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Ht 7700 thermocycler

Manufactured by Thermo Fisher Scientific
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Sourced in Japan

The HT-7700 thermocycler is a compact and efficient instrument designed for PCR amplification of DNA samples. It features a temperature range of 4°C to 99°C, and can precisely control the temperature of the samples during the thermal cycling process. The HT-7700 has a block capacity of 96 wells and is capable of performing a wide range of PCR applications.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Iscript cdna synthesis kit, by Bio-Rad (2 mentions) Sybr green kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Thermocycler (175 citations) HT-7700 (3 citations)

2 protocols using ht 7700 thermocycler

1

Quantifying Angiogenic Factors Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
A portion of the harvested cells was prepared for RT-PCR analysis. The RNA was extracted using an RNeasy Mini Kit (Qiagen, Tokyo, Japan), and subsequently reverse-transcribed using an iScript cDNA Synthesis Kit (Bio-Rad, Tokyo, Japan), to generate cDNA. The PCR reaction and analysis were carried out using an HT-7700 thermocycler (Life Technologies, Tokyo, Japan) with the SYBR-green kit (Life Technologies, Tokyo, Japan). Primers were designed to detect vascular endothelial growth factor (VEGF), angiopoietin-like 2 (ANGPTL2), and insulin-like growth factor-1 (IGF-1). The relative abundance of each target gene was determined by normalizing the gene-specific mRNA levels to that of an internal control (GAPDH).
Li C., Matsushita S., Li Z., Guan J, & Amano A. (2017). c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy. Journal of stem cell research & therapy, 7(10), 402.
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2

Quantifying Angiogenic Factors Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
A portion of the harvested cells was prepared for RT-PCR analysis. The RNA was extracted using an RNeasy Mini Kit (Qiagen, Tokyo, Japan), and subsequently reverse-transcribed using an iScript cDNA Synthesis Kit (Bio-Rad, Tokyo, Japan), to generate cDNA. The PCR reaction and analysis were carried out using an HT-7700 thermocycler (Life Technologies, Tokyo, Japan) with the SYBR-green kit (Life Technologies, Tokyo, Japan). Primers were designed to detect vascular endothelial growth factor (VEGF), angiopoietin-like 2 (ANGPTL2), and insulin-like growth factor-1 (IGF-1). The relative abundance of each target gene was determined by normalizing the gene-specific mRNA levels to that of an internal control (GAPDH).
Li C., Matsushita S., Li Z., Guan J, & Amano A. (2017). c-kit Positive Cardiac Outgrowth Cells Demonstrate Better Ability for Cardiac Recovery Against Ischemic Myopathy. Journal of stem cell research & therapy, 7(10), 402.
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