
detector (TQD) with an electrospray ionization (ESI) interface (Quattro
Ultima, Waters, Guyancourt, France). Electrospray and mass parameters
were optimized by direct infusion of pure analytes into the system.
ESI parameters: capillary voltage of 3.5 kV, cone voltage of 35 V,
source temperature of 120 °C, desolvation temperature of 350
°C, with a nitrogen flow of 506 L h^–1. Mass
parameters: transitions were monitored as follows: Ptx 854/286; Ptx-d₅ 859/291. Calibration: calibration curve was linear in the
range 5–1000 ng mL^–1 (y =
0.0047x + 0.0838; R² =
0.9936 in PBS and y = 0.0052x –
0.0131; R² = 0.9949 in mouse plasma).

Bordat A., Boissenot T., Ibrahim N., Ferrere M., Levêque M., Potiron L., Denis S., Garcia-Argote S., Carvalho O., Abadie J., Cailleau C., Pieters G., Tsapis N, & Nicolas J. (2022). A Polymer Prodrug Strategy to Switch from Intravenous to Subcutaneous Cancer Therapy for Irritant/Vesicant Drugs. Journal of the American Chemical Society, 144(41), 18844-18860.

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Quantification of Lipid Mediators in Plasma

Cited 2 times

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Lipid mediators in human plasma were analysed by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) based on protocols published previously [12] (link), [13] (link). Briefly, samples were collected and stored immediately at −80°C. Plasma samples (500 µL) were defrosted on ice and adjusted to 15% (v/v) methanol: water (final volume 4 mL). Internal standards, PGB2-d4 (40 ng) and 12-HETE-d8 (40 ng) (Cayman Chemical Company, Ann Arbor, USA) were added and the pH of resulting solutions adjusted to 3.0 (1 M HCL). Acidified samples were immediately applied to preconditioned solid-phase cartridges (C18-E, Phenomenex, Macclesfield, UK) and lipid mediators eluted with methyl formate. LC/ESI-MS/MS analysis was performed on a HPLC pump (Waters Alliance 2695) coupled to an electrospray ionisation triple quadrupole mass spectrometer (Quattro Ultima, Waters, UK). Chromatographic separation was performed on a C18 Luna column (5 µm, 150×2.0 mm, 21 Phenomenex) for eicosanoids and a C18 Kinetex column (2.6 µm, 100×2.1 mm, Phenomenex) for hydroxy-fatty acids. Analytes were monitored on multiple reaction monitoring mode as reported [12] (link), [13] (link) with the following additions: 15-hydroxyeicosatrienoic acid (HETrE) m/z 321>221, 10-hydroxydocosahexaenoicacid (HDHA) m/z 343>153, 14-HDHA m/z 343>161, 13-HDHA m/z 343>193 and 17- HDHA m/z 343>201.

Jenner W., Motwani M., Veighey K., Newson J., Audzevich T., Nicolaou A., Murphy S., MacAllister R, & Gilroy D.W. (2014). Characterisation of Leukocytes in a Human Skin Blister Model of Acute Inflammation and Resolution. PLoS ONE, 9(3), e89375.

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Quantitative Plasma VCM Determination

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The VCM assay was performed by a previously reported liquid chromatography/tandem mass spectrometry (LC/MS/MS) method (Shibata et al. 2003 (link)) with some modifications; briefly, a 100 µL plasma sample was added to 60 µL of 30 % trifluoroacetic acid to precipitate protein. After vortexing and centrifugation at 12,000 rpm for 15 min, the supernatant was diluted in 340 µL of distilled water and was passed through a filter, and 10 µL of the filtrate was injected into a Quattro Ultima LC/MS/MS system with a 2690 Separation Module (Waters Co, MA, UK). VCM separation was performed with a QUICKSORB ODS column (i.d. 2.1 mm × 100 mm, 3 µm, Chemco Scientific Co., Ltd., Osaka, Japan), and the elution was carried out isocratically at a flow rate of 0.2 mL/min with the degassed mobile phase, acetonitrile: 0.1 % acetic acetate (2:8). Mass spectrometry was conducted with electrospray ionization in positive mode (ESI+) under the following conditions: source temperature, 130 °C; cone voltage, 35 V; capillary voltage, 4.0 kV. VCM intensity was monitored by multiple reaction monitoring (MRM) with 18 eV of collision energy for the VCM transition (725–144 m/z). VCM concentration was quantified by calculating peak area against calibrated samples. The lower limit of quantitation for VCM was <0.005 µg/mL.

Fukushima K., Okada A., Hayashi Y., Ichikawa H., Nishimura A., Shibata N, & Sugioka N. (2015). Enhanced oral bioavailability of vancomycin in rats treated with long-term parenteral nutrition. SpringerPlus, 4, 442.

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Quantitative Analysis of PHNQ using IS

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For the quantitative part of the study, the internal standard (IS) method was selected and 2-hydroxy-1,4-naphthoquinone was privileged as the IS based on the similarity between its chemical structure and the PHNQ. The IS stock solution was prepared in 80% methanol at a concentration of 10 µg ml⁻¹ and directly added to the dried sample. The mass spectra were obtained on a Waters Quattro Ultima using the ESI source in the negative mode by scanning between m/z 50 and 1500. The ESI conditions were as follows: capillary voltage of 3.1 kV, cone voltage of 40 V, source temperature at 120°C and desolvation temperature at 300°C. Dry nitrogen was used as the ESI gas with a flow rate of 50 l h⁻¹ for the gas cone and 500 l h⁻¹ for the desolvation gas.

Brasseur L., Demeyer M., Decroo C., Caulier G., Flammang P., Gerbaux P, & Eeckhaut I. (2018). Identification and quantification of spinochromes in body compartments of Echinometra mathaei’s coloured types. Royal Society Open Science, 5(8), 171213.

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Quantification of Plasma α-Gal A and LysoGb3

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Plasma α-Gal A activity was determined using the substrate 4-methylumbelliferyl α-D-galactopyranoside (5 mmol/L) freshly prepared in 117 mmol/L N-acetyl-D-galactosamine/50 mmol/L citric-phosphate buffer, pH 4.6, before every assay. In brief, 50 μL of plasma was mixed with 300 μL of the substrate solution, incubated at 37°C for 2 hours, and 0.2 N glycine-NaOH was added to stop the reaction. Fluorescence intensity was measured with the excitation and emission wavelengths of 365 and 450 μm, respectively [27 ,28 (link)]. Plasma LysoGb3 was detected by tandem mass spectrometry that was performed in positive ion mode (ES+) on a triple quadruple mass spectrometer (Quattro Ultima, Waters, Milford, MA) with NeoLynx software version 4.1. A multiple reaction monitoring (MRM) mode was used for the measurement of lysoGb3. The analyzing methods were modified from the protocol provided by Shire [29 (link)].

Hsu T.R., Sung S.H., Chang F.P., Yang C.F., Liu H.C., Lin H.Y., Huang C.K., Gao H.J., Huang Y.H., Liao H.C., Lee P.C., Yang A.H., Chiang C.C., Lin C.Y., Yu W.C, & Niu D.M. (2014). Endomyocardial biopsies in patients with left ventricular hypertrophy and a common Chinese later-onset fabry mutation (IVS4 + 919G > A). Orphanet Journal of Rare Diseases, 9, 96.

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UPLC-MS/MS Analysis of Urolithin Metabolites

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Urine and plasma samples were thawed and extracted for UPLC-MS/MS analysis following previously reported protocol [43 ]. UPLC eluate was introduced to a triple quadrupole mass spectrometer (Quattro Ultima, Waters Corp., Beverley, MA) via an electrospray mode operated in positive ion mode without splitting flow. Standards of urolithin A, B, C, D, methyl A, and dimethyl A were used for external calibration [43 ]. Baseline urine analysis was determined from a spot urine collection and metabolite profiles were normalized to urinary creatinine levels. Final urinary analysis was based on a 24-hour urine collection and metabolite profiles were normalized to 24-hour urine volumes.

Roberts K.M., Grainger E.M., Thomas-Ahner J.M., Hinton A., Gu J., Riedl K., Vodovotz Y., Abaza R., Schwartz S.J, & Clinton S.K. (2020). Dose-dependent increases in ellagitannin metabolites as biomarkers of intake in humans consuming standardized black raspberry food products designed for clinical trials. Molecular nutrition & food research, 64(10), e1900800.

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Quantitative Lyso-Gb3 Analysis in Plasma

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The preparation and analysis of the samples was described in our previous publication [13 (link)]. Control samples were obtained from 31 healthy adults (16 males and 15 females). Serum lyso-Gb3 level <0.5 nM was defined normal. All the individuals in the control group had normal enzyme activity and no known GLA gene mutation.
Patient’s blood was used for lyso-Gb3 analysis. Plasma samples with 50 μL or calibration standards and internal standards were added into 96 wells plate. Lipid was extracted from plasma via chloroform, methanol and formic acid. Centrifuging was carried out several times during the extraction. Stepwise gradient elusion was done via a liquid chromatography–mass spectrometry system (Waters Alliance 2795XE HPLC, USA). Tandem mass spectrometry detection of lyso-Gb3 was performed on a triple quadruple mass spectrometer (Quattro Ultima, Waters, Milford, MA) with NeoLynx software version 4.1. Multiple reaction monitoring mode (MRM) was used for lyso-GB3 measurement.
In lyso-Gb3 analysis, we used lower (5 nM) and higher (100 nM) lyso-Gb3 spiked plasma as quality controls. Our laboratory’s coefficient of variation (CV) of inter-assay was 9.2% for the upper control and 11.0% for the lower control. As for intra-assay’s CV, it was 12.8% for the upper control and 9.9% for the lower control.

Liu H.C., Lin H.Y., Yang C.F., Liao H.C., Hsu T.R., Lo C.W., Chang F.P., Huang C.K., Lu Y.H., Lin S.P., Yu W.C, & Niu D.M. (2014). Globotriaosylsphingosine (lyso-Gb3) might not be a reliable marker for monitoring the long-term therapeutic outcomes of enzyme replacement therapy for late-onset Fabry patients with the Chinese hotspot mutation (IVS4+919G>A). Orphanet Journal of Rare Diseases, 9, 111.

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Quantifying Skin Eicosanoids by LC-MS/MS

Cited 1 time

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Eicosanoids in skin blister fluid were quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry as described previously (30 (link)–32 (link)). Briefly, skin fluid samples (typically 50–200 μL) were diluted with methanol-water (15% w/w) up to 3 mL. Internal standards (40 ng PGB₂-d4 and 80 ng 12-HETE-d8; Cayman Chemicals) were then added and resultant solutions acidified to pH 3.0, followed by solid-phase extraction (C18-E cartridges; Phenomenex) to reduce matrix effects and semi-purify the lipid mediators. Eicosanoids were analyzed on a C18 column (Luna 5 μm; Phenomenex) by using a Waters Alliance 2695 HPLC pump coupled to a triple-quadrupole mass spectrometer equipped with an electrospray ionization probe (Quattro Ultima; Waters). Instrument control and data acquisition were performed by using MassLynx 4.0 software (Waters). Multiple-reaction monitoring transitions used were as follows: PGE₂, m/z 351 > 271; PGE₁, m/z 353 > 317; PGE₃, m/z 349 > 269; PGJ₂, m/z 333 > 271; PGD₁, m/z 353 > 317; PGD₂, m/z 351 > 271; PGF_2α, m/z 353 > 193; 13,14-dihydro-15-keto PGE₂, m/z 351 > 333; 13,14-dihydro-15-keto-PGE₁, m/z 353 > 335; 12-HETE, m/z 319 > 179; 11-HETE, m/z 319 > 167; 15-HETE, m/z 319 > 175; 15-hydroxyeicosatrienoic acid, m/z 321 > 221; 9-hydroxyoctadecadienoic acid, m/z 295 > 171; and 13-hydroxyoctadecadienoic acid, m/z 295 > 195.

Farrar M.D., Nicolaou A., Clarke K.A., Mason S., Massey K.A., Dew T.P., Watson R.E., Williamson G, & Rhodes L.E. (2015). A randomized controlled trial of green tea catechins in protection against ultraviolet radiation–induced cutaneous inflammation. The American Journal of Clinical Nutrition, 102(3), 608-615.

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Comprehensive Geochemical Analysis of Environmental Samples

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Level-1 analyses included geochemical
parameters comprising determination of anions (including chloride,
sulfate, and nitrate) analyzed by ion chromatography, major cations
(including sodium and calcium) analyzed by direct aspiration using
an inductively coupled argon plasma mass spectrometry, and ammonium
analyzed by spectrophotometry (absorbance of phenol-hypochlorite at
640 nm^{16 (link)}). Alkalinity was determined by
titration method 2320.⁵⁰ Samples were analyzed
for a suite of trace metals at Environment and Climate Change Canada’s
National Laboratory for Environmental Testing (NLET) (Burlington,
ON) using inductively coupled plasma-sector field mass spectrometry
(SOP 2003). Level-1 analyses also included determination of total
AEO concentrations (referred to subsequently as NAs). Low resolution
ESI-MS analyses for total NAs were conducted with a Quattro Ultima
(Waters Corp., Milford, MA) triple quadrupole mass spectrometer equipped
with an ESI interface operating in negative-ion mode, as described
by Frank et al.^{16 (link)} Additionally, expected
maxima in an SFS profile associated with previously identified mono-
and diaromatic acids^{39 (link)} were analyzed as
described previously.^{51 (link)} In this investigation,
samples that exhibited the characteristic bitumen profile with three
maxima with a signal intensity of 100 at 272 nm were identified as
positive for this profile.

Hewitt L.M., Roy J.W., Rowland S.J., Bickerton G., DeSilva A., Headley J.V., Milestone C.B., Scarlett A.G., Brown S., Spencer C., West C.E., Peru K.M., Grapentine L., Ahad J.M., Pakdel H, & Frank R.A. (2020). Advances in Distinguishing Groundwater Influenced by Oil Sands Process-Affected Water (OSPW) from Natural Bitumen-Influenced Groundwaters. Environmental Science & Technology, 54(3), 1522-1532.

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Skin Eicosanoid Quantification by LC-MS/MS

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Analysis was performed as described previously 9 . Briefly, skin samples (5-20mg) were homogenised in ice-cold methanol (15%v/v) with internal standards added to each sample (40ng each of 12-HETE-d8 and PGB 2 -d4; Cayman Chemicals, Ann Arbor, MI, USA).
Homogenates were semi-purified by solid phase extraction (SPE; C18-E; Phenomenex, Macclesfield, UK) and eicosanoids analysed by LC/ESI-MS/MS using a triple quadrupole mass spectrometer with electrospray probe coupled to liquid chromatography (Quattro Ultima, Waters, Elstree, Hertfordshire, UK). Data are expressed as pg/mg protein.

Kendall A.C., Pilkington S.M., Sassano G., Rhodes L.E, & Nicolaou A. (2016). N-Acyl ethanolamide and eicosanoid involvement in irritant dermatitis. The British journal of dermatology, 175(1).

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