Log In Sign up
The largest database of trusted experimental protocols

Anti rab7

Manufactured by Merck Group
Visit Supplier
Sourced in United Kingdom

Anti-Rab7 is a laboratory reagent used for the detection and localization of the Rab7 protein in various cellular and tissue samples. Rab7 is a small GTPase that plays a crucial role in late endosome and lysosome biogenesis and trafficking. Anti-Rab7 can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and distribution of Rab7 in different biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti clca1, by Abcam (3 mentions) Anti α synuclein, by Merck Group (2 mentions) Anti lc3, by Cell Signaling Technology (2 mentions) Anti akt, by Cell Signaling Technology (2 mentions) Anti lamp2, by Santa Cruz Biotechnology (2 mentions) Anti mtor, by Cell Signaling Technology (2 mentions) Anti β tubulin, by Merck Group (2 mentions) Anti rab4a, by Santa Cruz Biotechnology (2 mentions) Anti rab5a, by Merck Group (2 mentions) Anti rabaptin5, by Santa Cruz Biotechnology (2 mentions) Hrp conjugated anti rabbit and mouse antibodies, by GE Healthcare (2 mentions) Anti muc2, by Santa Cruz Biotechnology (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Uea1 fitc, by Vector Laboratories (1 mentions) Anti eea1, by Cell Signaling Technology (1 mentions) Anti tsg101, by GeneTex (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Anti gm130, by BD (1 mentions) Rat anti lamp1 antibody, by Developmental Studies Hybridoma Bank (1 mentions) Anti β actin, by Abcam (1 mentions)

9 protocols using anti rab7

1

Age-Dependent Regulation of Vesicular Trafficking Proteins in Entorhinal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized with ketamine (50 g/kg)/xylazine (5 mg/kg) and brains were rapidly removed over ice. Brains of 12 (n=4) and 24 (n=5) month old mice were snap frozen and stored at −80°C before biochemical analyses. Entorhinal cortices were dissected out from fresh brains of 12 (n=4) and 24 (n=4) month old mice and stored at −80°C. Western blot immunolabeling was performed as previously described52 (link). Primary antibodies used were: anti-LAMP-2 (1:1000), anti-Rab4a (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA; Cat # SC-312), anti-Rab5a (1:1000), anti-Rab7 (1:1000; Sigma, St. Louis, MO; Cat # R8779), anti-Rabaptin5 (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA; Cat # SC-6162), anti-LC3 (1:1000), anti-Cat D (1:1000), C1/6.1 (1:1000)48 (link), anti-mTOR and anti-AKT (phosphorylated and total protein) (1:1000, Cell Signaling Technology, Inc., Danvers, MA; Cat # 2983S and Cat # 9272S respectively), anti-α-synuclein (1:1000; Sigma, St. Louis, MO; Cat # S3062), and anti-β-tubulin (1:10000; Sigma, St. Louis, MO; Cat # T8535). Secondary antibodies used were: HRP conjugated anti-rabbit and mouse antibodies (1:5000; GE Healthcare, Pittsburgh, PA). The protein bands were scanned, optical density was calculated using the Image J, and the ratio of protein intensity to β-tubulin in the same lane was calculated. Western blot analyses were repeated 3 times.
Kaur G., Pawlik M., Gandy S.E., Ehrlich M.E., Smiley J.F, & Levy E. (2016). Lysosomal dysfunction in the brain of a mouse model with intraneuronal accumulation of carboxyl terminal fragments of the amyloid precursor protein. Molecular psychiatry, 22(7), 981-989.
+ Open protocol
+ Expand
2

Histological Analysis of Murine Colonic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice distal colons were sectioned (5 µm) after fixing in 4% paraformaldehyde. For histopathology analysis, sections were stained with hematoxylin and counter stained with eosin. Histological scoring of colon sections was evaluated by a blinded pathologist following these parameters: loss of epithelium (0–3), crypt damage (0–3), depletion of goblet cells (0–3), and inflammatory cell infiltrate (0–3). For immunostaining, human biopsy and mice colon samples were sectioned and after fixing with 4% paraformaldehyde antigen retrieval was performed. The sections were probed with anti-Rab7 (Sigma, R4779, 1:400), UEA1-FITC (Vector Laboratories, FL-1061, 1:400), anti-Muc2 (Santa Cruz, sc-515032, 1:200), and anti-CLCA1 (Abcam, ab180851, 1:100) in blocking (5% goat serum) overnight. Sections were incubated with HRP or fluorophore tagged secondary antibodies for 2 hr. Nucleic acid was stained with DAPI (1 µg/ml). Sections were cured and mounted with Prolong Gold Antifade Reagent (Thermo Fisher). Sections were imaged in confocal microscope (Leica SP8).
Gaur P., Rajendran Y., Srivastava B., Markandey M., Fishbain-Yoskovitz V., Mohapatra G., Suhail A., Chaudhary S., Tyagi S., Yadav S.C., Pandey A.K., Merbl Y., Bajaj A., Ahuja V, & Srikanth C. (2024). Rab7-dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis. eLife, 12, RP89776.
+ Open protocol
+ Expand
3

Antibody Detection Techniques in Prion Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following mouse antibodies were used: anti-Rab7 (Sigma), anti-Tsg101 (GeneTex), anti-β-actin (Abcam), anti-GM130 (BD Transduction Laboratories) and anti-prion (SAF32, Cayman chemical; AH6, TSE Resource Center,). The following rabbit antibodies were used: anti-Hrs (Novus Biologicals), anti-TGN38 (AbD Serotec), anti-GFP (Abcam), anti-EEA1 (Cell Signaling), anti-Vps26 (a gift from Juan Bonifacino, Cell Biology Metabolism Program, NICHD, NIH, Bethesda, MD), anti-CI-M6PR (a gift from Linton Traub, Department of Cell Biology, University of Pittsburgh, PA) and anti-Alix (Bethyl Laboratories). Rat anti-LAMP1 antibody (Developmental Studies Hybridoma Bank) was used. PrPc and PrPsc were routinely detected using DyL488, Cy3 and DyL647-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories). Western blots were probed using horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories) and InfraRed Dye 680 and 800 secondary antibodies (Li-Cor Bioscience).
Yim Y.I., Park B.C., Yadavalli R., Zhao X., Eisenberg E, & Greene L.E. (2015). The multivesicular body is the major internal site of prion conversion. Journal of Cell Science, 128(7), 1434-1443.
+ Open protocol
+ Expand
4

Multicolor Immunocytochemistry of Intestinal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded on 18 mm glass coverslips, fixed with 4% methanol-free paraformaldehyde, and blocked in 0.1% BSA + 0.01% TritonX-100 for 1 hr. Cells were probed with anti-CLCA1 (Abcam, ab180851, 1:100), anti-Muc2 (Santa Cruz, sc-515032, 1:200), anti-Rab7 (Sigma, R4779, 1:400), and Phalloidin-594 (Invitrogen, A12381, 1:400) at 4℃ overnight. Cells were further incubated with fluorophore tagged secondary antibodies for 2 hr. Nucleic acid was stained with DAPI (1 µg/ml). Coverslips were mounted with Prolong Gold Antifade Reagent (Thermo Fisher) and visualized in confocal microscope (Leica SP8) for immunocytochemistry and in Elyra PS1 (Carl Zeiss) for structured illumination microscopy.
Gaur P., Rajendran Y., Srivastava B., Markandey M., Fishbain-Yoskovitz V., Mohapatra G., Suhail A., Chaudhary S., Tyagi S., Yadav S.C., Pandey A.K., Merbl Y., Bajaj A., Ahuja V, & Srikanth C. (2024). Rab7-dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis. eLife, 12, RP89776.
+ Open protocol
+ Expand
5

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human biopsies and mice organ tissues were homogenized using tissue homogenizer (Precellys) in RIPA buffer with added protease arrest (G Biosciences) to prepare protein lysates. Cells were lysed in RIPA buffer (with protease arrest) after PBS wash. Protein amount was measured by BCA solution (Sigma). Equal amount of protein samples was separated on SDS-PAGE gel (12%) electrophoresis and transferred to nitrocellulose membrane (Bio-Rad). Blots were blocked with 5% skim milk for 1 hr at room temperature and probed with antibodies at 4℃ overnight against the desired proteins. The primary antibodies used were anti-Rab7 (Sigma, R4779, 1:5000), anti-F (Invitrogen, 39-8600, 1:2000), anti-β-actin (Cell Signaling Technology, 4970S, 1:20,000), anti-CLCA1 (Abcam, ab180851, 1:20,000) (Key Resources Table). Specific secondary antibodies conjugated with HRP were probed for 1 hr at room temperature. Blots were detected and visualized for protein bands with Immobilon Forte western HRP substrate (Millipore) and imaged in Image Quant LAS4000. Band intensities were measured using ImageJ software.
Gaur P., Rajendran Y., Srivastava B., Markandey M., Fishbain-Yoskovitz V., Mohapatra G., Suhail A., Chaudhary S., Tyagi S., Yadav S.C., Pandey A.K., Merbl Y., Bajaj A., Ahuja V, & Srikanth C. (2024). Rab7-dependent regulation of goblet cell protein CLCA1 modulates gastrointestinal homeostasis. eLife, 12, RP89776.
+ Open protocol
+ Expand
6

Antibody-based Western Blotting Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Western blotting the following antibodies were used: anti-Rab7 (R4779, Sigma), anti-prohibitin (II-14-10, Thermofisher Scientific). Rabbit polyclonal antibodies against mouse thiolase were raised against KLH‐conjugated polypeptide KLKPAFKDGGSTTAGN corresponding to the amino acids 259–274 of mouse prethiolase and affinity-purified by SCRUM Inc., Tokyo [29 (link)]. For Western blot analyses, the antibodies were used at concentrations of 2 μg/ml.
Yarmishyn A.A., Kremenskoy M., Batagov A.O., Preuss A., Wong J.H, & Kurochkin I.V. (2016). Genome-wide analysis of mRNAs associated with mouse peroxisomes. BMC Genomics, 17(Suppl 13), 1028.
+ Open protocol
+ Expand
7

Western Blot Analysis of Extracellular Vesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Density gradient fractions were loaded on a 4–20% gradient Tris-Glycine precast polyacrylamide gel (Bio-Rad, Hercules, CA), separated by standard SDS-PAGE procedures and transferred onto PVDF membranes. (Immobilon, EMD Millipore, Billerica, MA). Primary antibodies used in this study were: anti-CD63 (1:1,000, #ab217345, Abcam, Cambridge, UK), anti-Alix (1:1,000, #ABC40, EMD Millipore), anti-TSG101 (1:1,000, #PA5-31260, ThermoFisher Scientific), anti-Rab35 (1:1,000, #9690, Cell Signaling Technology, Danvers, MA), anti-Rab5B (1:5,000, #sc-598, Santa Cruz Biotechnology, Dallas, TX), anti-Rab7 (1:5,000, #R8779, Sigma-Aldrich) and anti-EEA1 (1:1,000, #07-1820, EMD Millipore). The secondary antibodies used were HRP-conjugated anti-rabbit and anti-mouse antibodies from Jackson ImmunoResearch (West Grove, PA, US). The membranes were incubated in chemiluminescent fluid (Pierce, Rockford, IL, US) for 5 min, and chemiluminescence was visualized on Reflection Autoradiography films. Ponceau staining (Sigma-Aldrich) was used as a loading control. Protein bands were quantified through the open source software ImageJ (National Institute of Health (NIH), Bethesda, MD, US). Data are shown as the densitometric ratio between Ts2 and 2N controls, after normalization for protein concentration of each fraction.
D’Acunzo P., Hargash T., Pawlik M., Goulbourne C.N., Pérez-González R, & Levy E. (2019). Enhanced Generation of Intraluminal Vesicles in Neuronal Late Endosomes in the Brain of a Down Syndrome Mouse Model with Endosomal Dysfunction. Developmental neurobiology, 79(7), 656-663.
+ Open protocol
+ Expand
8

Immunofluorescence and ADP-ribosylation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
In this study anti-Rab7 (Sigma), anti-Rab11, and anti-Hsp90 (Abcam), anti-Actin (Santa Cruz), anti-FA (7F2) (Abcam), and anti-EEA1 (Abcam) were used as primary antibodies. Secondary antibody, and fluorescent (TRITC) labeled phalloidin were purchased from Santa Cruz, and Sigma, culture materials from Nunc, and Falcon firms, DTx, and CRM197 from Calbiochem, and ProLong Gold Antifade Mountant Invitrogen firms. For ADP-ribosylation assay [Adenosine-14C]NAD (NEN-Dupont) with a specific activity of 535 Ci/mol was used.
Edis B.O., Haciosmanoglu E., Varol B, & Bektas M. (2018). Intracellular trafficking of diphtheria toxin and its mutated form, CRM197, in the endocytic pathway. Northern Clinics of Istanbul, 5(2), 89-95.
+ Open protocol
+ Expand
9

Age-Dependent Regulation of Vesicular Trafficking Proteins in Entorhinal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were anesthetized with ketamine (50 g/kg)/xylazine (5 mg/kg) and brains were rapidly removed over ice. Brains of 12 (n=4) and 24 (n=5) month old mice were snap frozen and stored at −80°C before biochemical analyses. Entorhinal cortices were dissected out from fresh brains of 12 (n=4) and 24 (n=4) month old mice and stored at −80°C. Western blot immunolabeling was performed as previously described52 (link). Primary antibodies used were: anti-LAMP-2 (1:1000), anti-Rab4a (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA; Cat # SC-312), anti-Rab5a (1:1000), anti-Rab7 (1:1000; Sigma, St. Louis, MO; Cat # R8779), anti-Rabaptin5 (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA; Cat # SC-6162), anti-LC3 (1:1000), anti-Cat D (1:1000), C1/6.1 (1:1000)48 (link), anti-mTOR and anti-AKT (phosphorylated and total protein) (1:1000, Cell Signaling Technology, Inc., Danvers, MA; Cat # 2983S and Cat # 9272S respectively), anti-α-synuclein (1:1000; Sigma, St. Louis, MO; Cat # S3062), and anti-β-tubulin (1:10000; Sigma, St. Louis, MO; Cat # T8535). Secondary antibodies used were: HRP conjugated anti-rabbit and mouse antibodies (1:5000; GE Healthcare, Pittsburgh, PA). The protein bands were scanned, optical density was calculated using the Image J, and the ratio of protein intensity to β-tubulin in the same lane was calculated. Western blot analyses were repeated 3 times.
Kaur G., Pawlik M., Gandy S.E., Ehrlich M.E., Smiley J.F, & Levy E. (2016). Lysosomal dysfunction in the brain of a mouse model with intraneuronal accumulation of carboxyl terminal fragments of the amyloid precursor protein. Molecular psychiatry, 22(7), 981-989.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!