Protein levels of GFP, dnRhoA, and β-Actin were assessed using Western Blot, following previously described protocols.27 (link) Briefly, 5 μg of protein were separated on pre-cast SDS-PAGE gels (Bio-Rad) and transferred onto Immobilon IP membranes (Millipore) before probing for GFP (1:2500, Abcam, #ab6556), RhoA (1:500, #MA1-134, Thermo Fisher Scientific), β-Actin (1:2500, #ab170325, Abcam), and GAPDH (1:50,000, #PA1-9046, Thermo Fisher Scientific). Primary antibodies were detected using IRDye 800CW goat anti-mouse (#925-332210), IRDye 680RD donkey anti-rabbit (#925-68073), or IRDye 800CW donkey anti-goat (#926-32214) secondary antibodies (1:15,000, LI-COR). Blots were visualized on an Odyssey Infrared Imaging System (LI-COR). Blots were stripped with NewBlot PVDF Stripping Buffer (LI-COR) and reprobed once to allow for detection of all four proteins on the same blot.
Ab6556
Manufactured by Thermo Fisher Scientific
The Ab6556 is a laboratory instrument designed for the purpose of conducting scientific analysis. It is a versatile piece of equipment that can be used in a variety of research and testing applications. The core function of the Ab6556 is to perform measurements and data collection, though the specific intended use may vary depending on the needs of the user.
Automatically generated - may contain errors
Lab products found in correlation
Ab170325, by Abcam (1 mentions)
Irdye 680rd donkey anti rabbit, by LI COR (1 mentions)
Irdye 800cw donkey anti goat, by LI COR (1 mentions)
Newblot pvdf stripping buffer, by LI COR (1 mentions)
Irdye 800cw goat anti mouse, by LI COR (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Sds page gel, by Bio-Rad (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Odyssey clx system, by LI COR (1 mentions)
Ab182858, by Abcam (1 mentions)
Rm 9106, by Abcam (1 mentions)
3 protocols using ab6556
1
Quantifying Protein Expression by Western Blot
2
Quantitative Immunoblotting of GFP Variants
3
Immunohistochemical Analysis of DLBCL
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sections of paraffin-embedded formalin-fixed spleens (4 µm) were deparaffinized and stained with H&E or with specific antibodies to BCL2 (Abcam ab182858, 1:8000), GFP (Abcam ab6556, 1:1000), Ki-67 (Thermo Scientific RM9106, 1:100), or MYC (Abcam ab51154, 1:50). Of note, BCL2 antibody was able to detect both human and murine BCL2 as reported. Secondary antibodies against primary Fc were conjugated with horseradish peroxidase enzyme and developed by DAB color substrates, followed by counterstaining with hematoxylin. All slides were viewed under a light microscope and scanned using either a Leica Aperio CS2 whole-slide scanner at ×20 magnification (for an expert pathologist evaluation and confirmation of the high grade DLBCL phenotype), or a Nikon NIS Elements Microscope Imaging at ×20 and ×100 magnification (for visualization of tumors at a greater resolution and magnification).
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