The herb was purchased from the herb market (Guo Xin Pharmacy, Jiangsu, China) and identified as the root of Panax quinquefolius L. by Prof. Cunli Zhang of Northwest Agriculture and Forestry University of Science and Technology. We purchased 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2-NBDG) from Shanghai Dibai Biotechnology Co., Ltd. (Shanghai, China). N-Phenylthiourea (PTU), tricaine, alloxan, 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA), acridine orange (AO), riboflavin, catalase (CAT) activity assay kit and malondialdehyde (MDA) content assay kit were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).
2 7 dichlorodihydrofluorescein diacetate dcfh da
Manufactured by Solarbio
Sourced in China
2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA) is a non-fluorescent compound that can be oxidized to the highly fluorescent 2,7-dichlorodihydrofluorescein (DCF). The conversion of DCFH-DA to DCF is commonly used as an indicator of intracellular reactive oxygen species (ROS) levels.
Automatically generated - may contain errors
Lab products found in correlation
Mito tracker green, by Beyotime (2 mentions)
Malondialdehyde mda content assay kit, by Solarbio (1 mentions)
Acridine orange, by Solarbio (1 mentions)
Catalase cat activity assay kit, by Solarbio (1 mentions)
Penicillin streptomycin solution, by Solarbio (1 mentions)
Lsm 900, by Zeiss (1 mentions)
Dmem basic 1x, by Thermo Fisher Scientific (1 mentions)
Hoechest 33342, by Beyotime (1 mentions)
Cellular grade dmso, by Solarbio (1 mentions)
Lysotracker green, by Beyotime (1 mentions)
Hypoxia chamber, by STEMCELL (1 mentions)
Fitc anti mouse f4 80, by BioLegend (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Maclin Biochemical Technology (1 mentions)
Gelatin, by Maclin Biochemical Technology (1 mentions)
Cocl2 6h2o, by Maclin Biochemical Technology (1 mentions)
O phenylenediamine, by Maclin Biochemical Technology (1 mentions)
Lactate dehydrogenase ldh cytotoxicity assay kit, by Beyotime (1 mentions)
Paraformaldehyde (pfa), by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
C11 bodipy 581 591, by Cayman Chemical (1 mentions)
5 protocols using 2 7 dichlorodihydrofluorescein diacetate dcfh da
1
Antioxidant and Hypoglycemic Effects of Panax quinquefolius
2
Cytotoxicity Evaluation of Cells under Hypoxia
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Fetal bovine serum (FBS) was purchased from AusGeneX (Molendinar, Australia, CAT NO. FBS500-S); DMEM basic (1X) was from Gibco Life Technologies (Shanghai, China); penicillin/streptomycin solution (100 U/mL and 100 mg/mL, respectively), 2′, 7′-dichlorodihydrofluorescein diacetate (DCFH-DA), and Cellular grade DMSO were from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China); 3-(4,5-dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide(MTT) was obtained from Biosharp Life Technology Co., Ltd. (Hefei, China); Hoechest 33342, Mito-Tracker Green, and Lyso-Tracker Green were from Beyotime Biotechnology Co., Ltd. (Shanghai, China). All cells were cultured in a CO2 incubator (Wiggens, WCI-180, Beijing, China). All the in vitro fluorescence images experiments were obtained by using an Olympus IX73 + DP73 inverted microscope and laser confocal microscope (Carl Zeiss LSM 900, Jena, Germany). Hypoxic condition was created by coverslips (Citoglas, Hong Kong, China) and a hypoxia chamber (Stemcell Technologies, Cat 27310, Kent, WA, USA). Cytotoxicity assay was performed using an LED light (F&V, Z96kit, Fuijan, China) as irradiation source.
3
Multifunctional Antimicrobial Hydrogel Synthesis
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CoCl2·6H2O, tannin, silver acetate, 3,3',5,5'-tetramethylbenzidine (TMB), o-phenylenediamine (OPD) and gelatin were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). Acrylic anhydride was purchased from Sigma (St Louis, MO, USA). Hydrogen peroxide (H2O2, 30%), aqueous ammonia, acetic acid and ethanol were the products of Tianjin Kermel Chemical Plant (Tianjin, China). 2,2-Dimethoxy-2-phenylacetophenone (DMPA), the live/dead (SYTO 9/PI) bacterial viability kit and PE anti-mouse CD45 (Cat# 12-0451-82) were received from Thermo Fisher Scientific (New Jersey, USA). The lactate dehydrogenase (LDH) cytotoxicity assay kit was obtained from Beyotime (Shanghai, China). 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) was obtained from Solarbio Life Science (Beijing, China). FITC anti-mouse F4/80 (Cat# 123108) and FITC anti-mouse Ly-6C/Ly-6C (Gr-1) (Cat# 108406) were obtained from Biolegend (San Diego, CA, USA). Anti-Ly6G + Ly6c (ab25377) and anti F4/80 (ab111101) were purchased from Abcam (Cambridge, UK). GTVisionTM I detection system/Mo&Rb (GK500510A) was received from Gene Tech (Shanghai) company limited. Four strains of MDR P. aeruginosa (21027, 20059, 21173, 21715) were kindly provided by Qingdao Municipal Hospital (China). Doubly deionized water was purified by a Unique-R20 purification system (Xiamen, China).
4
Quantifying Cellular Oxidative Stress
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The cells were inoculated with 1 × 105 cells/well in 24-well plates. Then, 500 μL of medium containing different drug concentrations was added to the plates, incubated for 24 h, and washed twice with PBS. The solution contained 10 μM of 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA, Solarbio, Beijing, China) in a serum-free medium and was incubated for 30 min in the dark. The cells were washed in PBS three times. Next, 4% paraformaldehyde (Beyotime, Shanghai, China) was added to the system and the cells were fixed at room temperature for 10 min. After the cells had been washed in PBS three times, 4’,6-diamidino-2-phenylindole (DAPI, Solarbio, Beijing, China) was used for staining for 10 min, the cells were washed three times with PBS again, and the fluorescence intensity was observed using a fluorescence microscope and photographed. ImageJ/Fiji, an open-source image processing package, was used to analyze the mean fluorescence intensity.
5
Curcumin-Mediated Ferroptosis Regulation
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Curcumin (Cur) was purchased from Sigma-Aldrich. 5-aminolevulinic acid hydrochloride (ALA·HCl), iron (III) chloride (FeCl3), 1,10-phenanthroline, and methylene blue (MB) were purchased from Aladdin. 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Beijing Solarbio Science & Technology. Mito-tracker green, cell plasma membrane staining kit with DiD, and Ad-mCherry-GFP-LC3B were obtained from beyotime. FeRhoNox-1 (Fe2+ indicator) and hydroxyphenyl fluorescein (HPF) were purchased from Shanghai Maokang Biotechnology Co., Ltd. Ferrostatin-1 (Fer-1), biliverdin (BV), and bilirubin (BR) were obtained from Shanghai yuanye Bio-Technology Co., Ltd. BODIPY 581/591 C11 was purchased from Cayman Chemical Company. Fetal bovine serum (FBS) was obtained from PAN. Roswell Park Memorial Institute (RPMI) 1640 medium, penicillin-streptomycin, trypsin-EDTA, and PBS (1×, pH 7.4) were purchased from M&C gene Technology (Beijing) Ltd. Anti-Glutathione Peroxidase 4 antibody (ab125066), Anti-FACL4 antibody (ab227256) were purchased from Abcam.
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