The standard compounds and reagents (all of analytical grade): MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DMSO (dimethyl sulfoxide), gallic acid, Na2CO3, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), Folin-Ciocalteu’s reagent, resazurin, tetracycline, chloramphenicol, kanamycin, acetonitrile (LC-MS grade, gradient grade), formic acid, 4-methoxybenzoic acid, 4-hydroxy-3-methoxycinnamaldehyde, caffeic acid, quercetin, luteolin and pinostrobin PBS were purchased from Merck (Darmstadt, Germany). The standards of 4-hydroxybenzoic acid, p-coumaric acid, ferulic, isoferulic acid, pinobanksin, chrysin, sakuranetin, naringenin, apigenin, kaempferol, isorhamnetin, acacetin and pinocembrin were obtained from Extrasynthese (Genay, France) and galangin from Alfa Aesar, (Haverhill, MA, USA). The antibiotics: ampicillin, oxacillin, teicoplanin, gentamicin, amikacin, fusidic acid, erythromycin, mupirocin, rifampicin, levofloxacin, norfloxacin, linezolid, bacitracin were purchased from Argenda (Poland). Methanol, ethanol, vanillin, benzoic acid, cinnamic acid were purchased from (POCH, Gliwice, Poland). Ultrapure H2O (18.0 MΩ) was obtained with a Milli-Q Advantage A10 system (Millipore, Billerica, MA, USA). The absorbance of the reaction mixture in Folin-Ciocalteu, DPPH assays were measured using a Genesys 20 spectrophotometer (Thermo Scientific, Waltham, MA, USA).
Ferulic
Manufactured by Extrasynthese
Sourced in France
Ferulic is a laboratory equipment product designed for use in scientific research and analysis. It serves as a key component in various experimental setups and procedures. The core function of Ferulic is to facilitate the analysis and measurement of chemical compounds or biological samples, though its specific applications may vary depending on the intended use by the research team.
Automatically generated - may contain errors
Lab products found in correlation
Kaempferol, by Extrasynthese (2 mentions)
Formic acid, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Luteolin, by Merck Group (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Chloramphenicol, by Merck Group (1 mentions)
Tetracycline, by Merck Group (1 mentions)
Resazurin, by Merck Group (1 mentions)
Folin ciocalteu s reagent, by Merck Group (1 mentions)
P coumaric acid, by Extrasynthese (1 mentions)
4 methoxybenzoic acid, by Merck Group (1 mentions)
Apigenin, by Extrasynthese (1 mentions)
Naringenin, by Extrasynthese (1 mentions)
Isoferulic acid, by Extrasynthese (1 mentions)
Sakuranetin, by Extrasynthese (1 mentions)
Isorhamnetin, by Extrasynthese (1 mentions)
Pinocembrin, by Extrasynthese (1 mentions)
Galangin, by Thermo Fisher Scientific (1 mentions)
3 protocols using ferulic
1
Standardized Phytochemical Characterization
2
Analytical Standards Preparation and Recombinant Enzyme Purification
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Ethanol, hexane and formic acid of analytical reagent grade were from Sigma-Aldrich (Steinheim, Germany). Acetonitrile of HPLC and HPLC-MS grade were purchased from Panreac (Barcelona, Spain). The Milli-Q-system (Millipore SA, Molsheim, France) was used to produce deionized water. Pinoresinol (≥ 95%) and pcoumaric acid (≥ 98%) from Sigma-Aldrich (Steinheim, Germany) were used as standard compounds. Other standards as vanillic, ferulic and syringic acids, Eresveratrol, tyrosol, and kaempferol were purchased from Extrasynthese (France).
Human recombinant PTP-1B was expressed in Escherichia coli TB1 strain, and purified as previously described (Paoli et al., 2013) . 4-nitrophenyl phosphate disodium salt hexahydrate were from Santa Cruz Biotechnology.
Human recombinant PTP-1B was expressed in Escherichia coli TB1 strain, and purified as previously described (Paoli et al., 2013) . 4-nitrophenyl phosphate disodium salt hexahydrate were from Santa Cruz Biotechnology.
3
HPLC Analysis of Phenolic Compounds
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HPLC-grade acetonitrile was purchased from Fisher Scientific (Lisbon, Portugal). Formic acid was purchased from Prolabo (VWR International, France). The phenolic compound standards (ferulic, sinapic, p-coumaric and caffeic acids, and kaempferol-3-O-rutinoside, quercetin-3-O-rutinoside, and quercetin-3-O-glucoside) were purchased from Extrasynthese (Genay, France). All other chemicals were of analytical grade and were purchased from common sources. Water was treated in a Milli-Q water purification system (Millipore, model A10, Billerica, MA, USA).
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