Agilent 2200 bioanalyser

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 2200 Bioanalyzer is a compact, automated electrophoresis system designed for the analysis of DNA, RNA, and proteins. It uses microfluidic technology to separate and detect biomolecules in a simple, quick, and reproducible manner.

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2 protocols using agilent 2200 bioanalyser

RNA Extraction from Exosomes

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TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract high‐quality total RNA from the exosomes obtained from each specimen. A NanoDrop 2000 spectrophotometer (NanoDrop Technologies; Thermo Fisher Scientific, Inc.) was used to analyse the concentration and quality of RNA. An Agilent 2200 Bioanalyser (Agilent Technologies, Inc.) was used to assess the RNA content.

Wu C., Wang M., Huang Q., Guo Y., Gong H., Hu C, & Zhou L. (2021). Aberrant expression profiles and bioinformatic analysis of CAF‐derived exosomal miRNAs from three moderately differentiated supraglottic LSCC patients. Journal of Clinical Laboratory Analysis, 36(1), e24108.

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RNA Sequencing Library Preparation

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Total RNA of each sample was extracted with the Qiagen Rneasy Mini Kit and digested with Dnase I according to the manufacturer’s instructions (Qiagen, Hilden, Germany). The integrity and size distribution of the RNA were verified with Agilent 2200 Bioanalyser (Agilent Technologies., USA.). Samples with the RNA Integrity Number ≥8.0 were used for cDNA library construction. The cDNA libraries for single-ending sequencing were constructed using Ion Total RNA-Seq Kit v2.0 (Life Technologies, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. The cDNA libraries were then processed for the Proton Sequencing process according to the commercially available protocols. Samples were diluted and mixed, and the mixture was processed on a OneTouch 2 instrument (Life Technologies) and enriched on a OneTouch 2 ES station (Life Technologies) for preparing the template-positive Ion PI™ Ion Sphere™ Particles (Life Technologies) according to Ion PI™ Template OT2 200 Kit v2.0 (Life Technologies). After enrichment, the mixed template-positive Ion PI™ Ion Sphere™ Particles of samples was loaded on to 1 P1v2 Proton Chip (Life Technologies) and sequenced on Proton Sequencers according to Ion PI Sequencing 200 Kit v2.0 (Life Technologies).

Hu C., Li Q., Shen X., Quan S., Lin H., Duan L., Wang Y., Luo Q., Qu G., Han Q., Lu Y., Zhang D., Yuan Z, & Shi J. (2016). Characterization of factors underlying the metabolic shifts in developing kernels of colored maize. Scientific Reports, 6, 35479.

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