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Methoxy polyethylene glycol amine mpeg nh2

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Methoxy polyethylene glycol amine (mPEG-NH2) is a functionalized polyethylene glycol (PEG) polymer with a primary amine group. It is commonly used as a reagent in various bioconjugation and modification applications.

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Lab products found in correlation

Polyacrylic acid, by Merck Group (4 mentions) 4 morpholineethanesulfonic acid, by Merck Group (4 mentions) 2 amino 2 hydroxymethyl 1 3 propanediol, by Merck Group (4 mentions) Poly maleic anhydride alt 1 octadecene, by Merck Group (3 mentions) Oleic acid, by Merck Group (3 mentions) 1 octadecene, by Merck Group (3 mentions) Phosphate buffered saline (pbs), by Merck Group (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Chloroform, by Merck Group (2 mentions) Ez link sulfo nhs lc biotin, by Thermo Fisher Scientific (2 mentions) Draq5 fluorescent probe, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by Merck Group (2 mentions) Anti cd8α monoclonal antibody clone 2.43, by BioXCell (2 mentions) 4 dimethylaminopyridine, by Merck Group (2 mentions) Hexane, by Thermo Fisher Scientific (2 mentions) Chloroform, by Thermo Fisher Scientific (2 mentions) Toluene, by Thermo Fisher Scientific (2 mentions) Oleylamine, by Merck Group (2 mentions) Di water, by Thermo Fisher Scientific (2 mentions)

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MPEG–NH2 (2 citations)

6 protocols using methoxy polyethylene glycol amine mpeg nh2

1

Rare-earth Nanoparticle Synthesis and Functionalization

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Rare-earth(III) acetate hydrate (RE: Yb, Er, Ce), zinc acetate, sodium trifluoroacetate, oleic acid (OA), 1-octadecene (ODE), sodium hydroxide, ammonium fluoride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), Poly(acrylic acid) (PAA; average Mw 1800), poly(maleic anhydride-alt-1-octadecene) (PMH; 30–50 kDa), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), cyclohexane, chloroform, 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base), fetal bovine serum (FBS), phosphate buffered saline (PBS), and RPMI-1640 medium were purchased from Sigma-Aldrich and used without further purification. Yttrium(III) oxides and trifluoroacetic acid (99%) were purchased from Alfa Asea and used as received. Methoxy polyethylene glycol amine (mPEG-NH2; 5000 m.W.) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH2; 40 kDa) was purchased from Advanced Biochemicals. Anti-PD-L1 monoclonal antibody (Atezolizumab, Clone SP142) was purchased from Selleckchem. Anti-CD8α monoclonal antibody (Clone 2.43) was purchased from Bio X Cell. EZ-Link™ Sulfo-NHS-LC-Biotin and DRAQ5 Fluorescent Probe were purchased from Thermo Fisher Scientific. Streptavidin was purchased from ProSpec. Y(CF3COO)3 was prepared by the literature method.51 Detailed information on the antibodies is included in the Life Sciences Reporting Summary.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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2

Synthesis and Characterization of mPEG-Ce6 Conjugates

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Methoxy polyethylene glycol amine [mPEG-NH2, molecular weight (Mw) = 5 kDa] was purchased from Laysan Bio Inc. (Alabama, USA). TCPO and sodium cyanoborohydride were purchased from Tokyo Chemical Industry (Tokyo, Japan). CMD sodium salt (Mw = 10 to 20 kDa), N-(3-dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC•HCl), 4-(dimethyl amino)pyridine (DMAP), dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), 2′,7′-dichlorofluorescin diacetate (DCF-DA), TMP, DMPO, and [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The Ce6 was obtained from Frontier Scientific Inc. (Logan, Utah, USA). Human colorectal adenocarcinoma cells (HT29) were purchased from the American Type Culture Collection (Rockville, MD, USA). For cell culture, RPMI 1640 media, antibiotic-antimycotic solution, trypsin-EDTA, and Dulbecco’s PBS (DPBS) were purchased from Welgene Inc. (Daegu, Korea). All experiments involving live animals were carried out in accordance with the relevant laws and institutional guidelines of Sungkyunkwan University.
Jeon J., You D.G., Um W., Lee J., Kim C.H., Shin S., Kwon S, & Park J.H. (2020). Chemiluminescence resonance energy transfer–based nanoparticles for quantum yield–enhanced cancer phototheranostics. Science Advances, 6(21), eaaz8400.
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3

Synthesis of Lanthanide-Doped Nanoparticles

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YCl3·6H2O (99.99%), YbCl3·6H2O (99.99%), ErCl3·6H2O (99.99%), CeCl3·6H2O (99.99%), NdCl3·6H2O (99.99%), NH4F (99.99%), NaOH (99.9%), oleic acid (OA, 90%), 1-octadecene (ODE, 90%), poly(maleic anhydride-alt-1-octadecene) (PMH; 3000-5000 m. W.), 4-(dimethylamino)pyridine (DMAP), 4-morpholineethanesulfonic acid (MES), tris hydrochloride (Tris-HCl), 1-(3-dimethylaminopropyl)−3-ethylcarbodiimide hydrochloride (EDC) were purchased from Sigma-Aldrich. Cyclohexane, chloroform, ICG were bought from Aladdin Co. Ethanol (99.7%) and methanol (99.7%) were purchased from Shenzhen hs-science Co. Methoxy polyethylene glycol amine (mPEG-NH2; 5000 m.W.) was purchased from Laysan-Bio and Cy3-mPEG-NH2 (2000 m.W.) was purchased from Xi’an ruixibio Co. All reagents were used as received without further purification.
Mi C., Zhang X., Yang C., Wu J., Chen X., Ma C., Wu S., Yang Z., Qiao P., Liu Y., Wu W., Guo Z., Liao J., Zhou J., Guan M., Liang C., Liu C, & Jin D. (2023). Bone disease imaging through the near-infrared-II window. Nature Communications, 14, 6287.
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4

Biofunctionalized Nanoparticle Synthesis

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Lead (II) chloride (PbCl2), 1-octadecene (ODE), cadmium oxide (CdO) and sulfur powder (sublimed) were purchased from Alfa Aesar. Oleylamine, oleic acid, 1-octadecene, poly(maleic anhydride-alt-1-octadecene) (PMH; average molecular weight: 30k-50k), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), poly(acrylic acid) (PAA; average molecular weight: 1800), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base) were purchased from Sigma-Aldrich. Hexane, toluene, chloroform, and DI water were purchased from Fisher Scientific. Methoxy polyethylene glycol amine (mPEG-NH2; average molecular weight: 5 kD) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH2‧HCl; average molecular weight: 40 kDa) was purchased from Advanced Biochemicals. All the chemicals were used without further purification. 50 μm capillaries were bought from VitroCom. Purified anti-mouse/human PNAd antibody (Clone: MECA-79), purified anti-mouse CD169 antibody (Clone: 3D6.112) and DRAQ7 (# 424001) were purchased from BioLegend. Anti-mouse CD3 antibody (Clone: KT3) was bought from Bio X Cell.
Wang F., Ren F., Ma Z., Qu L., Gourgues R., Xu C., Baghdasaryan A., Li J., Zadeh I.E., Los J.W., Fognini A., Qin-Dregely J, & Dai H. (2022). In vivo Non-Invasive Confocal Fluorescence Imaging Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors. Nature nanotechnology, 17(6), 653-660.
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5

Biofunctionalized Nanoparticle Synthesis

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Lead (II) chloride (PbCl2), 1-octadecene (ODE), cadmium oxide (CdO) and sulfur powder (sublimed) were purchased from Alfa Aesar. Oleylamine, oleic acid, 1-octadecene, poly(maleic anhydride-alt-1-octadecene) (PMH; average molecular weight: 30k-50k), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), poly(acrylic acid) (PAA; average molecular weight: 1800), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base) were purchased from Sigma-Aldrich. Hexane, toluene, chloroform, and DI water were purchased from Fisher Scientific. Methoxy polyethylene glycol amine (mPEG-NH2; average molecular weight: 5 kD) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH2‧HCl; average molecular weight: 40 kDa) was purchased from Advanced Biochemicals. All the chemicals were used without further purification. 50 μm capillaries were bought from VitroCom. Purified anti-mouse/human PNAd antibody (Clone: MECA-79), purified anti-mouse CD169 antibody (Clone: 3D6.112) and DRAQ7 (# 424001) were purchased from BioLegend. Anti-mouse CD3 antibody (Clone: KT3) was bought from Bio X Cell.
Wang F., Ren F., Ma Z., Qu L., Gourgues R., Xu C., Baghdasaryan A., Li J., Zadeh I.E., Los J.W., Fognini A., Qin-Dregely J, & Dai H. (2022). In vivo Non-Invasive Confocal Fluorescence Imaging Beyond 1700 nm Using Superconducting Nanowire Single-Photon Detectors. Nature nanotechnology, 17(6), 653-660.
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6

Rare-earth Nanoparticle Synthesis and Functionalization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rare-earth(III) acetate hydrate (RE: Yb, Er, Ce), zinc acetate, sodium trifluoroacetate, oleic acid (OA), 1-octadecene (ODE), sodium hydroxide, ammonium fluoride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), Poly(acrylic acid) (PAA; average Mw 1800), poly(maleic anhydride-alt-1-octadecene) (PMH; 30–50 kDa), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), cyclohexane, chloroform, 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base), fetal bovine serum (FBS), phosphate buffered saline (PBS), and RPMI-1640 medium were purchased from Sigma-Aldrich and used without further purification. Yttrium(III) oxides and trifluoroacetic acid (99%) were purchased from Alfa Asea and used as received. Methoxy polyethylene glycol amine (mPEG-NH2; 5000 m.W.) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH2; 40 kDa) was purchased from Advanced Biochemicals. Anti-PD-L1 monoclonal antibody (Atezolizumab, Clone SP142) was purchased from Selleckchem. Anti-CD8α monoclonal antibody (Clone 2.43) was purchased from Bio X Cell. EZ-Link™ Sulfo-NHS-LC-Biotin and DRAQ5 Fluorescent Probe were purchased from Thermo Fisher Scientific. Streptavidin was purchased from ProSpec. Y(CF3COO)3 was prepared by the literature method.51 Detailed information on the antibodies is included in the Life Sciences Reporting Summary.
Zhong Y., Ma Z., Wang F., Wang X., Yang Y., Liu Y., Zhao X., Li J., Du H., Zhang M., Cui Q., Zhu S., Sun Q., Wan H., Tian Y., Liu Q., Wang W., Garcia K.C, & Dai H. (2019). In vivo molecular imaging for immunotherapy using ultra-bright near-infrared-IIb rare-earth nanoparticles. Nature biotechnology, 37(11), 1322-1331.
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