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Vamp7

Manufactured by Novus Biologicals

VAMP7 is a protein that functions as a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) involved in intracellular membrane fusion and trafficking processes. It is a member of the vesicle-associated membrane protein (VAMP) family.

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2 protocols using vamp7

1

Immunoblotting and Immunofluorescence Analysis of Extracellular Vesicle Proteins

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The antibodies used for immunoblotting and immunofluorescence were as follows: Cortactin (H222) (3503; Cell Signaling Technology), VAMP7 (NB100–91356; Novus Biologicals), RAB7 (D95F2) (9367 T; Cell Signaling Technology), RAB27B (13412–1-AP; Proteintech), CD63 (GTX28219; GeneTex), β-actin (20536–1-AP; Proteintech), TSG101(28283–1-AP; Proteintech), CD81(A5270; ABclonal), SNAP23 (ab4114; Abcam), HRP-conjugated anti-rabbit IgG (7074S; Cell Signaling Technology), and HRP-conjugated anti-mouse IgG (7076S; Cell Signaling Technology). The reagent used for immunoblotting was Phalloidin-iFluor 594 reagent (ab176757; Abcam).
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2

Immunohistochemical Analysis of Kidney Cellular Markers

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Cells were washed with PBS, fixed with 4% paraformaldehyde, permeabilized with PBS-0.2% Triton X 100, and blocked with 5% FBS. Immunostaining were performed using the following primary antibodies: Cleaved Caspase 3 (CST #9664 1:500), Fluorescein labeled LTL (Vector #FL-1321 1:500), AQP2 (Santa Cruz #sc-9882 1:200), Fluorescein labeled DBA (Vector #FL-1031-5 1:500), Fluorescein labeled PNA (Vector # FL-1071-5 1:500), DPEP1 (Invitrogen #PA5-52984 1:200), CHMP1A (Proteintech #15761-1-AP 1:200), EEA1 (BD#610456 1:200), RAB5 (CST #3547 1:200), RAB7 (Sigma #R8779 1:200), RAB11 (BD #610658 1:200), VAMP7 (NOVUS #NBP1-07118 1:200), and GM130 (BD #610822 1:200).
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