Anti human cd8 sk1

Manufactured by BioLegend

Anti-human CD8 (SK1) is a monoclonal antibody that specifically binds to the CD8 antigen expressed on the surface of human T cells. CD8 is a co-receptor for the T cell receptor and plays a role in the activation and differentiation of cytotoxic T cells.

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Lab products found in correlation

Anti mouse cd45 clone 30 f11, by BD (2 mentions) Anti mouse cd4 clone gk1, by BD (2 mentions) Anti human cd3 sk7, by BioLegend (2 mentions) Anti human cd4 sk3, by BioLegend (2 mentions) Anti mouse cd8a clone 53 6, by BD (2 mentions) Anti mouse f4 80 clone bm8, by BioLegend (1 mentions) Anti mouse ifn γ xmg1, by Thermo Fisher Scientific (1 mentions) Rabbit anti ifngr1, by Merck Group (1 mentions) Anti mouse cd3 clone 17a2, by BioLegend (1 mentions) Anti mouse b220 clone ra3 6b2, by BioLegend (1 mentions) Anti mouse mhcii clone m5 114, by BioLegend (1 mentions) Anti mouse cd11b m1 70, by Thermo Fisher Scientific (1 mentions) Antimouse gr1 clone rb6 8c5, by BioLegend (1 mentions) Anti mouse foxp3 clone mf23, by BD (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Anti mouse cd28, by BioLegend (1 mentions) Clone 37, by BioLegend (1 mentions) Bovine serum albumin (bsa), by GE Healthcare (1 mentions) Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-CD8 (141 citations) Anti-human CD8 (9 citations) CD8 (SK1, (7 citations) Anti-CD8 (SK1, (4 citations)

3 protocols using anti human cd8 sk1

Immunoblotting and Flow Cytometry Protocols

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For immunoblotting, these antibodies are used: rabbit anti-STAT1 (CST, #14994), rabbit anti–phospho-STAT1 (CST, #7649), rabbit anti-EZH2 (CST, #5246), mouse anti–MHC-I class I (Santa Cruz Biotechnology, #sc-55582), rabbit anti-IFNGR1 (Millipore, #MABF753), and mouse anti–β-actin (Sigma-Aldrich, #A5441).
For flow cytometry, the following fluorochrome-conjugated antibodies are used: anti-mouse H-2K^b/H-2D^b (clone 28-8-6, BioLegend), anti-mouse H-2Kb bound to SIINFEKL (clone 25-D1.16, BioLegend), anti-mouse CD3 (clone 17A2, BioLegend), anti-mouse CD28 (clone 37.51, BioLegend), anti-mouse granzyme B (clone GB11, BioLegend), anti-mouse B220 (clone RA3-6B2, BioLegend), anti-mouse CD49b (clone DX5, BioLegend), anti-mouse Gr-1 (clone RB6-8C5, BioLegend), anti-mouse MHC-II (clone M5/114.15.2, BioLegend), anti-mouse F4/80 (clone BM8, BioLegend), anti-mouse IFN-γ (XMG1.2, eBioscience), anti-mouse CD11b (M1/70, eBioscience), anti-mouse NK1.1 (clone PK136, BD), anti-mouse CD103 (clone M290, BD), anti-mouse CD206 (clone MR5D3, BD), anti-mouse CD24 (clone M1/69, BD), anti-mouse CD4 (clone GK1.5, BD), anti-mouse CD45 (clone 30-F11, BD), anti-mouse CD8a (clone 53-6.7, BD), anti-mouse Foxp3 (clone MF23, BD), anti-mouse γδ TCR (clone GL3, BD), anti-human CD3 (SK7, BioLegend), anti-human CD4 (SK3, BioLegend), anti-human CD8 (SK1, BioLegend), and anti-human 137(4-1BB) (4B4-1, BioLegend).

Guo W., Wang Y., Yang M., Wang Z., Wang Y., Chaurasia S., Wu Z., Zhang M., Yadav G.S., Rathod S., Concha-Benavente F., Fernandez C., Li S., Xie W., Ferris R.L., Kammula U.S., Lu B, & Yang D. (2021). LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance. Science Advances, 7(7), eabb3555.

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Multiparametric Flow Cytometry Analysis

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Live immune cells from peripheral blood, spleen, and BM were determined by staining with live/dead fixable blue dead cell stain kit (Life Technologies) for 30 min prior to cell-specific marker labeling. Cells were labeled with anti-human CD34 (clone 581; BD Biosciences), anti-human CD3 (UCHT1; Biolegend), anti-human CD56 (MEM-188, Biolegend), anti-human CD14 (63D3; Biolegend), anti-human CD19 (SJ25C1; BD Biosciences), anti-human CD117 (104D2; Biolegend), anti-human CD38 (HB-7; Biolegend), anti-human CD33 (WM53; BD Biosciences), mouse CD45.1 (A20; BD Biosciences), anti-human CD8 (SK1; Biolegend), anti-human CD4 (SK3; BD Biosciences), and anti-human CD45 (HI30; Biolegend) monoclonal antibodies for 30 min at room temperature. After incubation, cells were washed and resuspended in FACs buffer containing phosphate buffered saline (PBS), 0.2% bovine serum albumin (GE Healthcare Life Sciences), and 0.05% sodium azide (Merck) for flow cytometry data acquisition. Data was acquired using a LSR II flow cytometer (BD Biosciences) with FACSDiva software, and analysis was performed using FlowJo software (version 10; Tree Star Inc). Absolute count of cells in peripheral blood was determined using CountBright™ Absolute Counting Beads (Thermo Fisher Scientific).

Her Z., Yong K.S., Paramasivam K., Tan W.W., Chan X.Y., Tan S.Y., Liu M., Fan Y., Linn Y.C., Hui K.M., Surana U, & Chen Q. (2017). An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia. Journal of Hematology & Oncology, 10, 162.

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Mouse Tumor Cell and PBMC Culture

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Mouse Luise Lung carcinoma (LLC) and B16 cell line were a kind gift from B. Lu’s laboratory, were cultured in DMEM high glucose (Hyclone) with 10 percent heal inactivated FBS (Gibco), 2 mM L-glutamine (Invitrogen, Carlsbad, CA), 50 U/penicillin (Invitrogen), streptomycin (50 μg/ml) (Invitrogen). Peripheral blood mononuclear cells (PBMCs) transduced with TCR gp100 and 526-MEL were obtained from Dr. U. Kammula’s laboratory. PBMCs were cultured in complete medium [RPMI-1640, 10% heat-inactivated human AB serum (Gemini Bio-Products, Woodland, CA), 2 mM L-glutamine (Invitrogen, Carlsbad, CA), 50 U/penicillin (Invitrogen), streptomycin (50 μg/ml) (Invitrogen), gentamicin (50 μg/ml) (Invitrogen), 10 mM HEPESs (Invitrogen), and Amphotericin B (250 ng/ml) (Invitrogen). Six-week female C57BL/6j mice and nude mice were purchased from Jackson laboratory. anti-mouse CD45 (clone 30-F11, BD), anti-mouse CD8a (clone 53-6.7, BD), anti-mouse CD4 (clone GK1.5, BD) CD69 (clone H1.2F3, Biolegend), anti-human CD3 (SK7, BioLegend), anti-human CD4 (SK3, BioLegend), anti-human CD8 (SK1, BioLegend), and anti-human 137(4-1BB) (4B4-1, BioLegend).

Bhuniya A., Pattarayan D, & Yang D. (2022). Lentiviral vector transduction provides a nonspecific immunogenicity for syngeneic tumor models. Molecular carcinogenesis, 61(12), 1073-1081.

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