Log In Sign up
The largest database of trusted experimental protocols

Igg1 fc

Manufactured by Sino Biological
Visit Supplier
Sourced in China

IgG1-Fc is a recombinant protein that consists of the Fc region of the human IgG1 antibody. It is a useful tool for various research applications, such as the study of Fc receptor binding and antibody-dependent cellular cytotoxicity (ADCC).

Automatically generated - may contain errors

Lab products found in correlation

Cd80 fc, by BioLegend (2 mentions) Anti egfp mab, by Abcam (1 mentions) Human recombinant pd l1 fc, by BioLegend (1 mentions) Soluble anti cd28 37.51, by BD (1 mentions) Magnisort mouse cd8 t cell enrichment kit, by Thermo Fisher Scientific (1 mentions) Anti cd3ε 145 2c11, by BD (1 mentions) Pd l1 fc, by BioLegend (1 mentions)

Spelling variants of this product

HRP conjugated anti-human IgG1 Fc antibody (2 citations)

5 protocols using igg1 fc

1

Protein-Protein Interaction Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified recombinant human FAM3B-His protein was incubated with commercially available purified recombinant human IgG1 Fc or FGFR-Ecto-Fc protein (the ectodomain of human FGFRs fused to the Fc region of human IgG1 at the C terminus) from Sino Biological (IgG1 Fc, catalog no. 10702-HNAH; FGFR1-Ecto-Fc, catalog no. 16482-H02H; FGFR2-Ecto-Fc, catalog no. 10824-H03H; FGFR3-Ecto-Fc, catalog no. 16044-H02H; FGFR4-Ecto-Fc, catalog no. 10538-H02H) in binding buffer containing 1× PBS and 0.1% Nonidet P-40 with top-to-bottom rotation at 4 °C for 4 h. Except for a small fraction of input, the mixture was further incubated with Protein A superparamagnetic beads (Invitrogen, catalog no. 10-001-D) with rotation at 4 °C for 1 h. Then the beads were washed with binding buffer four times (10 min per wash without changing tubes) and finally denatured in 2× loading buffer at 100 °C for 5 min followed by SDS-PAGE and Western blot.
Zhang F., Zhu X., Wang P., He Q., Huang H., Zheng T., Li Y., Jia H., Xu L., Zhao H., Colozza G., Tao Q., De Robertis E.M, & Ding Y. (2021). The cytokine FAM3B/PANDER is an FGFR ligand that promotes posterior development in Xenopus. Proceedings of the National Academy of Sciences of the United States of America, 118(20), e2100342118.
+ Open protocol
+ Expand
2

Virus Particle Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus particles were incubated overnight at 4°C with 2 μg of Fc-chimera protein TIM-1-Fc (AdipoGen) or IgG1-Fc (Sino Biological) in TBS containing 10 mM CaCl2. BSA-saturated protein G Sepharose beads (GE Healthcare) were added and incubated for 4 h at 4°C. Beads were washed with TBS containing 10 mM CaCl2 and 0.05% Tween, and bound material was resolved in Laemmli buffer under nonreducing conditions, followed by loading onto 10% SDS-PAGE gels and electroblotting onto PVDF membranes. PVDF-bound virus particles were detected with the anti-flavivirus group-specific MAb 4G2, anti-EGFP MAb (Abcam) recognizing fused VP40 of EBOV-VLPs, or anti-matrix protein 1 (M1) of influenza A virus H1N1 (PR8) MAb (Sino Biological), followed by incubation with HRP-conjugated rabbit anti-mouse IgG antibodies (Sigma-Aldrich).
Zhang X., Liang C., Wang H., Guo Z., Rong H., Pan J., Li W., Pei R., Chen X., Zhang Z., Zhang X.E, & Cui Z. (2022). T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio, 13(1), e02860-21.
+ Open protocol
+ Expand
3

Recombinant Human Fc Fragment Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
The recombinant human IgG2 Fc, IgG1 Fc and IgG1 Fc-YTE fragments were purchased from Sino Biological Inc. (Beijing China) using their proprietary expression system and purification protocols. Briefly, the DNA encoding the mature Fc fragments were cloned into a CMV promoter driven expression vector and the proteins were expressed in HEK293 cells by transient transfection, then purified on a protein A column (Sino). The Fc proteins were dialyzed into a final buffer of 20 mM Tris pH 7.5, 50 mM NaCl
Remesh S.G., Armstrong A.A., Mahan A.D., Luo J, & Hammel M. (2018). Conformational Plasticity of the Immunoglobulin Fc Domain in Solution. Structure (London, England : 1993), 26(7), 1007-1014.e2.
+ Open protocol
+ Expand
4

Activation of Human T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flat-bottom 96-well plates were coated with the described amounts of anti-CD3 (OTK3; eBioscience) and human recombinant PD-L1-Fc (Biolegend), CD80-Fc (Biolegend), or IgG1-Fc (Sino Biological) diluted in PBS overnight at 4 °C. Mixture was aspirated, and PBMCs were cultured with soluble anti-CD28 (CD28.2; BD Biosciences) for the described amounts of time.
Marshall N., Hutchinson K., Marron T.U., Aleynick M., Hammerich L., Upadhyay R., Svensson-Arvelund J., Brown B.D., Merad M, & Brody J.D. (2019). Anti-tumor T-cell homeostatic activation is uncoupled from homeostatic inhibition by checkpoint blockade. Cancer discovery, 9(11), 1520-1537.
+ Open protocol
+ Expand
5

Stimulation of CD8 T-cells with PD-L1 and CD80

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions of splenocytes were CD8 T-cell-enriched with MagniSort mouse CD8 T-cell enrichment kit (Invitrogen). Flat-bottom 96-well plates were coated with the described amounts of anti-CD3ε (145–2C11; BD Biosciences) and mouse recombinant PD-L1-Fc (Biolegend), CD80-Fc (Biolegend), or IgG1-Fc (Sino Biological) diluted in PBS overnight at 4 °C. The mixture was aspirated, and CD8 T-cells were cultured with soluble anti-CD28 (37.51; BD Biosciences) for the described amounts of time.
Marshall N., Hutchinson K., Marron T.U., Aleynick M., Hammerich L., Upadhyay R., Svensson-Arvelund J., Brown B.D., Merad M, & Brody J.D. (2019). Anti-tumor T-cell homeostatic activation is uncoupled from homeostatic inhibition by checkpoint blockade. Cancer discovery, 9(11), 1520-1537.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!