The largest database of trusted experimental protocols

Acrylamide

Manufactured by Solarbio
Sourced in China

Acrylamide is a chemical compound commonly used in laboratory equipment and procedures. It is a colorless, odorless, and crystalline solid that serves as a key component in various analytical and experimental processes. The core function of acrylamide is to facilitate the formation of polyacrylamide gels, which are widely used in techniques such as electrophoresis for the separation and analysis of proteins, nucleic acids, and other biomolecules.

Automatically generated - may contain errors

3 protocols using acrylamide

1

Comprehensive Protocol for Microbial Analyses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pro and Pep were purchased from the Nutrily Biotechnology, Ltd. (Anyang, Henan, China). Lactobacillus acidophilus JCM 1132 was purchased from the China General Microbiological Culture Collection Center (Beijing, China). Pepsin and trypsin were purchased from the Novozymes (Beijing, China). Bicinchoninic acid (BCA) protein assay kit, acrylamide, glycine, sodium dodecyl sulfate (SDS), Tris, urea and loading buffer were purchased from Solarbio (Beijing, China). Ammonium persulfate and tetramethylethylenediamine (TEMED) were purchased from Amersco (Framingham, USA). Coomassie Brilliant Blue R-250 was purchased from Bio-Rad (Richmond, CA, USA). MRS broth and MRS agar were purchased from Aobox Biotechnology, Ltd. (Beijing, China). Bis trifluoroacetamide (BSTFA), fatty acid methyl ester (FAME), methoxy amination hydrochloride, trimethyl chlorosilane (TMCS), standards of lactic acid, acetic acid, glycine, Gly-Gly-Gly, bacitracin and insulin were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2-Chloro-l-phenylalanine was purchased from Hengbai Biological Technology, Ltd. (Shanghai, China). High performance liquid chromatography (HPLC) grades of acetonitrile, methanol, chloroform, pyridine and trifluoroacetic acid (TFA) were purchased from Fisher Scientific (Ottawa, ON, Canada).
+ Open protocol
+ Expand
2

Signaling Molecule Antibody Procurement

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospho-Cofilin and Phospho-stathmin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Cofilin and Stathmin antibodies were purchased from Proteintech (Chicago, USA). β-actin and α-tubulin antibodies were from Boster (Wuhan, China). HRP-labeled anti-rabbit and anti-mouse antibodies were purchased from Zsgb-Bio (Beijing, China).
Cytochalasin D and nocodazole were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Glutamate, hydrogen peroxide, jasplakinolide, and taxol were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Human vascular endothelial growth factor (VEGF) and histamine were obtained from Peprotech (Rocky Hill, NJ, USA). LPS, TNF-α, Ang-II, and Capsaicin were purchased from Absin (Shanghai, China). Acrylamide, Muscone, and α-Asarone were from Solarbio (Beijing, China).
+ Open protocol
+ Expand
3

Molecular Signaling Pathway Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospho-co lin and Phospho-stathmin-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Co lin and Stathmin antibodies were purchased form proteintech (Chicago, USA). βactin and α-tubulin antibodies were from Boster (Wuhan, China). HRP-labeled anti-rabbit and anti-mouse antibodies were purchased from Zsgb-Bio (Beijing, China).
Cytochalasin D and nocodazole were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Glutamate, hydrogen peroxide, jasplakinolide, and taxol were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Human vascular endothelial growth factor (VEGF) and histamine were obtained from Peprotech (Rocky Hill, NJ, USA). LPS, TNF-α, Ang-, and Capsaicin were purchased from Absin (Shanghai, China). Acrylamide, Muscone, and α-Asarone were from Solarbio (Beijing, China).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!