710 laser confocal microscope

Manufactured by Zeiss

The Zeiss 710 laser confocal microscope is a high-performance imaging system designed for advanced biological and materials research. It features a state-of-the-art optical system, versatile excitation lasers, and sensitive detectors to provide superior image quality and resolution. The core function of the 710 laser confocal microscope is to enable detailed, non-invasive observation and analysis of samples at the microscopic level.

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Lab products found in correlation

Axiovision microscope, by Zeiss (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions) Deltavision software, by Cytiva (1 mentions)

Close spelling variants of this product

LSM 710 inverted confocal laser-scanning microscope LSM 710 confocal laser scanning microscope LSM 710 confocal laser scanning microscope system LSM 710 confocal laser microscope system LSM 710 Spectral Confocal Laser Scanning Microscope Zeiss 710 Meta Confocal Laser Scanning Microscope LSM 710 META confocal laser-scanning microscope LSM 710 Carl Zeiss Confocal Laser scanning microscope LSM 710 Confocal Laser Point-Scanning Microscope CLSM 710 Spectral Confocal Laser Scanning Microscope

2 protocols using 710 laser confocal microscope

Immunostaining of Glial Scar and Microglia

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Five transverse sections at epicentre and 1 mm and 2 mm rostral and caudal to the epicentre from each rat (N = 3–6 rats/group) were immunostained against GFAP and OX42 to assess astrocytic glial scar and macrophages/microglia, respectively. The frozen slides were air-dried at room temperature, and then were washed with PBS for 10 min. The sections were blocked and then incubated with primary antibodies. The following primary antibodies were used overnight at 4°C: rabbit anti-GFAP (1∶1000, Dako) for astrocytes and mouse anti-CD11b (OX42, Serotec, 1∶50). The slides were washed in PBS three times and then incubated with fluorescent Alexa 568 or 488 goat-anti mouse or anti-rabbit secondary antibodies (Invitrogen, 1∶500) for 1 hr. The slides were coverslipped with Mowiol mounting medium containing DAPI to counterstain the nuclei. The images were taken using a Zeiss 710 laser confocal microscope or a Zeiss AxioVision microscope.

Alluin O., Delivet-Mongrain H., Gauthier M.K., Fehlings M.G., Rossignol S, & Karimi-Abdolrezaee S. (2014). Examination of the Combined Effects of Chondroitinase ABC, Growth Factors and Locomotor Training following Compressive Spinal Cord Injury on Neuroanatomical Plasticity and Kinematics. PLoS ONE, 9(10), e111072.

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Fluorescence Microscopy of Resuspended Cells

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Conventional fluorescence microscopy of cells resuspended in 100 mM Tris.HCl pH 8.0, 0.2% (w/v) NaN₃ and NaF₃ was performed as previously described (Macdonald et al., 2012a (link)). For deconvolved fluorescence images, micrographs of cells imaged with an Olympus IX70 and Coolsnap HQ camera (Photometrics, Tuscon, AZ) were processed using Delta Vision software (Applied Precision Issaquah, WA). Confocal images and FRET data were collected on live cells using a Zeiss 710 laser confocal microscope. Data were processed using Fiji software and change in GFP fluorescence upon bleaching was converted to a color-coded heat map calibrated across all measurements and images.

MacDonald C., Payne J.A., Aboian M., Smith W., Katzmann D.J, & Piper R.C. (2015). A Family of Tetraspans Organizes Cargo for Sorting into Multivesicular Bodies. Developmental cell, 33(3), 328-342.

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