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Mar1 5a3 ifnar neutralizing antibody

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MAR1–5A3 is an interfereron alpha/beta receptor (IFNAR) neutralizing antibody. It binds to the IFNAR and blocks the type I interferon signaling pathway.

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MAR1-5A3 (25 citations)

4 protocols using mar1 5a3 ifnar neutralizing antibody

1

Myocardial Infarction in Mice

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Mice were intubated and ventilated with 2% isoflurane. After exposing the heart via thoracotomy at the fourth left intercostal space, the left coronary artery was permanently ligated with an 8–0 nylon monofilament suture. The thorax was closed with a 5–0 suture. Mice were treated with buprenorphine for analgesia on the day of surgery and twice daily thereafter for 72 hours. For cardioprotection experiments, mice were treated with two intraperitoneal doses of 500 μg of MAR1–5A3 IFNAR neutralizing antibody (BioXCell) at 8–12 hours and at 48 hours after permanent coronary ligation. Surgeries were performed in a blinded fashion unless genotype was obviated by unavoidable ascertainment of features such as coat color.
King K.R., Aguirre A.D., Ye Y.X., Sun Y., Roh J.D., Ng RP J.r., Kohler R.H., Arlauckas S.P., Iwamoto Y., Savol A., Sadreyev R.I., Kelly M., Fitzgibbons T.P., Fitzgerald K.A., Mitchison T., Libby P., Nahrendorf M, & Weissleder R. (2017). IRF3 and Type I Interferons Fuel a Fatal Response to Myocardial Infarction. Nature medicine, 23(12), 1481-1487.
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2

SARS-CoV-2 Infection Study in K18-hACE2 Mice

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All the B6/JGpt-H11em1Cin(K18-ACE2)/Gpt mice (K18-hACE2 KI mice, heterozygote, #T037657) were obtained from GemPharmatech Co., Ltd. (Nanjing, China) and were maintained in individually ventilated cages (IVCs) in ABSL-II (AUP # SKLV-AE2023-007). The 6- to 8-week-old mice were infected via intranasal route with indicated Lentivirus, and then were weighed and monitored daily. In subsequent experiments, 14 days after Lentivirus-N transduction, mice were intranasally inoculated with 4 × 106 TCID50 of SARS-CoV-2 trVLPs, and the weights of mice were monitored daily. Mice were sacrificed at 2, 4, 6, 9 dpi for tissue processing. For experiments of IFNAR neutralizing antibody treatment, mice received two intraperitoneal injections of 1 mg of MAR1–5A3 IFNAR neutralizing antibody (BioXCell), both 24 h before and 24 h after trVLPs infection. All experiments were made to minimize animal suffering and have been approved by an ethical committee.
Deng J., Yang S., Li Y., Tan X., Liu J., Yu Y., Ding Q., Fan C., Wang H., Chen X., Liu Q., Guo X., Gong F., Zhou L, & Chen Y. (2024). Natural evidence of coronaviral 2′-O-methyltransferase activity affecting viral pathogenesis via improved substrate RNA binding. Signal Transduction and Targeted Therapy, 9, 140.
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3

Myocardial Infarction in Mice

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Mice were intubated and ventilated with 2% isoflurane. After exposing the heart via thoracotomy at the fourth left intercostal space, the left coronary artery was permanently ligated with an 8–0 nylon monofilament suture. The thorax was closed with a 5–0 suture. Mice were treated with buprenorphine for analgesia on the day of surgery and twice daily thereafter for 72 hours. For cardioprotection experiments, mice were treated with two intraperitoneal doses of 500 μg of MAR1–5A3 IFNAR neutralizing antibody (BioXCell) at 8–12 hours and at 48 hours after permanent coronary ligation. Surgeries were performed in a blinded fashion unless genotype was obviated by unavoidable ascertainment of features such as coat color.
King K.R., Aguirre A.D., Ye Y.X., Sun Y., Roh J.D., Ng RP J.r., Kohler R.H., Arlauckas S.P., Iwamoto Y., Savol A., Sadreyev R.I., Kelly M., Fitzgibbons T.P., Fitzgerald K.A., Mitchison T., Libby P., Nahrendorf M, & Weissleder R. (2017). IRF3 and Type I Interferons Fuel a Fatal Response to Myocardial Infarction. Nature medicine, 23(12), 1481-1487.
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4

Myocardial Infarction in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were intubated and ventilated with 2% isoflurane. After exposing the heart via thoracotomy at the fourth left intercostal space, the left coronary artery was permanently ligated with an 8–0 nylon monofilament suture. The thorax was closed with a 5–0 suture. Mice were treated with buprenorphine for analgesia on the day of surgery and twice daily thereafter for 72 hours. For cardioprotection experiments, mice were treated with two intraperitoneal doses of 500 μg of MAR1–5A3 IFNAR neutralizing antibody (BioXCell) at 8–12 hours and at 48 hours after permanent coronary ligation. Surgeries were performed in a blinded fashion unless genotype was obviated by unavoidable ascertainment of features such as coat color.
Calcagno D.M., Ng RP J.r., Toomu A., Zhang C., Huang K., Aguirre A.D., Weissleder R., Daniels L.B., Fu Z, & King K.R. (2020). The myeloid type I interferon response to myocardial infarction begins in bone marrow and is regulated by Nrf2-activated macrophages. Science immunology, 5(51), eaaz1974.
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