RAW264.7 cells (ATCC, Manassas, VA) were grown in Dulbecco's modified Eagle's medium (DMEM; Life Technologies, Grand Island, NY) with 10% fetal calf serum (FCS). In some experiments, we added recombinant TGF-β2 (rTGF-β2; 0.5-1 ng/mL; R&D, Minneapolis, MN) or E. coli O111:B4 lipopolysaccharide (LPS; 0.5μg/mL; Sigma).
Lipopolysaccharide lps e coli o111 b4
Manufactured by Merck Group
Sourced in United States
Lipopolysaccharide (LPS) (E. coli O111:B4) is a laboratory product derived from the cell wall of the Escherichia coli O111:B4 bacterial strain. LPS is a key component of the outer membrane of Gram-negative bacteria and is commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dimyristoyl sn glycero 3 phosphocholine, by NOF corporation (3 mentions)
1 2 dipalmitoyl sn glycero 3 phosphocholine, by NOF corporation (3 mentions)
1 2 distearoyl sn glycero 3 phosphocholine, by NOF corporation (3 mentions)
Rtgf β2, by R&D Systems (2 mentions)
Raw 264.7 cells, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Wako cholesterol e kit, by Fujifilm (1 mentions)
Fk866, by Beyotime (1 mentions)
Dextran sulfate, by Merck Group (1 mentions)
Recombinant mouse il 4, by Thermo Fisher Scientific (1 mentions)
5 protocols using lipopolysaccharide lps e coli o111 b4
1
Exploring TGF-β and LPS Effects on RAW264.7 Cells
2
Exploring TGF-β and LPS Effects on RAW264.7 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RAW264.7 cells (ATCC, Manassas, VA) were grown in Dulbecco's modified Eagle's medium (DMEM; Life Technologies, Grand Island, NY) with 10% fetal calf serum (FCS). In some experiments, we added recombinant TGF-β2 (rTGF-β2; 0.5-1 ng/mL; R&D, Minneapolis, MN) or E. coli O111:B4 lipopolysaccharide (LPS; 0.5μg/mL; Sigma).
3
Lipid Compounds for Biomembrane Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The compounds 1-palmitoyl-2-oleoyl-glycero-3phosphocholine (POPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) were purchased from NOF corporation (White Plains, NY, USA). The compound 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[azido(polyethylene glycol)-2000] (DSPE-PEG2k) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Lipopolysaccharide (LPS) (E. coli O111:B4) was purchased from Sigma Aldrich (St. Louis, MO, USA). Cholesterol was purchased from Sigma Aldrich (Milwaukee, WI, USA). Wako Cholesterol E Kit was purchased from Fujifilm (Richmond, VA, USA). IL-6 and TNF-α ELISA kits were purchased from Invitrogen (Ann Arbor, MI, USA).
4
Visfatin Regulation in LPS-Induced Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lipopolysaccharide (LPS) E.coli O111:B4 was obtained from Sigma Aldrich (St. Louis, MO, USA). Recombinant mouse visfatin was obtained from Prospec (cyt-447, Prospec, Germany). FK866 was procured from Beyotime Institute of Biotechnology (Shanghai, China). The antibody of visfatin for immunohistochemistry (IHC) was obtained from ABClonal (Wuhan, China). Horseradish peroxidase (HRP) Conjugated Goat Anti-Mouse IgG was purchased from Yifeixue Bio Tech (Nanjing, China).
5
Polyphosphates Modulate Innate Immune Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
If not stated otherwise, the following concentrations of reagents were used: 100 ng/ml lipopolysaccharide (LPS; E. coli O111:B4, Sigma-Aldrich, St. Louis, MO), 10 μg/ml Zymosan (ZYM; S. cerevisiae, InvivoGen, San Diego, CA), 10 μg/ml Peptidoglycan (PGN; E. coli O111:B4, InvivoGen), 10 μg/ml Flagellin (FLA, B. subtilis, InvivoGen), 1 μg/ml dsDNA (E. coli K12, InvivoGen) transfected using Oligofectamine, 500 U/ml recombinant mouse IFNβ (Pestka Biomedical Laboratories), 10 ng/ml recombinant mouse IL-4 (Peprotech), and 100 μg/ml dextran sulfate (Sigma-Aldrich). S-polyphosphates had a modal length of 70 phosphates (range: 25–125 phosphates) and L-polyphosphates (unmodified or biotinylated) had a modal length of 700 phosphates (range: 200–1300 phosphates) and were solubilized from high-molecular-weight polyphosphate as described before64 (link). S-/L-polyphosphates were used as 50 µM (indicated as concentration of monophosphates) unless stated otherwise in the figures.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!