Ab223694
Manufactured by Abcam
Sourced in United Kingdom
Ab223694 is a primary antibody that specifically recognizes the target antigen. It is intended for use in various immunoassay applications to detect the presence and quantity of the target protein.
Automatically generated - may contain errors
Lab products found in correlation
Ab2792, by Abcam (2 mentions)
Alexa fluor 488 affinipure goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions)
Confocal laser scanning microscope, by Zeiss (1 mentions)
Alexa fluor 594 affinipure goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions)
Ab219800, by Abcam (1 mentions)
Ab214185, by Abcam (1 mentions)
Ab175449, by Abcam (1 mentions)
Ab234437, by Abcam (1 mentions)
Ab191860, by Abcam (1 mentions)
Ab133514, by Abcam (1 mentions)
Pa5 99390, by Thermo Fisher Scientific (1 mentions)
Ab6728, by Abcam (1 mentions)
Ab176921, by Abcam (1 mentions)
Dab abc detection ihc kit, by Abcam (1 mentions)
4 protocols using ab223694
1
Immunohistochemical Staining of WHSC1 and P4HB
2
Immunofluorescence Analysis of WHSC1 and P4HB
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Cells were seeded on slides, at a density of 2 × 104 cells/well, and incubated overnight. Following incubation, the cells were washed with phosphate buffer saline (PBS), fixed with 4% paraformaldehyde solution, permeabilized with 0.1% Triton X, and blocked with bovine serum albumin (BSA) for 1 h. The cells were then incubated with WHSC1 (1:100, ab223694; Abcam, Cambridge, UK) and P4HB (1:100, ab2792; Abcam, Cambridge, UK) primary antibodies at 4°C overnight. The slides were then washed with PBS and treated with Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson, Carlsbad, USA), and Alexa Fluor® 594 AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson, Carlsbad, USA) secondary antibodies. The slides were then placed under a confocal laser scanning microscope (ZEISS, Germany) for the immunofluorescence study. The magnification used in our study was 10X and the sampling size was XY. The color Red was generated with a 532 nm laser and LP550 filter; Green with a 488 nm laser and LP505 filter; and Blue with a mercury lamp and BP415-480 filter. We used the Pearson coefficient analysis for our study.
3
Western Blot Analysis of NLRP3 Inflammasome
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Lysates from tissues and cells were prepared using RIPA lysis buffer and were quantified using a BCA kit (cat. #BCA1; Sigma–Aldrich). Afterwards, lysates were resolved in SDS-PAGE to separate proteins and were then transferred to polyvinylidene fluoride membranes. The membranes were immersed in 5% skimmed milk to block for non-specific binding and labelled with anti-WHSC1 (1:2000; cat. #ab223694; Abcam), NLRP3 (1:500; cat. #ab214185; Abcam), ASC (1:2000; cat. #ab175449; Abcam), caspase-1 p20 (1:1000; cat. #PA5-99390; Thermo Fisher Scientific), IL-18 (1:1000; cat. #ab191860; Abcam), IL-1β (1:1000; cat. #ab234437; Abcam), GSDMD FL (full length GSDMD; 1:1000; cat. #ab219800; Abcam), GSDMD CL (GSDMD-N terminal segment; 1:1000; cat. #10137S; Cell Signaling Technology, Danvers, MA), NEK7 (1:3000; cat. #ab133514; Abcam) and β-actin (1:5000; cat. #ab8227; Abcam) Abs at 4°C for 12 h. Afterwards, the membranes were incubated with IgG H&L (HRP; 1:5000; cat. #ab6728; Abcam) for 2 h at room temperature. β-Actin was designated as the internal control protein. Densitometry of bands was measured utilising the ECL Western Blotting Substrate (cat. #32132X3; Thermo Fisher Scientific).
4
Endometrial NSD2 and H3K36me2 in Recurrent Implantation Failure
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Proliferative-phase endometrial tissues from 10 patients with RIF and 10 FER controls were collected and washed with PBS immediately. Tissues were fixed in 4% paraformaldehyde, paraffin-embedded, and serially sectioned. Immunostaining was performed according to the instruction of the mouse and rabbit specific HRP/diaminobenzidine (DAB) (ABC) Detection IHC Kit (ab64264, Abcam). The slides were incubated with primary antibody against NSD2 (1:100, ab223694, Abcam), H3K36me2 (1:1000, ab176921, Abcam), minichromosome maintenance complex component 7 (MCM7) (1:500, 11225-1-AP, Proteintech), or Rabbit IgG (1:500, #3900, Cell Signaling Technology) diluted in PBS overnight at 4°C. The primary antibodies used in the study were all commercial antibodies reported by other empirical studies (Zhu et al. 2019 (link), Dai et al. 2020 (link), Ganig et al. 2021 (link), Acke et al. 2022 (link)). For each immunohistochemical run, a slice from each group was randomly selected as the negative control. The negative control was tested by incubation with immunoglobulin G (IgG) from the corresponding species. Next day, they were incubated with secondary antibodies, stained with DAB, and counterstained with hematoxylin. The slides were then dehydrated, cleared, and mounted. The images were captured under a microscope. See Supplementary methods for the immunohistochemical scoring method.
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