Chicken ovalbumin ova

Manufactured by Merck Group

Sourced in United States

Chicken ovalbumin (OVA) is a laboratory-grade protein derived from egg whites. It is a widely used standard and control substance in various biochemical and immunological assays. The core function of OVA is to serve as a reference material for quantification, standardization, and quality control purposes in research and diagnostic applications.

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Lab products found in correlation

Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (3 mentions) Limulus amebocyte lysate assay kit, by Lonza (1 mentions) Rabbit anti mouse cox 2, by Abcam (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions) Detoxi gel endotoxin removing gel, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Aluminium hydroxide, by Merck Group (1 mentions) Naive cd4 isolation kit, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

OVA (1263 citations) Chicken ovalbumin (27 citations) Chicken OVA (18 citations) Chicken egg ovalbumin (OVA; grade V (5 citations) Chicken egg ovalbumin (OVA) (3 citations) Whole chicken ovalbumin (OVA) (2 citations) Chicken ovalbumin (OVA; Grade V; (2 citations)

5 protocols using chicken ovalbumin ova

Optimizing In Vivo Experiments

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GR was obtained from Korea Ginseng Corporation (Seoul, Republic of Korea) and GB was provided from R&D Center, Amorepacific Corporation as described previously (Gyeongi-do, Republic of Korea) [15 (link)]. Chicken ovalbumin (OVA) was obtained from Sigma-Aldrich. The endotoxin levels contained in the amount of GR and GB (50 mg/kg) and OVA (50 μg) used in each in vivo experiment were evaluated using a Limulus amebocyte lysate (LAL) assay kit (Lonza) and were less than 0.1 endotoxin unit/ml.

Zhang W., Cho S.Y., Xiang G., Min K.J., Yu Q, & Jin J.O. (2015). Ginseng Berry Extract Promotes Maturation of Mouse Dendritic Cells. PLoS ONE, 10(6), e0130926.

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Immunophenotyping of Dendritic Cells

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Chicken ovalbumin (OVA) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Mouse fluorescein-conjugated antibodies against CD11c, I-A^b, CD80, CD86, CD40, and FcγRIIB were purchased from eBioscience. Enzyme-linked immunosorbent assay (ELISA) kits for the detection of mouse interleukin (IL)-12, IL-6, IL-4, and IL-13 as well as an enzyme immunoassay (EIA) kit for evaluation of prostaglandin E2 (PGE2) were bought from R&D Systems (Minneapolis, MN, USA).Rabbit anti-mouse Cox-2 and horseradish peroxidase (HRP)-conjugated anti-rabbit Ig secondary antibodies were purchased from Abcam (Cambridge, UK).

Zhu T., Chen R., Li Z., Tian J., Deng C., Zhang X., Zhang K., Tong L., Yu Y, & Bai C. (2016). Functional Role of FcγRIIB in the Regulation of Mesenchymal Stem Cell Function. International Journal of Medical Sciences, 13(2), 154-160.

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Purification and Endotoxin Removal of G. formosanum Polysaccharide

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The major polysaccharide fraction PS-F2 was purified from the submerged culture of G. formosanum Chang et Chen (ATCC 76538) as previously described (Wang et al. 2011 (link)). The purified PS-F2 was passed through an endotoxin removal column (Detoxi-Gel Endotoxin Removing Gel, Thermo Scientific, Rockford, IL) and the endotoxin level in the samples was determined to be < 0.3 EU/mg by the Pyrotell Limulus Amebocytes Lysate (LAL) test (Associates of Cape Cod, Falmouth, MA). Chicken ovalbumin (OVA) and pentobarbital sodium were purchased from Sigma-Aldrich (St. Louis, MO) and passed though the Detoxi-Gel Endotoxin Removing Gel before use. Hanks’ balanced salt solution (HBSS) was purchased from Thermo Scientific HyClone (Logan, UT). Fetal bovine serum (FBS) was purchased from Biological Industries (Beit-Haemek, Israel). Dulbecco's phosphate buffered saline (DPBS) was purchased from Life Technologies (Gaithersburg, MD). All other chemicals were purchased from commercial sources at the highest purity available.

Pi C.C., Wang H.Y., Lu C.Y., Lu F.L, & Chen C.J. (2014). Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma. SpringerPlus, 3, 297.

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Induction of Allergic Airway Inflammation

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We followed our previously established protocol to induce acute allergic airway inflammation with OVA with minor changes [15 (link)]. Briefly, mice were sensitized to 8 μg chicken ovalbumin (OVA; Sigma-Aldrich, St. Louis, MO, USA) bound to 4 mg aluminium hydroxide (Sigma-Aldrich) in phosphate-buffered saline intraperitoneally (i.p.) at day 1 and 6. On days 14–18, the mice were challenged once a day intranasally (i.n.) with 100 μg OVA in 25 μL PBS to induce allergic airway inflammation (12 OVA/OVA mice). The control group was exposed to PBS only during these 5 days (10 OVA/PBS mice).

Lässer C., Kishino Y., Park K.S., Shelke G.V., Karimi N., Suzuki S., Hovhannisyan L., Rådinger M, & Lötvall J. (2021). Immune-Associated Proteins Are Enriched in Lung Tissue-Derived Extracellular Vesicles during Allergen-Induced Eosinophilic Airway Inflammation. International Journal of Molecular Sciences, 22(9), 4718.

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Tracking T Cell Responses with Radiolabeled Antibodies

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Singe cell suspensions of CD4⁺ T cells (from OT-II donor mice) and CD8⁺ T cells (from OT-I donor mice) were prepared from spleen and lymph nodes using the naive CD4⁺ isolation kit and the CD8a⁺ isolation kit, respectively, according to the manufacturer’s instructions (Miltenyi Biotec). The purity of the isolated cells was >95 % as determined by cytofluorimetry. Pooled cells were washed and resuspended in PBS, followed by retro-orbital injection of 2 x 10⁶ CD4⁺ and/or CD8a⁺ cells into RAG1^−/− recipients. On day 1 after cell transfer, mice received 100 μg of chicken ovalbumin (OVA) (Sigma-Aldrich), emulsified in complete Freund’s adjuvant (CFA) (1:1 ratio), by subcutaneous (s.c.) injection in the scruff of the neck. Immunized mice received 50 μCi (1850 kBq) radiolabeled anti-CD4 scFv or anti-CD8 VHH by retro-orbital injection on day 4 and 11. PET-CT images were then acquired on days 5 and 12 (Fig. 3A).

Islam A., Pishesha N., Harmand T.J., Heston H., Woodham A.W., Cheloha R.W., Bousbaine D., Rashidian M, & Ploegh H.L. (2021). Converting an anti-mouse CD4 monoclonal antibody into an scFv PET imaging agent for longitudinal monitoring of CD4+ T cells. Journal of immunology (Baltimore, Md. : 1950), 207(5), 1468-1477.

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