X-ray diffraction (XRD) studies were carried out with a Nissan Rigaku D/MAX RA X-ray polycrystalline diffractometer under the following conditions: the source was a copper target (Cu Kα, λ = 0.15418 nm), and the scan range was 10°–80°. The specific surface areas and pore structures were determined with the JW-BK132F Beijing Jingwei Gao Bo Automatic Static Volume Method for Specific Surface and Pore Size Analyses with N2 adsorption at 77 K. The specific surface area, pore volume and pore size distributions of the catalyst were obtained with the Brunauer–Emmett–Teller (BET) and BJH models, respectively. Scanning electron microscopy (SEM) with a Phillips XL-30-ESEM scanning electron microscope was used to observe the catalysts. Transmission electron microscopy (TEM) was performed with a JEM-2010 system from JEOL, Japan, with an accelerating voltage of 160 kV. Fourier transform infrared spectroscopy (FT-IR) was carried out with a Bruker Tensor 27 r. The spectral range was 4000–600 cm-1, and 32 scans were collected. The catalyst was mixed with KBr and pressed into tablets for the FT-IR analyses. X-ray photoelectron spectra (XPS) were measured with a Thermo ESCALAB 250 photoelectron spectrometer using a monochromatic Al Kα irradiation source (hv = 1,486.6 eV), a power of 150 W, a 500 μm beam spot, and an energy analyser with a fixed transmission energy of 30 eV.
Jem 2010 system
Manufactured by JEOL
Sourced in Japan
The JEM 2010 system is a transmission electron microscope (TEM) designed and manufactured by JEOL. It is a versatile and high-performance instrument capable of providing detailed images and structural analysis of a wide range of materials at the nanoscale level. The core function of the JEM 2010 system is to enable users to obtain high-resolution, high-contrast electron micrographs and perform advanced analytical techniques such as electron diffraction and energy-dispersive X-ray spectroscopy.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250 photoelectron spectrometer, by Thermo Fisher Scientific (2 mentions)
Xl 30 esem scanning electron microscope, by Philips (1 mentions)
Cary eclipse, by Agilent Technologies (1 mentions)
Optizen popuv vis spectrophotometer, by Mecasys (1 mentions)
90 plus particle size analyzer, by Brookhaven Instruments (1 mentions)
Particle size analyzer, by Brookhaven Instruments (1 mentions)
Zetasizer nano, by Malvern Panalytical (1 mentions)
Gel doc ez system, by Bio-Rad (1 mentions)
8 protocols using jem 2010 system
1
Comprehensive Characterization of Catalysts
2
Characterization of SiO2@InP QDs@SiO2 NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) images of SiO2@InP QDs@SiO2 NPs were obtained using a JEM-2010 system (JEOL, Tokyo, Japan). UV/Vis/NIR absorbance spectra of the SiO2@InP QDs@SiO2 NPs were obtained using a Optizen Pop UV/Vis spectrophotometer (Mecasys, Daejeon, Korea). Photoluminescence (PL) emission spectra of the SiO2@InP QDs@SiO2 NPs were obtained using a Cary Eclipse (Agilent Technologies, Santa Clara, CA, USA). The quantum yield (QY) of the SiO2@InP QDs@SiO2 NPs was measured using a QE-2000 (Otsuka Electronics, Osaka, Japan).
3
Characterization of PEI-Et/siRNA Polyplexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A PEI-Et solution was added into siRNA solution in polymer-to-siRNA N/P ratios of 5, 10, 15, 25, 30, 40, 50, 75, and 100 to prepare PEI-Et/siRNA polyplexes, which were then incubated for 30 min at room temperature. A 1.0% agarose gel with 0.5 μg/mL ethidium bromide was used to load the formed siRNA nanoparticles and then subjected to electrophoresis for 30 min at 90 V. The condensing efficiency was identified by the retardation of siRNA by visualization under an UV illuminator. A Brookhaven 90Plus particle size analyzer was used to measure the particle size, the zeta potential of the nanoparticles, and the distribution of the polyplex at various N/P ratio in water. Three individual experiments were conducted to calculate the values of the zeta potentials and the particle sizes. A transmission electron microscope (TEM, JEM 2010 system JEOL, Japan) was used to observe the images of the PEI-Et siRNA polyplex.
4
Polyplex Characterization by Particle Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The particle size and zeta potential of PDAPEI/pDNA polyplexes were measured at different w/w ratios by Particle-Size Analyzer (Brookhaven Instruments) and Zeta Potential Analyzer (Zetasizer Nano, Malvern Instruments), respectively at 25 °C. The morphology of PDAPEI/pDNA polyplexes was imaged by transmission electron microscopy (JEOL JEM 2010 system) at the w/w ratio of 2.
5
Transmission Electron Microscopy of Polyplexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Morphology of polyplexes was observed by Transmission Electron Microscopy (TEM, JEOL JEM 2010 system) under 120 kV. 10 μL polyplex solution was taken out carefully to be added on the copper net and then incubated overnight under room temperature. Polyplexes were observed by transmission electron microscopy (TEM) and images were recorded for further analysis.
6
Characterization of PDAPEI/miR-221/222 Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PDAPEI/miR-221/222 complexes were prepared by adding different concentrations of PDAPEI (2 mg/mL) to the DNA solution (20 ng/μL, miR-221 and miR-222 of equal weight). In this study, we selected preset nitrogen-to-phosphor (N/P) trial ratios of PDAPEI/miR-221/222 and used PEI25kDa/miR-221/222 complexes as a control. The particle size and zeta potential of the polyplexes were measured, using Brookhaven Particle Size Analyzer (90 Plus). The morphology of the polyplexes was observed using a transmission electron microscope (TEM, JEM 2010 system JEOL, Japan). The condensation ability of PDAPEI/miR-221/222 complexes was evaluated by gel electrophoresis, stained with ethidium bromide for semiquantitative measurements, and detected using a Gel Doc EZ system (Bio-Rad, Hercules, CA, USA). The results were repeated three times and the densitometry was shown as mean ± standard deviation (SD).
7
TEM Sample Preparation Methods
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples for transmission electron microscopy (TEM) were prepared by mechanical polishing, followed by ion-thinning methods. Cross-sectional TEM images were obtained using a JEOL JEM-2010 system at 200 kV.
8
Polyethylenimine-Butyrate Polyplex Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PEI-Bu/siRNA polyplexes were prepared by adding the PEI-Bu solution into siRNA solution at polymer to siRNA N/P ratio of 3.84, 7.67, 23.01, 38.35, 76.7, 115.05 and 153.4 and incubated at room temperature for 30 min. The formed siRNA nanoparticles were loaded on a 3.0% agarose gel containing 0.5 μg/mL ethidium bromide and subjected to electrophoresis at 110 V for 40 min. The retardation of siRNA was visualized by UV illuminator to identify condensing efficiency. The particle size and distribution of the polyplex at different N/P ratio in water and saline were measured by using Brookhaven Particle Size Analyzer (90 Plus), the Zeta potential of the nanoparticles was determined by using the same instrument as well. The values of the particle sizes and Zeta potentials were calculated by four individual experiments. A transmission electron microscopic (TEM, JEM 2010 system JEOL, Japan) was used to observe the image of the PEI-Bu siRNA polyplex.
Saline and BSA solution were used to mimic the physiological conditions to indicate the stability of the particles. The PEI-Bu siRNA polyplex was prepared as described and incubated in saline and BSA solution at 37°C separately for 48 hours. Particle size was determined at 0, 1, 3, 6, 12, 24, 36, 48 hours to study the stability of the formed polyplexes.
Saline and BSA solution were used to mimic the physiological conditions to indicate the stability of the particles. The PEI-Bu siRNA polyplex was prepared as described and incubated in saline and BSA solution at 37°C separately for 48 hours. Particle size was determined at 0, 1, 3, 6, 12, 24, 36, 48 hours to study the stability of the formed polyplexes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!