Ab214369

Manufactured by Abcam

Sourced in United Kingdom

Ab214369 is a laboratory equipment product. It serves a core function related to scientific research and analysis, but a detailed description cannot be provided while maintaining an unbiased and purely factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab125213, by Abcam (1 mentions) Ab53154, by Abcam (1 mentions) Ab59348, by Abcam (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Sc 398631, by Santa Cruz Biotechnology (1 mentions) Ab34710, by Abcam (1 mentions) A21058, by Thermo Fisher Scientific (1 mentions) A21076, by Thermo Fisher Scientific (1 mentions) Mab374, by Merck Group (1 mentions) Ab59469, by Abcam (1 mentions) D9542, by Merck Group (1 mentions) Tcs sp8, by Leica (1 mentions)

3 protocols using ab214369

Apelin, APJ, Bcl-2, and Bax Expression in Cardiac Tissue

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Western blotting was used to measure the apelin, APJ, Bcl-2, and Bax expression levels in the left ventricular tissues. The frozen tissues were weighed and homogenized in RIPA lysis buffer. Protein levels in the supernatant were determined using the Bradford method (21 (link)). Thereafter, 40 µg of protein was subjected to SDS-PAGE with 10% separation and transferred onto a nitrocellulose membrane for 3 h at 200 mA. The membranes were blocked with 1% bovine serum albumin for 1 h. Subsequently, primary antibodies for apelin (ab125213, dilution, 1:500; Abcam,
Cambridge, United Kingdom), APJ (ab214369, dilution, 1:500; Abcam, Cambridge, United Kingdom), Bcl-2 (ab59348, dilution, 1:500; Abcam, Cambridge, United Kingdom), and Bax (ab53154, dilution, 1:500; Abcam, Cambridge, United Kingdom) were added, followed by overnight incubation at 4°C. After washing twice with TBS, membranes were incubated with the respective secondary antibodies (dilution, 1:10,000; Santa Cruz, CA, USA). Finally, protein band intensities were quantified using a densitometer analysis system (Quantity One software, Bio-Rad, PA, USA).

Bi F., Xu Y, & Sun Q. (2018). Catalpol pretreatment attenuates cardiac dysfunction following myocardial infarction in rats. Anatolian Journal of Cardiology, 19(5), 296-302.

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Protein Expression Analysis by Western Blot

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Western blotting was performed as previously described [23] . Primary antibodies used were as collagen 1 (ab34710, Abcam, 1:500), periostin (sc-398,631, Santa Cruz, 1:500), apelin receptor (ab214369, Abcam, 1:500) and GAPDH (MAB374, Merck, 1:100,000). Secondary antibodies were from Life Technologies (Alexa Fluor A11371, A21058, and A21076) with 1:5000 dilution. Quantifications are shown as relative to loading control (GAPDH).

Lin R., Rahtu-Korpela L., Szabo Z., Kemppi A., Skarp S., Kiviniemi A.M., Lepojärvi E.S., Halmetoja E., Kilpiö T., Porvari K., Pakanen L., Tolva J., Paakkanen R., Segersvärd H., Tikkanen I., Laine M., Sinisalo J., Lakkisto P., Huikuri H., Magga J., Junttila J, & Kerkelä R. (2022). MiR-185-5p regulates the development of myocardial fibrosis. Journal of molecular and cellular cardiology, 165.

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Immunofluorescence Staining for Apelin and APJ

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All specimens were fixed with 4% paraformaldehyde (PFA), washed with PBS, permeabilized with 1% Triton-X 100 in PBS for 10 min, blocked with 1% BSA for 1 h at room temperature, and then incubated with primary antibodies overnight at 4℃. Specimens were then incubated with the fluorescent secondary antibodies and DAPI J o u r n a l P r e -p r o o f
(1:1000, D9542, Sigma) for 1 h in the dark at room temperature. The specimens were analyzed using a confocal microscope (LEICA TCS SP8). The primary antibodies used in immunofluorescence staining were as the follows: rabbit anti-Apelin (1:800, ab59469, Abcam), rabbit anti-APJ (1:800, ab214369, Abcam), mouse anti-myosin7α
(1:1000, DSHB), and rabbit anti-cleaved caspase-3 (1:1000, 9664S, Cell Signaling Technology).

Yin H., Zhang H., Kong Y., Wang C., Guo Y., Gao Y., Yuan L., Yang X, & Chen J. (2020). Apelin protects auditory cells from cisplatin-induced toxicity in vitro by inhibiting ROS and apoptosis. Neuroscience letters, 728.

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