α spt16

Manufactured by Santa Cruz Biotechnology

α-SPT16 is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect the SPT16 protein, which is a component of the FACT (facilitates chromatin transcription) complex. The FACT complex plays a role in regulating chromatin structure and transcription.

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Lab products found in correlation

α γ h2ax, by Abcam (1 mentions) α flag m2, by Merck Group (1 mentions) α pcna, by Santa Cruz Biotechnology (1 mentions) Normal mouse igg, by Merck Group (1 mentions) α macroh2a1, by Merck Group (1 mentions) α phospho h2ax, by Merck Group (1 mentions) α h2ax, by Abcam (1 mentions) α h2az, by Abcam (1 mentions) α h2b, by Abcam (1 mentions) α brca1, by Santa Cruz Biotechnology (1 mentions) α gapdh, by Santa Cruz Biotechnology (1 mentions) αh3k27ac, by Active Motif (1 mentions) α flag m2 affinity gel, by Merck Group (1 mentions) α flag, by Merck Group (1 mentions) α γh2ax, by Merck Group (1 mentions) α h2a, by Abcam (1 mentions) α h3k9me3, by Abcam (1 mentions) α hsc70, by Santa Cruz Biotechnology (1 mentions) α h3k4me3, by Active Motif (1 mentions)

2 protocols using α spt16

Chromatin Immunoprecipitation (ChIP) Assay

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The following Abs were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-macroH2A1 (Millipore 07–219), α-phospho-H2AX (Millipore 05–636), α-H2AX (Abcam ab20669), α-H2AZ (Abcam ab4174), α-H2B (Abcam ab52484), normal mouse IgG (Millipore 12–371). Antibodies for IP were α-Flag M2 (Sigma F1804) and α-BRCA1 (Santa Cruz sc-6954). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-PCNA (Santa Cruz sc-56), α-GAPDH (Santa Cruz sc-32233), α-SPT16 (Santa Cruz sc-28734), α-EZH2 (CST 5246s), α-SSRP1 (CST 134215), α-phospho-ATM (CST 4526s), α-phospho-CHK2 (CST 2661s), α-phospho-RPA S4/8 (Bethyl A300-245A), α-RPA2 (Millipore NA19L), α-H2AX (Abcam ab20669), α-H2AZ (Abcam ab4174), α-H2A (CST 12349s, Abcam ab15653). Secondary antibodies were goat anti-mouse IgG-HRP (Santa Cruz sc-2005, Invitrogen 31430) and goat anti-rabbit IgG-HRP (Santa Cruz sc-2004, Invitrogen 31460, CST 7074). Primary antibodies for IF were: α-γ-H2AX (Abcam ab11174, Milipore 05–636), α-BRCA1 (Santa Cruz, sc-6954), α-macroH2A1.2 (Millipore, MABE61), α-pRPA S33 (Bethyl, A300-246A), α-FANCD2 (Novus NB100-182). Secondary antibodies were from Life Technologies (goat-α-mouse or goat-α-rabbit conjugated to Alexa Fluor 488, 568, or 647).

Kim J., Sturgill D., Sebastian R., Khurana S., Tran A.D., Edwards G.B., Kruswick A., Burkett S., Hosogane E.K., Hannon W.W., Weyemi U., Bonner W.M., Luger K, & Oberdoerffer P. (2017). Replication stress shapes a protective chromatin environment across fragile genomic regions. Molecular cell, 69(1), 36-47.e7.

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Chromatin Profiling using CUT&RUN and Immunoblotting

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Antibodies used for CUT&RUN are αH2A (Abcam, 15653), αFLAG (Sigma, F1804), αmouse rb IgG (Jackson ImmunoResearch, 315-005-003). Primary antibodies used for immunoblotting include: αγH2Ax (Milipore, ab26350), αFLAG (Sigma, F7425), αH2B (CST, D2H6), αH4 (CST, D2X4V), αH3 (Immunoway, YM3038), αNAP1L1 (Abcam, ab33076), αNAP1L2 (Abnova, H00004674-D01), αSPT16 (Santa Cruz, sc-377028), αSSRP1 (Santa Cruz, sc-74536), αHSC70 (Santa Cruz, sc-7298), αC23 (Santa Cruz, sc-55486), αH2BK120ub (CST, D11), αH2AK119ub (CST, D11), αH3K4me3 (Active Motif, 61379), αH3K36me3 (Immunoway), αH3K36me2 (CST, 29015), αH3K9me3 (Abcam, ab8898), αH3K27me3 (CST, 9733), αH4K16ac (Immunoway, YK0014), αH4K20me3 (Active Motif, 39671), αH3K27ac (Active Motif, 39685), αH3K79me3 (Immunoway, YM3091), αH3K9ac (Immunoway, YK0006), αHA (Santa Cruz, SC7392), αβ-Actin (Immunoway, YM3028). αFLAG M2 Affinity Gel (Sigma), αH2A (Abcam, 15653), and αRpb1 (CST, 14958) were used for ChIP.

Kang T.Z., Zhu L., Yang D., Ding D., Zhu X., Wan Y.C., Liu J., Ramakrishnan S., Chan L.L., Chan S.Y., Wang X., Gan H., Han J., Ishibashi T., Li Q, & Chan K.M. (2021). The elevated transcription of ADAM19 by the oncohistone H2BE76K contributes to oncogenic properties in breast cancer. The Journal of Biological Chemistry, 296, 100374.

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