IR spectra were obtained as solid or neat liquid on a Fourier Transform Bruker Vector 22 spectrometer. Only significant absorptions are listed. The 1H and 13C NMR spectra were recorded with Bruker Avance 300 (300 and 75 MHz, for 1H and 13C, respectively) or Bruker Avance 400 (400 and 100 MHz for 1H and 13C, respectively) spectrometers. Mass spectra were recorded with a Bruker Esquire-LC instrument. Elemental analyses were performed by the Microanalysis Service in ICSN–CNRS, Gif-Sur-Yvette — France. Analytical thin-layer chromatography was performed with Merck silica gel 60 F254 glass precoated plates (0.25 mm layer) and Merck aluminum oxide 60F254 neutral sheets. Column chromatography was performed with Merck silica gel 60 (230–400 mesh ASTM) and Fluka aluminum oxide type 507C neutral. All reactions involving air- or water-sensitive compounds were routinely conducted in oven- or flame-dried glassware under a positive pressure of nitrogen. Except as otherwise indicated, all reactions were carried out in distilled solvents. Triethylamine was distilled over calcium hydride. Chemicals obtained from commercial suppliers were used without further purification.
Esquire lc
Manufactured by Bruker
Sourced in Germany
The Esquire-LC is a liquid chromatography-mass spectrometry (LC-MS) system developed by Bruker. It is designed to provide high-performance liquid chromatography (HPLC) and mass spectrometry (MS) capabilities for analytical applications. The core function of the Esquire-LC is to separate, detect, and analyze chemical compounds in liquid samples.
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Lab products found in correlation
Avance 400, by Bruker (2 mentions)
Vector 22, by Bruker (2 mentions)
Avance 300, by Bruker (2 mentions)
Xevo g2 xs qtof mass spectrometer, by Waters Corporation (1 mentions)
Avance 3 600 mhz spectrometer, by Bruker (1 mentions)
Proteinlynx global server, by Waters Corporation (1 mentions)
Synapt g2 si, by Waters Corporation (1 mentions)
Amazon etd, by Bruker (1 mentions)
Amazon qit, by Bruker (1 mentions)
241 polarimeter, by PerkinElmer (1 mentions)
7 protocols using esquire lc
1
Spectroscopic Characterization of Organic Compounds
2
Spectroscopic Characterization of Organic Compounds
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Flash column chromatography (FCC) was performed using silica gel 200–300 mesh from Acros Organics occasionally on a Büchi Sepacore system equipped with a UV monitor. For thin layer chromatography (TLC), silica gel plates Merck 60 F254 were used. Spots detected on the TLC plates under the UV lamp at 254 or 365 nm were visualized by spraying Dragendorff’s reagent. The EI-MS were recorded on a Finnigan MAT-95 mass spectrometer (70 eV) and the ESI-MS on an Bruker Esquire LC with an ion trap detector. Positive and negative ions were detected. ESI-HRMS were recorded on a Waters Xevo G2-XS QTOF mass spectrometer. The 1D and 2D NMR spectra (600 MHz 1H NMR and 150 MHz 13C NMR) were measured on a Bruker AVANCE III 600 MHz spectrometer using CDCl3 or CD3OD as solvents. The chemical shifts δ are reported in ppm using either CHCl3/CDCl3 (δH = 7.25, δC = 77.0) or CH3OH/CD3OD (δH = 3.31, δC = 49.0 ppm) as internal standard relative to TMS. Coupling constants J are given in Hz. The complete assignment of 1H and 13C NMR signals was confirmed by 2D NMR spectroscopic methods (COSY, HSQC, HMBC and NOESY).
3
Mass Spectrometric Analysis of HDX Peptides
4
Quantification of 5-OH Coniferyl Alcohol Glycoside
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Soluble phenolics were extracted twice from 30.0 ± 0.05 mg of the lyophilized above‐ground stem samples with 1.0 mL 50% methanol plus 1.5% acetic acid for 3 h at room temperature each time (Fu et al., 2011b ). Identification and quantification of 5‐OH coniferyl alcohol glycoside was performed by LC‐PDA/ESI‐MS/MS according to a previously described method (Fu et al., 2011b ). All the mass spectra were acquired using a Bruker Esquire LC equipped with an electrospray ionization (ESI) source. Mass spectra from positive‐ and negative‐ion ESI were recorded over the range of 50–2200 m/z. To confirm the aglycone structure of the deduced compound, the vacuum‐dried methanolic extracts of switchgrass internodes were resolved with 3 mL of 5 mg/mL β‐glucosidase in citric acid buffer (pH = 5.5) and the reaction was incubated at 37 °C overnight (Tian and Dixon, 2006 ). The β‐glucosidase hydrolysis products were vacuum‐dried, re‐dissolved in 1.0 mL 80% methanol and were identified by comparing their retention time, and UV‐visible and mass spectra with the corresponding standard compounds. The reference standard of 5‐OH coniferyl alcohol was synthesized by the Chemistry Research Solution LLC (PA, USA).
5
Metastable Atom Dissociation Mass Spectrometry
6
Characterization of Organic Compounds
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Infrared (IR) spectra were obtained as solid or neat liquid on a Fourier Transform Bruker Vector 22 spectrometer. Only significant absorptions are listed. Optical rotations were measured on a Perkin-Elmer 241 Polarimeter at 589 nm. The 1H and 13C nuclear magnetic resonance (NMR) spectra were recorded on Bruker Avance 300 (300 MHz and 75 MHz for 1H and 13C, respectively) or Bruker Avance 400 (400 MHz and 100 MHz for 1H and 13C, respectively) spectrometers. Recognition of methyl, methylene, methine, and quaternary carbon nuclei in 13C NMR spectra rests on the J-modulated spin-echo sequence. Mass spectra were recorded on a Bruker Esquire-LC. High-resolution mass spectra were recorded on a electrospray ionization time-of-flight (ESI/TOF) (LCT, Waters, Milford, MA, USA) LC-spectrometer. Analytical thin-layer chromatography (TLC) was performed on Merck silica gel 60F254 glass precoated plates (0.25 mm layer). Column chromatography was performed on Merck silica gel 60 (230–400 mesh ASTM). Toluene, pyridine, dimethylformamide (DMF), and CH2Cl2 were distilled from calcium hydride, under a nitrogen atmosphere. All reactions involving air- or water-sensitive compounds were routinely conducted in glassware, which was flame-dried under a positive pressure of nitrogen.
7
Quadrupole Ion Trap Mass Spectrometry
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