To minimize the effect of tissue degradation on the metabolite profiles, the samples were prepared on ice block and within a short period (5 ± 1 min). The biopsies (13 ± 3 mg) were cut to fit 30 μl disposable inserts filled with 3 μl phosphate buffered saline (PBS) in D2O containing 1.0 mM TSP for chemical shift referencing and 1.0 mM Format for shimming. The HR MAS spectra were acquired on a Bruker Avance DRX600 spectrometer equipped with a 1H/13C MAS probe with gradient (Bruker Biospin GmbH, Germany) using the following parameters; 5 kHz spin rate, 4°C probe temperature, cpmgpr1D sequence (Bruker Biospin GmbH, Germany) with 273.5 ms total echo time, a spectral width of 20 ppm (−5 to 15 ppm) and 256 scans (NS). For some patients, more than one biopsy (taken from different places in the tumor) were prepared and analyzed by HR MAS MRS.
1h 13c mas probe with gradient
Manufactured by Bruker
Sourced in Germany
The 1H/13C MAS probe with gradient is a laboratory instrument designed for nuclear magnetic resonance (NMR) spectroscopy. It is capable of performing magic-angle spinning (MAS) experiments on both hydrogen (1H) and carbon-13 (13C) nuclei, while also incorporating a gradient feature for improved resolution and sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 1h 13c mas probe with gradient
1
HR MAS MRS of Tumor Biopsies
2
Metabolic Profiling of Placental Biopsies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The placental biopsies were analyzed in random order, blinded to pregnancy outcome.
Samples were prepared on a metal plate cooled in liquid nitrogen in order to minimize the effect of tissue degradation, as described in [15, 16] . Biopsies (7.5 ± 1.4 mg) were cut to fit 30µL disposable inserts (Bruker Biospin Corp, USA) filled with 3µL D 2 O containing 25mM formate for shimming. Spin-echo spectra were acquired on a Bruker Avance DRX600 spectrometer with a 1 H/ 13 C MAS probe with gradient (Bruker Biospin GmbH, Germany) using the following parameters: 5KHz spin rate, 5°C probe temperature, cpmgpr1d pulse sequence (Bruker Biospin) with 78ms total echo time, spectral width of 20 ppm and 256 scans. Two samples were additionally analyzed by 13 C-1 H spectroscopy (HSQC, HMBC) for aid in metabolite identification.
Samples were prepared on a metal plate cooled in liquid nitrogen in order to minimize the effect of tissue degradation, as described in [15, 16] . Biopsies (7.5 ± 1.4 mg) were cut to fit 30µL disposable inserts (Bruker Biospin Corp, USA) filled with 3µL D 2 O containing 25mM formate for shimming. Spin-echo spectra were acquired on a Bruker Avance DRX600 spectrometer with a 1 H/ 13 C MAS probe with gradient (Bruker Biospin GmbH, Germany) using the following parameters: 5KHz spin rate, 5°C probe temperature, cpmgpr1d pulse sequence (Bruker Biospin) with 78ms total echo time, spectral width of 20 ppm and 256 scans. Two samples were additionally analyzed by 13 C-1 H spectroscopy (HSQC, HMBC) for aid in metabolite identification.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!