Whole blood was collected from 15 individual healthy male and female blood donors (36 ± 10 years old) after written informed consent was given. HAS was prepared by centrifuging whole blood onto 9 mL silicon coated blood collection tubes (VACUETTE® z serum clot activator, Greiner bio-one, Kremsmünster, Austria) at 1770 g for 10 min. The top layer containing the supernatant was removed and the resulting fibrin clot (middle layer) was separated from the tube by discarding the bottom part containing red blood cells. The fibrin clot was gently squeezed with a non-absorbable sterile material in a petri dish to extrude HAS. HAS was pooled from the individual 15 blood donors and stored at −80 °C. Leukocyte poor PRP (lpPRP) was prepared by transferring whole blood from the same donors onto 9 mL EDTA coated blood collection tubes (VACUETTE® K3EDTA, Greiner bio-one, Kremsmünster, Austria) and centrifuged at 440 g for 10 min. The platelet enriched plasma (middle layer) along the poor platelet plasma (top layer) was further transferred to 15 mL falcon tubes leaving the leukocytes and RBC region and secondary centrifugation at 1770 g for 10 min was performed. The resulting lpPRP was pooled from individual donors and stored at −80 °C. Pooled lpPRP enclosed on average 1 × 106 platelets/mL.
Vacuette z serum clot activator
Manufactured by Greiner
Sourced in Austria
The VACUETTE® Z Serum Clot Activator is a laboratory collection tube designed for the collection and processing of blood samples. It contains a clot activator to facilitate the clotting process, allowing for the separation of serum from the blood sample.
Automatically generated - may contain errors
Lab products found in correlation
Vacuette k3edta, by Greiner (2 mentions)
Architect c8000, by Abbott (2 mentions)
Cobas c system, by Roche (2 mentions)
Cobas e801 system, by Roche (2 mentions)
Human el assay kit, by Takara Bio (2 mentions)
Cobas 8000, by Roche (2 mentions)
Vacuette, by Greiner (2 mentions)
Quantiglo, by R&D Systems (1 mentions)
Elecsys e411, by Roche (1 mentions)
Immuchem coated tube progesterone 125 1 ria kit, by MP Biomedicals (1 mentions)
12 protocols using vacuette z serum clot activator
1
Preparation of Hyper-Activated Serum and Leukocyte-Poor Platelet-Rich Plasma
2
Serum Biomarkers in Heart Failure
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Blood samples for routine laboratory assays were obtained from the AHF patients at admission to the hospital. The blood was collected in 6 mL tubes, VACUETTE® Z Serum Clot Activator (Greiner Bio-one GmbH, Kremsmuenster, Austria) with a special coating on the internal wall of the tubes containing microscopic silica particles to prevent clot formation by surface activation. Serum creatinine, urea, total plasma cholesterol, LDL cholesterol, HDL-cholesterol and triglycerides were measured using a Beckman Coulter instrument AU 2700, 2007 (Brea, CA, SAD) and Architect c8000, Abbott 2013 (Chicago, IL, SAD). Glomerular filtration rate (GFR) was calculated as described [26 (link)]. The serum aliquots were stored and transported at -80°C.
3
Routine Laboratory Assays for Cardiovascular Biomarkers
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For routine laboratory assays blood was obtained at admission to the hospital. It was collected in 6 mL tubes, VACUETTE® Z Serum Clot Activator (Greiner Bio-one GmbH, Kremsmuenster, Austria). The serum aliquots were stored at −80 °C. The Beckman Coulter instrument AU 2700, 2007 (Brea, CA, USA) and Architect c8000, Abbott 2013 (Chicago, IL, USA) were used to measure total plasma cholesterol, LDL-cholesterol, HDL-cholesterol, triglycerides and CRP. A specific chemiluminescent ELISA (QuantiGlo; R&D Systems, Wiesbaden-Nordenstadt, Germany) was used to measure IL-6 concentrations and with Human Endothelial Lipase Assay Kit (TaKaRa, Takara Bio Europe S.A.S., Saint-Germain-en-Laye, France) were measured pre-heparin EL protein levels, according to the manufacturer’s instructions.
4
Human Serum Albumin and Platelet-Rich Plasma Isolation
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Whole blood was collected from 10 individual blood donors of 37.2 ± 9.6 years (mean ± SD) into 9-mL silicon-coated blood collection tubes (VACUETTE® z serum clot activator, Greiner bio-one) and centrifuged at 1,770 g for 10 min. The resulting fibrin clot was removed from the tube, and a bottom portion containing the red blood cells was cut and discarded. The fibrin clot was then squeezed with a non-absorbable impermeable sterile material in a sterile Petri dish to extrude the HAS, and the HAS samples were stored at −80°C until further use. PRP was prepared by transferring whole blood from the same donors into 9-mL EDTA-coated blood collection tubes (VACUETTE® K3EDTA, Greiner bio-one) and centrifuged at 440 g for 10 min. The platelet enriched plasma (middle layer) along the platelet poor plasma (supernatant) was transferred into a 15-mL falcon tube and centrifuged at 1,770 g for 10 min. The resulting pellets that contained an average of 107 platelets/mL were resuspended in the superficial plasma layer corresponding to the volume obtained from the HAS in these individual donors. The PRP was stored at −80°C until further use.
5
Fasting Serum Biomarker Measurement
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A sample of venous blood was obtained from each individual after 8 to 12 h of fasting. The blood was collected in four 9 mL tubes: VACUETTE® Z Serum Clot Activator (Greiner Bio-One GmbH, Kremsmuenster, Austria). The tubes were incubated for 30 min at room temperature and subsequently centrifuged at 1800× g for 10 min at 4 °C. Total cholesterol, HDL-C, triglycerides, and CRP were measured using the Cobas c system (Roche Diagnostics, Hitachi, Tokyo, Japan) and LDL-C was calculated using the Sampson equation [46 (link)]. The calculated LDL-C values are shown along with other routine laboratory parameters in Table 2 and were not used for correlation analyses. Other routine laboratory analyses, including serum glucose, total protein, albumin, bilirubin, ALT, AST, AP, GGT, LDH, CK, creatinine, urea, urate, sodium, potassium, and chloride, were measured using Cobas 8000 (Roche Diagnostics, Hitachi, Tokyo, Japan). eGFR was calculated according to Levey et al. [47 (link)]. EL serum levels were measured using the Human EL-Assay Kit (TaKaRa, Takara Bio Europe S.A.S., Saint-Germain-en-Laye, France), as described previously [7 (link)]. IL-6 was quantified by electro-chemiluminescence immunoassay using the Cobas e801 system (Roche Diagnostics, Hitachi, Tokyo, Japan).
6
Comprehensive Metabolic Profiling of Fasted Individuals
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A sample of venous blood was obtained from each individual after 8 to 12 h of fasting and within 15 min before the assessment of vascular function. The blood was collected in four 9 mL tubes of a VACUETTE® Z Serum Clot Activator (Greiner Bio-One GmbH, Kremsmuenster, Austria). The tubes were incubated for 30 min at room temperature and subsequently centrifuged at 1800× g for 10 min at 4 °C. Total cholesterol, HDL-C, triglycerides, and CRP were measured by using the Cobas c system (Roche Diagnostics, Hitachi, Tokyo, Japan) and LDL-C was calculated using Friedewald’s formula [58 (link)]. Other routine laboratory analyses, including serum glucose, total protein, albumin, bilirubin, ALT, AST, AP, GGT, LDH, CK, creatinine, urea, urate, sodium, potassium, and chloride, were measured using Cobas 8000 (Roche Diagnostics, Hitachi, Tokyo, Japan). eGFR was calculated according to Levey et al. [59 (link)]. EL serum levels were measured using a Human EL-Assay Kit (TaKaRa, Takara Bio Europe S.A.S., Saint-Germain-en-Laye, France), as described previously [17 (link)]. IL-6 was quantified by electro-chemiluminescence immunoassay using the Cobas e801 system (Roche Diagnostics, Hitachi, Tokyo, Japan).
7
Serum Collection and Storage Protocol
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The blood was collected from the jugular vein by vacuum tube system (Vacuette®, Greiner Bio-one International, Kremsmünster, Austria) using 10 mL serum vacutainer tubes with coagulant (Vacuette® Z Serum Clot Activator, Greiner Bio-one International, Kremsmünster, Austria). The blood samples were kept at room temperature for 2 h to allow for clotting. The serum was separated by centrifugation at 3000× g for 15 min. Samples were stored at −80 °C until analysis, which was performed within a maximum of 8 weeks.
8
Biomarker Analysis of Blood Samples
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Blood samples were obtained at admission to the hospital. The blood was collected in 6 mL tubes, VACUETTE® Z Serum Clot Activator (Greiner Bio-one GmbH, Kremsmuenster, Austria). Beckman Coulter instrument AU 2700, 2007 (Brea, CA, SAD) and Architect c8000, Abbott 2013 (Chicago, IL, SAD) were used for analysis of serum albumin, creatinine, urea, CRP, total plasma cholesterol, LDL-cholesterol, HDL-cholesterol, and triglycerides. GFR was calculated as previously described35 (link). Electrochemiluminescence immunoassay with Elecsys e411 (Roche Diagnostics GmbH, Mannheim, Germany) was used for NT-proBNP quantification.
9
Blood Sample Collection for Allergy Testing
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Blood specimens were collected at the Children Pulmonology and Allergology Centre of Vilnius University Children Hospital. The study was approved by the Lithuanian Bioethics Committee (approval No. 6B-12-306).
From each participant 4–5 ml of blood were collected by venepuncture. Blood samples were collected into 6 ml non-heparinised tubes (Vacuette Z Serum Clot Activator, Greiner Bio-one, Austria). After coagulation, blood serum was collected into 3.5 ml tubes (Röhner Tubes, Sarstedt, Germany). Immediately after collection, the aliquots of serum were used to perform allergy testing and the remaining samples were frozen and stored at –70°C for further analysis.
From each participant 4–5 ml of blood were collected by venepuncture. Blood samples were collected into 6 ml non-heparinised tubes (Vacuette Z Serum Clot Activator, Greiner Bio-one, Austria). After coagulation, blood serum was collected into 3.5 ml tubes (Röhner Tubes, Sarstedt, Germany). Immediately after collection, the aliquots of serum were used to perform allergy testing and the remaining samples were frozen and stored at –70°C for further analysis.
10
Bovine blood collection protocol
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The blood was collected from the jugular vein using a permanent intravenous catheter (Intraflow 2 (PTFE) 80 mm × 2.7 mm, Vygon, Ecouen, France) or a vacuum tube system (Vacuette®, Greiner Bio-one International, Kremsmünster, Austria) using 10 mL serum vacutainer tubes with coagulant (Vacuette® Z Serum Clot Activator, Greiner Bio-one International, Kremsmünster, Austria). The permanent intravenous catheter was inserted 24 h prior to the first sample withdrawal. When the catheter became non-functional, the vacuum tube system was used (21 out of 208 samples). Here, the cows were restrained using head gates and a chain was used to hold off the jugular vein. All blood samples were kept at room temperature for 2 h to allow clotting. The serum was separated by centrifugation at 3000× g for 15 min. Samples were stored at −80 °C until analysis which was performed within a maximum of 8 weeks.
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