Breast cancer cell lines BT-474, MCF7, Hs-578-T, MDA-MB-231, MDA-MB-453, and MDA-MB-468 (Cobioer, Nanjing, China) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sunncell, Wuhan, China) with 10% fetal bovine serum (FBS) and certain cell growth factor. BT-549, HCC1937, and T47D cell lines were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Sunncell, Wuhan, China) with 10% or 20% FBS.
For the following function experiments, T47D, MCF7, MDA-MB-453, HCC1937, MDA-MB-231, and MDA-MB-468 cells were treated with 100 or 500 nM GDC-0941 (PI3K selective inhibitor, MedChemExpress, New Jersey, USA) or dimethyl sulfoxide (DMSO) for 24 h. In addition, MDA-MB-231 and HCC1937 cells were treated with 100 nM OSU-T315 (ILK inhibitor, SolelyBio, Beijing, China), tumor necrosis factor (TNF)-α, IgG, or TNF-α antibody (ab6671, Abcam, Cambridge, UK).
In addition, MCF7 and MDA-MB-453 cells were transfected with ILK, and MDA-MB-231 and HCC1937 cells were transfected with shILK.
For the following function experiments, T47D, MCF7, MDA-MB-453, HCC1937, MDA-MB-231, and MDA-MB-468 cells were treated with 100 or 500 nM GDC-0941 (PI3K selective inhibitor, MedChemExpress, New Jersey, USA) or dimethyl sulfoxide (DMSO) for 24 h. In addition, MDA-MB-231 and HCC1937 cells were treated with 100 nM OSU-T315 (ILK inhibitor, SolelyBio, Beijing, China), tumor necrosis factor (TNF)-α, IgG, or TNF-α antibody (ab6671, Abcam, Cambridge, UK).
In addition, MCF7 and MDA-MB-453 cells were transfected with ILK, and MDA-MB-231 and HCC1937 cells were transfected with shILK.