Mouse cytokine 32 plex kit

Manufactured by Merck Group

The Mouse Cytokine 32-plex kit is a multiplex assay designed to simultaneously detect and quantify 32 different cytokines and chemokines in mouse biological samples. The kit utilizes Luminex technology to provide a comprehensive profile of the mouse cytokine and chemokine environment.

Automatically generated - may contain errors

Lab products found in correlation

Milliplex analyst software, by Merck Group (2 mentions)

Close spelling variants of this product

Mouse 32-Plex Cytokine Panel 1 kit MILLIPLEX Mouse Cytokine/Chemokine 32-plex kit

2 protocols using mouse cytokine 32 plex kit

Multiplexed Cytokine Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luminex assays were carried out using the FlexMAP 3D multiplexing platform (Luminex xMAP system). The Millipore Mouse Cytokine 32-plex kit was used to measure the serum concentration of 32 cytokines. xPONENT (Luminex) and Milliplex Analyst Software (Millipore) was applied to convert mean fluorescent intensities (MFI) values into molecular concentrations by the use of a standard curve (5-parameter logistic fitting method). Data were normalized by Z score transformation using the scale() function in R and visualized with the heatmap.2 function of the gplots package.

Kleppe M., Koche R., Zou L., van Galen P., Hill C., Dong L., De Groote S., Papalexi E., Hanasoge Somasundara A.V., Cordner K., Keller M., Farnoud N., Medina J., McGovern E., Reyes J., Roberts J., Witkins M., Rapaport F., Teruya-Feldstein J., Qi J., Rampal R., Bernstein B.E., Bradner J.E, & Levine R.L. (2017). Dual Targeting of Oncogenic Activation and Inflammatory Signaling Increases Therapeutic Efficacy in Myeloproliferative Neoplasms. Cancer cell, 33(1), 29-43.e7.

+ Open protocol

+ Expand

Multiplex Cytokine Profiling in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Luminex assays were carried out using the FlexMAP 3D multiplexing platform (Luminex xMAP system). Plasma levels of 32 cytokines were measured simultaneously using the Millipore Mouse Cytokine 32-plex kit. Serum samples were prepared following manufactures instructions. xPONENT (Luminex) and Milliplex Analyst Software (Millipore) was applied to convert mean fluorescent intensities (MFI) values into molecular concentrations by the use of a standard curve (5-parameter logistic fitting method).

Kleppe M., Kwak M., Koppikar P., Riester M., Keller M., Bastian L., Hricik T., Bhagwat N., McKenney A.S., Papalexi E., Abdel-Wahab O., Rampal R., Marubayashi S., Chen J.J., Romanet V., Fridman J.S., Bromberg J., Teruya-Feldstein J., Murakami M., Radimerski T., Michor F., Fan R, & Levine R.L. (2015). JAK-STAT Pathway Activation in Malignant and Non-Malignant Cells Contributes to MPN Pathogenesis and Therapeutic Response. Cancer discovery, 5(3), 316-331.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!