Salvianolic acid b

According to the Pharmacopoeia of the People's Republic of China (2020 edition), six main components in BZXD have been identified. The quality stability and repeatability of these six marker components were detected by UPLC-MS/MS analysis method. We purchased the standard reference materials of Rosmarinic acid, Paeoniflorin, Glycyrrhizic acid, Salvianolic acid B, p-Coumaric acid, and Ferulic acid from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Qualitative analysis was carried out using an AB Sciex API 4000 LC/MS/MS system. Analytes were separated using a linear gradient program at a flow rate of 0.30 ml/min using a mobile phase consisting of 2 mmol/l ammonium acetate in water (A) and acetonitrile (B).
Precisely pipette 0.200 ml of thawed BZXD concentrate at 4 °C into a 2 ml brown PE tube, 0.6 ml mass spectrometry grade acetonitrile solution, and vortex for 5 min. Centrifuge at 13000r/min at 4 °C for 20 min. Take the supernatant and dilute it by 4 times, shake and mix, and filter through a 0.22-micron filter to be tested. The samples were detected and analyzed by negative ion electrospray tandem mass spectrometry (Supplementary Figure S1).

S. miltiorrhiza medicinal slices were purchased from the Anhui Renpu Pharmaceutical Co. (Anhui, China). The AB-8 macroporous adsorption resin (lot no. 312L011) was obtained from Beijing Solebo Technology Co. (Beijing, China). SAA, salvianolic acid B, and OSR (purity ≥ 98.0%) were purchased from Shanghai Yuanye Biotechnology (Shanghai, China). CCK8 was purchased from Beyotime (Shanghai, China). Hoechst 33342, propidium iodide (PI), and RNase were purchased from Keygen Biotech (Shanghai, China). Anti-CyclinB1 and anti-CDK1 antibodies were purchased from Boster Biotechnology (Shanghai, China). Analytical grade hydrochloric acid and other chemicals were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China).