Opti-MEM I reduced serum medium and G418 were purchased from Invitrogen (Carlsbad, CA, USA) and the X-tremeGENE HP reagent was purchased from Roche (Basel, Switzerland). Cell culture medium and fetal bovine serum were obtained from Hyclone (Beijing, China). Alternative Thioglycollate Medium, Pertussis toxin (PTX), GF109203X (bisindolylmaleimide), Go6983, and tyrphostin A9 were obtained from Sigma (St. Louis, MO, USA), while U0126, tyrphostin AG1478, GM6001, PP2 and Wortmannin were from Calbiochem (La Jolla, CA, USA). Anti-phospho-Akt (Ser473), Anti-phospho-Akt(Thr308), Anti–Akt, Anti–EGFR, Anti–PDGFR, Anti-phospho-EGFR (Tyr1173), Anti-phospho-PDGFR (Tyr1018) and the horseradish peroxidase substrate were bought from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase-conjugated goat anti-rabbit secondary antibody and anti-β-actin antibody were obtained from HuaAn Biotechnology (Hangzhou, China). RIPA lysis buffer and a BCA kit were bought from Beyotime (Haimen, China).
Anti β actin antibody
Manufactured by HuaAn Biotechnology
Sourced in China
The Anti-β-actin antibody is a primary antibody that specifically binds to the β-actin protein, a ubiquitous cytoskeletal protein found in eukaryotic cells. This antibody is commonly used in various research applications, such as Western blotting, immunocytochemistry, and immunohistochemistry, to detect and quantify the expression levels of β-actin.
Automatically generated - may contain errors
Lab products found in correlation
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca kit, by Beyotime (1 mentions)
X tremegene hp reagent, by Roche (1 mentions)
Anti egfr, by Cell Signaling Technology (1 mentions)
Anti phospho egfr tyr1173, by Cell Signaling Technology (1 mentions)
Tyrphostin a9, by Merck Group (1 mentions)
Quantity one analysis software, by Bio-Rad (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Anti nf κb p65, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions)
Anti phospho p65, by Cell Signaling Technology (1 mentions)
3 protocols using anti β actin antibody
1
Cell Signaling Pathway Reagents Comparison
2
Protein Expression Analysis in Rat Brain
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The right cerebral cortex was obtained to extract total protein. Protein was quantified using the bicinchoninic acid method. A total of 50 μg protein was loaded on the gel for 2 hours of electrophoresis and then transferred to 0.45 μm polyvinylidene difluoride membranes at a constant current for 2 hours. The membrane was blocked with 5% milk for 2 hours, incubated with primary rabbit anti-β-actin antibody (1:5,000; HangZhou HuaAn Biotechnology Co., Ltd., Hangzhou, China), rabbit anti-rat DMT1-with IRE (1:3,000; Alpha Diagnostics, Owings Mills, MD, USA), rabbit anti-rat DMT1-without IRE (1:2,000; Alpha Diagnostics) and rabbit anti-mouse FPN1 (1:3,000; Alpha Diagnostics) at 4°C overnight, washed three times with Tris-buffered saline and Tween, each for 5 minutes, incubated with secondary antibody goat anti-rabbit (1:10,000; KPL, Gaithersburg, MD, USA) at room temperature for 1 hour, and washed three times with 0.05% Tris-buffered saline and Tween. DMT1-with IRE, DMT1-without IRE and FPN1 protein expressions were detected using a photochemical chromogenic method. β-Actin was used as an internal reference. The optical density of protein bands was analyzed using Quantity One analysis software (Bio-Rad Laboratories). The protein expression was expressed as the optical density ratio of target protein to β-actin.
3
TNFAIP3 Regulation by miR-19b-3p
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The anti-TNFAIP3 antibody was purchased from Arigo Biolaboratories (Hamburg, Germany). Anti-β-actin antibody was from HuaAn Biotechnology Co., Ltd. (Hangzhou, China). Anti-NF-κB p65, anti-phospho-p65, HRP-conjugated anti-rabbit IgG, and anti-mouse IgG were obtained from Cell Signaling Technology (Danvers, MA, USA). Transfection reagent LipofectamineTM 3000 was purchased from Invitrogen (Carlsbad, CA, USA). The all-in-one CRISPR/Cas9 plasmid pYSY-spCas9-sgRNA-Puro for gene editing was purchased from YSY Biotech Co. LTD. (Nanjing, China). RNA oligonucleotides, including miR-19b-3p mimics (double-stranded RNA oligonucleotides), inhibitors (single-stranded chemically modified oligonucleotides), and their respective control oligonucleotides, were commercially available at GenePharma (Shanghai, China). Sequences of all oligonucleotides are listed in Table 1 .
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