Ft400emt

Manufactured by Thorlabs

Sourced in United States

The FT400EMT is a fiber-coupled broadband light source from Thorlabs. It generates light over a wide spectral range and is designed for use in fiber optic applications. The device features a compact design and provides a stable output power.

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Lab products found in correlation

Pm100d, by Thorlabs (1 mentions) Ted200c, by Thorlabs (1 mentions) Ldc240c, by Thorlabs (1 mentions) Pm 100, by Thorlabs (1 mentions) Spike2, by Cambridge Electronic Design (1 mentions) T cube led driver, by Thorlabs (1 mentions) Axiovert 200 system, by Zeiss (1 mentions) Lf plus, by Ametek (1 mentions)

4 protocols using ft400emt

Laser-Powered NMR Spectroscopy Protocol

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The light source used for the experiments consisted of a 450 nm laser diode with a 1.6 W maximum output power. The optical power is decreased to the milliWatt range by using a controller that regulates the current through the diode. The current controller (Thorlabs, LDC240C) can be switched on and off to regulate the duration and intensity of the light manually. A temperature controller (Thorlabs, TED200C) is also used to ensure no variation of the output light power. A photometer PM100D (Thorlabs) was used to monitor the desired power from the light beam coming out of a 400 µm (internal diameter) optical fiber. An optical fiber (Thorlabs, FT400EMT) of 400 µm diameter is used to guide the light from the laser diode to the sample. All photo-CIDNP experiments were carried out in continuous light irradiation mode, in combination with continuous flow regime (except for the detection of 5-fluorouracile which was acquired in stopped-flow conditions). The enhancement factor experiments for Fig. 8 were acquired with a 600 µm (internal diameter, FT600EMT) optical fiber and optimal output powers were determined to obtain maximum signal (Supplementary Figs. 10–14).

Gomez M.V., Baas S, & Velders A.H. (2023). Multinuclear 1D and 2D NMR with 19F-Photo-CIDNP hyperpolarization in a microfluidic chip with untuned microcoil. Nature Communications, 14, 3885.

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Optical Stimulation of ChR2-Expressing S1 Cortex

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A 3-foot long, 400-μm diameter optic fiber (0.39NA, FT400EMT, Thorlabs, Newton, NJ USA) was coupled to a 473 nm laser source (AL-473-60TA, Aixiz, Huston, TX USA) through a customized collimation setup. The other end of the optic fiber was mounted on top of the center of the right S1 craniotomy (AP = −0.5 mm, ML = 3.7 mm) without touching the brain tissue. The power of the light at the end of the optical fiber was measured with a digital optical power meter (PM100, Thorlabs, Newton, NJ USA). Various light intensities at the fiber output were tested in control rats not expressing ChR2, and 63.66 mW mm⁻² was selected under which no significant cerebral blood flow and BOLD fMRI changes were observed in response to the light illumination. Light stimulation consisted of 20 ms of light pulses repeated at 20 Hz controlled by a shutter controller (SC10, Thorlabs, Newton, NJ USA) triggered via TTL signals which was generated and programmed using Spike 2 software (Cambridge Electronic Design. Cambridge, UK).

Li N., van Zijl P., Thakor N, & Pelled G. (2014). Study of the spatial correlation between neuronal activity and BOLD fMRI responses evoked by sensory and channelrhodopsin-2 stimulation in the rat somatosensory cortex. Journal of molecular neuroscience : MN, 53(4), 553-561.

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Optostimulation Cell Shape Analysis

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Zeiss Axiovert 200 system was used to observe cell shape changes with optostimulation. The T-Cube LED Driver (Thorlabs, Newton, NJ, LEDD1B) and Blue (470 nm) Fiber-Coupled High-Power LED (Thorlabs, Newton, NJ, M470F1) were used to generate optostimulation (~24 mW/mm²) with optical fiber (Thorlabs, Newton, NJ, FT400EMT). Cell images were recorded continuously with a 20 s interval. Additional cell contraction was achieved with a 5 min exposure to 20 mM KCl. Data were accepted for further analysis if the features of VSM cells were seen with YFP signal and KCl response. Cell shortening was measured along its longitudinal axis by the ImageJ 1.48 software.

Wu Y., Li S.S., Jin X., Cui N., Zhang S, & Jiang C. (2015). Optogenetic approach for functional assays of the cardiovascular system by light activation of the vascular smooth muscle. Vascular pharmacology, 71, 192-200.

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Thulium Fiber Laser Debonding with Temperature

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A 1940-nm Thulium Fiber Laser System (IPG Laser, TLR-5-1940, Germany) was used in CW mode. The output power of the laser was measured by an optical power meter (Newport, Model 1918-C) at the beginning of the each experiment. Output of the Tm:fiber laser was coupled with a silica optical fiber with 400-μm diameter (Thorlabs, FT400EMT) (Fig. 1). During laser application, the debonding force was measured with a modified universal testing machine (Ametek Lloyd Instruments, LF Plus, United Kingdom). Special gripping jaws and a testing frame were designed and implemented for placing the gypsum block properly. The testing machine was set to pull the bracket with a constant speed of 1 mm∕ min. During the debonding procedure, intrapulpal temperature changes were recorded by a K-type thermocouple system (OMEGA, OM-CP-0CTTTEMP, United Kingdom).

Demirkan I., Sarp A.S, & Gülsoy M. (2016). Ceramic bracket debonding with Tm:fiber laser. Journal of biomedical optics, 21(6).

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