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4 protocols using milli q direct 8 millipore system

1

Chiral HPLC Analysis of Enantiomers

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R- and S-APR were purchased from Beijing Mesochem Technology Co. Ltd. (Beijing, China). Human serum albumin was ordered from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) of gradient grade were purchased from Merck (Darmstadt, Germany). The immobilized Chiral-HSA column (4.0 × 100 mm, 5 μm) was ordered from Chiral Technologies Inc., a subsidiary of Daicel Chemical Industries Ltd. (Tokyo, Japan). Analytical-grade acetic acid, phosphate salts, phosphoric acid, sodium hydroxide and ammonium–acetate were ordered from Sigma-Aldrich, Hungary (Budapest, Hungary). These were used for the preparation of the buffer solution. All reagents were used without further purification. The deionized water was prepared by a Milli-Q Direct 8 Millipore system (Millipore Corporation, Bedford, MA, USA).
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2

Synthesis of Selenocystine Compounds

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Seleno-l-cystine, oxidized glutathione, sarcosine and tert-butylamine were obtained from Sigma Aldrich, dithiothreitol was obtained from Tokyo Chemical Industry, selenocystamine dihydrochloride was purchased from AKos GmbH. Deuterium oxide (D2O) and methanol were purchased from Merck. All reagents were of analytical grade and used without further purification. The deionized water was prepared with a Milli-Q Direct 8 Millipore system.
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3

Cyclodextrin-Based Host-Guest Interactions

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POM was obtained from Beijing Mesochem Technology Co. Ltd. (Beijing, China). D2O, DMSO-d6 and DMSO were ordered from Sigma-Aldrich Hungary (Budapest, Hungary). All native CDs (α-, β- and γ-CD) and their derivatives (hydroxypropyl-β-CD (HP-β-CD) with different degrees of substitution (DS) 3, 4.5, 6; randomly methylated-β-CD DS~12 (RAMEB), permethylated-β-CD (TRIMEB), sulfobutylether-β-CD DS~7 (SBE-β-CD)) were the products of Cyclolab R&D Ltd. (Budapest, Hungary). All other chemicals used were of analytical grade from commercial suppliers. Ultrapure, deionized water was prepared by a Milli-Q Direct 8 Millipore system (Milford, MA, USA).
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4

Chiral LC-MS Analysis of Proton Pump Inhibitors

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Macrocyclic glycopeptides-type chiral columns with identical dimensions (100 × 4.6 mm, 5-μm particle size)ristocetin A-based Chirobiotic R, teicoplanin-based Chirobiotic T, teicoplanin aglycone-based Chirobiotic TAG, and vancomycin-based Chirobiotic V were purchased from Supelco/Astec, (Milwaukee, Wisconsin). Pantoprazole sodium hydrate (≥98%), esomeprazole magnesium hydrate (≥98%), and triethylamine (≥99%) were ordered from Sigma-Aldrich Hungary (Budapest). Gradient grade methanol (MeOH), acetonitrile (ACN), ethanol (EtOH), glacial acetic acid, ammonium acetate (≥98%), ammonium formate (≥99%), and formic acid (≥98%) were purchased from Merck (Darmstadt, Germany). Controloc 20-mg tablets were obtained from Central Pharmacy of Semmelweis University (Budapest, Hungary). Ultrapure, deionized water was prepared by a Milli-Q Direct 8 Millipore system (Milford, Massachusetts).
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