Transmission electron microscopy (TEM) images were recorded with a Cs-corrected STEM (JEM-ARM200F). The EuTc-doped AgNP@SiO2 samples were cast onto the copper grids (300 mesh) with a lacey carbon film (LC300-CU, Electron Microscopy Sciences) and dried at room temperature overnight. The average shell thickness and the distribution of the SiO2 shell thickness were analyzed based on the TEM images. The nanoparticle size distribution was analyzed based on at least 150 nanoparticles by using ImageJ software. Energy dispersive X-ray spectroscopy (EDS) images and element mapping data were also obtained during the TEM measurements. The UV-vis absorption spectra and fluorescence spectra were obtained with an Infinite M200 PRO Multi-Detection Microplate Reader (Tecan).
Infinite m200 pro multi detection microplate reader
Manufactured by Tecan
Sourced in Switzerland
The Infinite M200 PRO Multi-Detection Microplate Reader is a versatile laboratory instrument designed to perform various assays and measurements on microplates. It can detect and quantify a wide range of analytes, including absorbance, fluorescence, and luminescence. The device is capable of handling different microplate formats and offers multiple detection modes to meet the diverse needs of scientific research and analytical applications.
Automatically generated - may contain errors
5 protocols using infinite m200 pro multi detection microplate reader
1
Characterization of EuTc-doped AgNP@SiO2 Nanoparticles
2
Cell Viability Assay with H2O2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was measured using Cell Counting Kit‐8 (CCK‐8) (Cat. No. 96992; Sigma). Cells were first seeded overnight at equal density, 15 000 cells per well in a 96‐well plate. They were then treated with vehicle or SHIP2 inhibitor for 24 h followed by 1 mm H2O2 for 4 h and treated with WST‐8 for 1 h at 37 °C. The absorbance at 450 nm (A450 nm) was then measured using Tecan Infinite M200 PRO Multi‐Detection Microplate Reader.
3
Microneedle Transdermal Dye Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Based on the dye specific light absorption, the optical absorption–concentration curves of insulin‐FITC (Mw: 6122.88, 200 µL, 5 mg mL−1, Aladdin Reagents Co., Ltd, Shanghai) and methylene blue (Mw: 319.8, glucose substitute, 200 µL, 1 mg mL−1, Aladdin Reagents Co., Ltd, Shanghai) were established, respectively. The insulin‐FITC and methylene blue centration were quantified by the optical absorption density (O.D.) at 495 and 664 nm, respectively. Absorption–concentration analyses were performed via an Infinite M200 Pro Multi Detection Microplate Reader (Tecan, Switzerland). The tips of MMN with different porosities of 30%, 40%, 50%, and 60% penetrated a parafilm (≈127 um thickness; mimicked the stratum corneum) covered on a chamber containing PBS solution. A drop of PBS solution containing methylene blue or insulin‐FITC was dropped on the MMN bottom substrate. After a 1 h free diffusion, the optical absorption value of the dye in chamber was measured, and was converted to dye concentration via the standard concentration curve. The release amounts of dye were normalized by comparing to the total amounts in the drops applied onto MMN.
4
Antioxidant Enzyme Assays in Plant Leaves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For antioxidant enzyme assays, leaf tissues (0.3 g) were ground with a 2 mL ice-cold buffer containing 50 mM PBS (pH 7.8), 0.2 mM EDTA, 2 mM AsA, and 2% (w/v) PVP. Homogenates were centrifuged at 12,000 ×g for 20 min, and the resulting supernatants were used to determine the enzyme activity. Peroxidase (POD) activity was measured as an increase in A470 by using guaiacol as a substrate (MacAdam et al., 1992 (link)). APX activity was measured as a decrease in A290 as described by Nakano and Asada (1981) . Catalase (CAT) activity was measured as a decline in A240 in accordance with the method described by Patra et al. (1978) . Total antioxidant capacity (T-AOC) was detected by measuring the ability to reduce Fe3+ to Fe2+ by using a total antioxidant capacity assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) in accordance with the manufacturer’s instructions. All spectrophotometric analyses were conducted on an Infinite M200 PRO Multi-Detection Microplate Reader (Tecan, Männedorf, Zürich, Switzerland).
5
Rapid Biosensor Salicylic Acid Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Salicylic acid measurements were conducted using a rapid biosensor-based method as described by Defraia et al. (2008) (link). Leaf tissues were ground in liquid nitrogen and then left at room temperature for 5 min. Acetate buffer (0.1 M, pH 5.6) was added at a ratio of 2.5 μL/mg tissue at room temperature before samples were mixed and centrifuged for 15 min at 16,000 ×g. Half (100 μL) of the supernatant was stored on ice for free SA measurement, and the other half was incubated at 37°C for 90 min with 4 U of β-glucosidase (3.2.1.21, Sigma-Aldrich, St. Louis, MO, USA) for conjugated SA measurement.
An overnight biosensor culture of Acinetobacter sp. ADPWH_lux was diluted in 37°C LB (1:20) and grown for ∼3 h at 200 rpm to an OD600 of 0.4. Up to 20 μL of crude extract that was stored at room temperature (20–22°C) was added to 60 μL of LB and 50 μL of biosensor culture in a black 96-well cell culture plate. The plate was incubated at 37°C for 1 h without shaking before luminescence was read by on an Infinite M200 Pro Multi-Detection Microplate Reader (Tecan, Männedorf, Zürich, Switzerland).
An overnight biosensor culture of Acinetobacter sp. ADPWH_lux was diluted in 37°C LB (1:20) and grown for ∼3 h at 200 rpm to an OD600 of 0.4. Up to 20 μL of crude extract that was stored at room temperature (20–22°C) was added to 60 μL of LB and 50 μL of biosensor culture in a black 96-well cell culture plate. The plate was incubated at 37°C for 1 h without shaking before luminescence was read by on an Infinite M200 Pro Multi-Detection Microplate Reader (Tecan, Männedorf, Zürich, Switzerland).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!