The pUC19 plasmid and restriction enzymes were obtained from New England Biolabs (Ipswich, MA). The T4-kinase, T4 ligase and proteinase K were purchased from ThermoFisher Scientific; E. coli DNA gyrase was from Millipore Sigma. The 2′-oligodeoxynucleotides were purchased from Integrated DNA Technologies (Coralville, IA, USA) and purified by denaturing polyacrylamide gel electrophoresis.
Puc19 plasmid and restriction enzymes
Manufactured by New England Biolabs
The PUC19 plasmid is a commonly used cloning vector, and the restriction enzymes are molecular biology tools used to cleave DNA at specific sequences. The plasmid and enzymes can be used for various genetic engineering applications, though their specific intended uses are not provided here.
Automatically generated - may contain errors
2 protocols using puc19 plasmid and restriction enzymes
1
Plasmid Isolation and Manipulation
2
Cloning and Characterization of pUC19
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