Digoxigenin RNA probes were synthesized using the T3/T7 Transcription Kit (Roche, Merk, Rahway, USA; Table S2 ). Larvae were collected, euthanized in MS222 at 200 mg/L and fixed 12 h in 4%. paraformaldehyde diluted in PBS (phosphate-buffered saline). Samples were subsequently dehydrated stepwise in PBS/ethanol, and then put three times 10 min in butanol 100% and finally in two bath of paraffin (respectively 1 and 4 h) before being embedded in blocks. Embedded larvae were sectioned transversally at 7 μm using a Leica Biosystems RM2245 Microtome the day before starting in situ hybridization (Figure 2C ). The samples were then treated as in Thisse et al.68 (link)
T3 t7 transcription kit
Manufactured by Roche
Sourced in United States
The T3/T7 Transcription Kit is a laboratory tool used for in vitro transcription. It provides the necessary components, including RNA polymerase enzymes and ribonucleotides, to facilitate the synthesis of RNA from DNA templates.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using t3 t7 transcription kit
1
In Situ Hybridization of Digoxigenin RNA Probes
2
In Situ Hybridization of Digoxigenin RNA Probes
3
Digoxigenin RNA In Situ Hybridization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Digoxigenin RNA probes were synthesized using the T3/T7 Transcription Kit (Roche; Supp. Table 1).
Larvae were collected, euthanized in MS222 at 200 mg/L and fixed 12 hours in 4%. paraformaldehyde diluted in PBS (phosphate-buffered saline). Samples were subsequently dehydrated stepwise in PBS/ethanol, and then put three times 10 min in butanol 100% and finally in two bath of paraffin (respectively 1 and 4 hours) before being embedded in block. Embedded larvae were sectioned transversally at 7µm using Leica Biosystems RM2245 Microtome the day before starting ISH. The samples were then treated as in Thisse, Thisse, Schilling, and Postlethwait (1993) .
Larvae were collected, euthanized in MS222 at 200 mg/L and fixed 12 hours in 4%. paraformaldehyde diluted in PBS (phosphate-buffered saline). Samples were subsequently dehydrated stepwise in PBS/ethanol, and then put three times 10 min in butanol 100% and finally in two bath of paraffin (respectively 1 and 4 hours) before being embedded in block. Embedded larvae were sectioned transversally at 7µm using Leica Biosystems RM2245 Microtome the day before starting ISH. The samples were then treated as in Thisse, Thisse, Schilling, and Postlethwait (1993) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!