The disodium salts of all four 5′-NMPs, viz. 5′-AMP, 5′-GMP, 5′-UMP, and 5′-CMP (all purity ≥ 98%), were purchased from Sigma-Aldrich (Bangalore, India) and used without any further purification. Analytical grade PEG 8000, dextran (average mol. wt. of 9,000- 11,000), and deuterated water (D2O) were also purchased from Sigma-Aldrich.
5 gmp
Manufactured by Merck Group
Sourced in United States
5'-GMP is a nucleotide used in the production of RNA. It serves as a building block for the synthesis of ribonucleic acid (RNA) molecules. 5'-GMP is a guanosine monophosphate derivative, which is a fundamental component of the genetic material.
Automatically generated - may contain errors
Lab products found in correlation
Dntp mixture, by Takara Bio (2 mentions)
Pyrobest dna polymerase, by Takara Bio (2 mentions)
Kod plus mutagenesis kit, by Takara Bio (2 mentions)
β mercaptoethanol β me, by Merck Group (2 mentions)
Methyl 3h sam, by PerkinElmer (2 mentions)
Tris base, by Merck Group (2 mentions)
5 ump, by Merck Group (1 mentions)
5 cmp, by Merck Group (1 mentions)
5 amp, by Merck Group (1 mentions)
Deuterated water d2o, by Merck Group (1 mentions)
Pyrophosphate, by Merck Group (1 mentions)
Nitrocellulose membranes 0.22 μm, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Guanosine 5 monophosphate disodium salt hydrate, by Merck Group (1 mentions)
Crystallization kits, by Hampton Research (1 mentions)
Adenosine triphosphate (atp), by Sangon (1 mentions)
Cytidine triphosphate, by Sangon (1 mentions)
Ni2 nta superflow, by Qiagen (1 mentions)
4 protocols using 5 gmp
1
Nucleotide Characterization and Preparation
2
Radiolabelled SAM and DMAPP Synthesis
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[Methyl-3H] SAM (78.0 Ci/mmol) was purchased from PerkinElmer Inc. (Waltham, MA, USA); [3H] DMAPP (20.0 Ci/mmol) was obtained from BIOTREND Chemicals (USA); SAM was purchased from NEW ENGLAND Biolabs Inc..; Dimethylallyl diphosphate (DMAPP), dithiothreitol (DTT), NTPs, 5′-GMP, pyrophosphate, Tris-base, β-mercaptoethanol (β-Me), MgCl2, NaCl and KCl were purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO, USA); Isopropyl β-D-thiogalactoside (IPTG) was obtained from AMRESCO (OH, USA); Nitrocellulose membranes (0.22 μm) were purchased from Merck Millipore (Darmstadt, Germany); Primers for PCR were synthesized by BioSune (Shanghai, China); Nickel-nitrilotriacetic (Ni-NTA) Superflow was purchased from Qiagen Inc.. (Germany); KOD-plus mutagenesis kit, Pyrobest DNA polymerase and the dNTP mixture were obtained from Takara (Japan); T4 polynucleotide kinase, T4 DNA ligase, Ribonuclease inhibitor and all restriction endonucleases were obtained from Fermentas/Thermo Scientific (MA, USA); The E. coli JW3581-1 and JW4129-2, strains in which the gene of TrmL and MiaA were deleted respectively, were purchased from the E. coli genetic stock center (Yale University, New Haven, CT, USA); PrepHT 300SB-C18 was purchased from Agilent Technologies (CA, USA).
3
Synthesis of Pt Catalysts under Inert Conditions
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All reactions were performed under nitrogen atmospheres using standard Schlenk techniques. K2PtCl4 (99 %) was purchased from Precious Metals Online. Guanosine 5′‐monophosphate disodium salt hydrate ≥99 % (5′‐GMP) and all other chemicals and solvents were purchased from Sigma–Aldrich and used as received.
4
Optimizing Crystallization Conditions for Methyltransferase Enzyme
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SAM, SAH, 5′-GMP, Tris-base, β-mercaptoethanol (β-Me), KCl and the reagents used to optimize crystallization conditions were purchased from Sigma–Aldrich (St Louis, MO, USA). MgCl2, NaCl, adenosine triphosphate, cytidine triphosphate, guanosine triphosphate and uridine triphosphate were from Sangon Biotech (Shanghai, China). [Methyl-3H] SAM was obtained from PerkinElmer (Waltham, MA, USA); crystallization kits were from Hampton research (Aliso Viejo, CA, USA). Primers for polymerase chain reaction were synthesized by Invitrogen (Shanghai, China) and Biosune (Shanghai, China); the pET22b vector was from Merck–Millipore (Darmstadt, Germany). The KOD-plus mutagenesis kit, Pyrobest DNA polymerase and dNTP mixture were obtained from Takara (Shiga, Japan); T4 ligase and other restriction endonucleases were obtained from MBI Fermentas (Pittsburgh, PA, USA). Pyrophosphatase (PPiase) was purchased from Roche Applied Science (Basel, Switzerland). Ni2+-NTA Superflow was obtained from Qiagen (Dusseldorf, Germany). The Superdex 200 column and 3 mm filter papers were from GE Healthcare (Fairfield, CT, USA). The analog of SAM, sinefungin, was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
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