Wh 200 400 mz spectrometer

Rat intestinal acetone powder was purchased from Sigma Aldrich, St. Louis (USA), Acarbose was purchased from Glucobay Bayer AG (Germany). Porcine pancreatic α-Amylase and Yeast α-Glucosidase were purchased from SRL, Mumbai (India). Glucose oxidase (GOD POD) kit was purchased from Piramal HealthCare Ltd, Mumbai (India). All other chemicals used were of analytical grade and purchased from Sigma Aldrich, St. Louis (USA) and SRL, Mumbai (India). All IR spectra were obtained in KBr disc on a Shimadzu FT-IR 157 Spectrometer. ¹H and ¹³C NMR spectra were recorded on a Bruker WH-200 (400 MZ) spectrometer in CDCl₃ or DMSO-d₆ as solvent, using TMS as an internal standard and chemical shifts are expressed as ppm. Mass spectra were determined on a Shimadzu LC-MS. The elemental analyses were carried out using an Elemental Vario Cube CHNS rapid Analyzer. The progress of the reaction was monitored by TLC pre-coated silica gel G plates.

The mixture of 3-bromopyridine-2-amine (200 mg, 0.08mmol), 3-bromopentane-2, 4-dione (142 mg, 0.08 mmol) and 4 mL of tetrahydrofuran (THF) were taken in a sealed tube and heated at 60°C for 4 h and the reaction was monitored by TLC. After the completion of reaction, boronic acids (0.08 mmol) were added along with Pd(dppf)Cl₂ (0.002 mmol) and K₂CO₃ (0.17 mmol). Finally, 1mL of water was added and the reaction was continued for 4 h at 60°C. Solvent was evaporated to obtain the crude product and further it was purified by passing through the column chromatography using hexane and ethyl acetate as solvents.
All IR spectra were obtained in KBr disc on a Shimadzu FT-IR 157 Spectrometer. ¹H and ¹³C NMR spectra were recorded on a Bruker WH-200 (400MZ) spectrometer in CDCl₃ or DMSO-d₆ as solvent, using TMS as an internal standard and chemical shifts are expressed as ppm. Mass spectra were determined using LC-MS. (Shimadzu). The elemental analyses were carried out using an Elemental Vario Cube CHNS rapid Analyser. The progress of the reaction was monitored by TLC pre-coated silica gel G plates. Melting points were determined in a melting point apparatus and were uncorrected. The structures of novel imidazopyridine derivatives are presented in Table 1. Spectra (S1 Data) and characterization data is provided as supplementary data (S2 Data).