Epr3724

Manufactured by Abcam

EPR3724 is a primary antibody that recognizes the DNA-binding domain of the human glucocorticoid receptor. It is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.

Automatically generated - may contain errors

Lab products found in correlation

Lysis buffer, by Beyotime (1 mentions) Irdye 800cw, by LI COR (1 mentions) Lipofectamine plus, by Thermo Fisher Scientific (1 mentions) Plko vector, by Thermo Fisher Scientific (1 mentions)

2 protocols using epr3724

Quantifying Protein Expression in Glioblastoma Cells

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U87MG human glioblastoma cells; GL261 mouse glioblastoma cells; CSC2 GSCs; 83NS GSCs; normal glial cell HA 1800; astrocytes cell BV2 and endothelial cell hCMEC/D3; and liver, lung, and kidney organs were lysed using lysis buffer (Beyotime, China). The protein concentrations were quantified by BCA Protein Assay. Lysates were separated by SDS–polyacrylamide gel electrophoresis and were transferred onto polyvinylidene difluoride (PVDF) membranes. PVDF membranes were incubated with primary antibody against PLK1 [mouse monoclonal antibody (mAb) 35-206; 1:1000; Abcam], LRP-1 [rabbit mAb (EPR3724); 1:50,000; Abcam], and secondary antibody (LI-COR IRDye 800CW). Protein bands were displayed by an ECL detection system and were analyzed using ImageJ software.

Zou Y., Sun X., Yang Q., Zheng M., Shimoni O., Ruan W., Wang Y., Zhang D., Yin J., Huang X., Tao W., Park J.B., Liang X.J., Leong K.W, & Shi B. (2022). Blood-brain barrier–penetrating single CRISPR-Cas9 nanocapsules for effective and safe glioblastoma gene therapy. Science Advances, 8(16), eabm8011.

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Silencing LRP1 in SH-SY5Y Cells

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Lentiviral shRNA constructs targeted against human LRP1 (TRCN0000053253) and a control shRNA in the pLKO vector (Thermo Scientific) were transfected into human embryonic kidney 293T cells using Lipofectamine-Plus (Invitrogen). At 24 and 48 h post-transfection, culture media from transfected dishes were pooled and pelleted by centrifugation at 100,000g for 1.5 h. The lentiviral pellet was resuspended in sterile PBS and used to infect cultured SH-SY5Y cells with polybrene (4 μg/ml). Infection was repeated at 48 and 72 h after the initial addition. Knockdown efficiency was evaluated using anti-LRP1 immunoblotting (catalog no.: EPR3724; Abcam).

Ulatowski L., Ghelfi M., West R., Atkinson J., Finno C.J, & Manor D. (2022). The tocopherol transfer protein mediates vitamin E trafficking between cerebellar astrocytes and neurons. The Journal of Biological Chemistry, 298(3), 101712.

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