Chromquest 5.0 chromatography software

Prior to analysis, the samples were filtered through a 0.45 μm membrane and injected into the HPLC system. HPLC-PDA analyses were performed using a Finnigan Surveyor equipped with an autosampler, a diode array detector Finnigan Surveyor-PDA Plus (Thermo FisherScientific Inc., Waltham, MA, USA) and ChromQuest 5.0 chromatography software (Thermo FisherScientific Inc., Waltham, MA, USA). The separation conditions were as described by Efenberger-Szmechtyk et al. [36 (link)]. The calibration curves were established using standards for chlorogenic acid, quercetin-glucoside and cyanidin-glucoside to quantify polyphenols at 320, 360 and 520 nm, respectively.

The organic acids and carbohydrates profiles of the tested fruit juices were determined using high performance liquid chromatography (HPLC), according to the method described by Gutarowska and Czyżowska (2009) [18 (link)]. In addition, the polyphenolic compounds were also characterized using HPLC-DAD method with a diode array detector (Finnigan Surveyor-PDA Plus detector) and a ChromQuest 5.0 chromatography software (Thermo Fisher Scientific Inc., Waltham, MA, USA) as well as using liquid chromatography mass spectrometry (LC-MS; LTQ Velos MS, Thermo Fisher Scientific) following the method described by Antolak et al. (2015) [19 (link)].

Since the interaction between Masitinib and ASP⁺ cannot indicate whether Masitinib is also transported into the cells by a specific transporter, the transport of Masitinib by OCTs and hMATE1 was quantified using reversed-phase HPLC. The mobile phase consisted of (A) 0.1% formic acid and (B) acetonitrile and was delivered at 150 µL/min in a gradient mode. After delivery of 100% A for 1 min, a linear gradient to 50% B was applied and maintained for 10 min. Thereafter isocratic elution with 100% B was applied for 5 min, and then the column was re-equilibrated with 100% A for at least 5 min (Table 2). Masitinib, detected by UV absorption at 254 nm, has a retention time of 8.6 min. An example of the chromatograms obtained by HPLC is presented in Supplementary Materials Figure S7.
The chromatographic system consisted of an Accela 600 Pump, an automatic sampler, an UV detector set to 254 nm for detection, and a degasser (Thermo Fisher Scientific, Waltham, MA, USA). Separation was carried out on an Accucore C18 column (Thermo Fisher Scientific) equipped with a C18 guard column (Phenomenex, Aschaffenburg, Germany). The data were acquired by ChromQuest 5.0 chromatography software (Thermo Fisher Scientific).