Vascular endothelial growth factor (vegf)

Manufactured by Eve Technologies

Sourced in Canada

VEGF is a lab equipment product that serves as a key factor involved in the regulation of blood vessel formation and growth. It plays a critical role in angiogenesis, the process of new blood vessel development from existing ones. VEGF is commonly used in research and scientific experiments to study vascular biology and related processes.

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4 protocols using vascular endothelial growth factor (vegf)

Synovial Fluid Cytokine and Adipokine Profiling

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The right knee joints were opened to collect synovial fluid shortly after sacrifice using the Whatman chromatography paper method^{52 (link)}. Samples were weighed, diluted and centrifuged^{40 (link)}. Serum and synovial fluid cytokines and adipokines were quantified using a Rat 27 Multiplex Discovery Assay with Luminex®xMAP technology (Eotaxin, EGF, Fractalkine, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IL-18, IP-10/CXCL10, GRO/KC, IFN-γ, TNF-α, G-CSF, GM-CSF, MCP-1, leptin, LIX, MIP-1α, MIP-2, RANTES, VEGF; Eve Technologies, AB, Canada).

Rios J.L., Bomhof M.R., Reimer R.A., Hart D.A., Collins K.H, & Herzog W. (2019). Protective effect of prebiotic and exercise intervention on knee health in a rat model of diet-induced obesity. Scientific Reports, 9, 3893.

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Multiplex Serum Cytokine Analysis in Fasted Rats

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Following 12 h of fasting, rats were anesthetized with isoflurane and a blood sample was collected by cardiac puncture. Blood was centrifuged at 3000 rpm for 15 min at 4 °C and serum stored in aliquots at − 80 °C until analyzed. Rats were sacrificed by heart excision. Serum cytokines and adipokines were quantified using a Rat 27 Multiplex Discovery Assay with Luminex® xMAP technology (eotaxin, EGF, fractalkine, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IL-18, IP-10/CXCL10, GRO/KC, IFN-γ, TNF-α, G-CSF, GM-CSF, MCP-1, leptin, LIX, MIP-1α, MIP-2, RANTES, VEGF; Eve Technologies, Calgary, AB, Canada).

Rios J.L., Boldt K.R., Mather J.W., Seerattan R.A., Hart D.A, & Herzog W. (2018). Quantifying the Effects of Different Treadmill Training Speeds and Durations on the Health of Rat Knee Joints. Sports Medicine - Open, 4, 15.

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Plasma Cytokine Profiling in Mice

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Blood was collected from the submandibular vein of all experimental mice into K₂EDTA-coated tubes (BD Biosciences, 365974) and immediately placed on ice. The blood was centrifuged for 15 minutes at 900g and 4°C, and plasma was collected. Aliquots of plasma were diluted twofold in PBS and stored at –80 °C. Blood plasma cytokines were analysed through a commercially available multiplex panel including Eotaxin, G-CSF, G M-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, CXCL1, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-2, RANTES, TNF-α, and VEGF (Eve Technologies, Alberta, Canada, MD-31).

McCallion A., Nasirzadeh Y., Lingegowda H., Miller J.E., Khalaj K., Ahn S., Monsanto S.P., Bidarimath M., Sisnett D.J., Craig A.W., Young S.L., Lessey B.A., Koti M, & Tayade C. (2022). Estrogen mediates inflammatory role of mast cells in endometriosis pathophysiology. Frontiers in Immunology, 13, 961599.

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Multiplex Cytokine Analysis of Ascites and Plasma

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Ascites fluid and plasma samples collected 24 h following initial STING agonist dose or post carboplatin chemotherapy alone from each treatment group were subjected to multiplex cytokine analysis using the mouse Cytokine Array/Chemokine Array 31-Plex Discovery Assay (includes eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-colony-stimulating factor (GM-CSF), IFN-γ, interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10 (CXCL10), CXCL1, LIF, LIX, MCP-1 (CCL2), macrophage colony-stimulating factor (M-CSF), MIG (CXCL9), MIP-1α, MIP-1β, MIP-2, RANTES (CCL5), tumor necrosis factor-α, and VEGF) at Eve Technologies (Alberta, Canada). All samples were analyzed in biological triplicates. The standard curve regression was used to calculate the concentration of each target cytokine.

Shakfa N., Li D., Conseil G., Lightbody E.D., Wilson-Sanchez J., Hamade A., Chenard S., Jawa N.A., Laight B.J., Afriyie-Asante A., Tyryshkin K., Koebel M, & Koti M. (2023). Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer. Journal for Immunotherapy of Cancer, 11(4), e006170.

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