Vacutainer tubes with k2edta

As a pilot study, a total of 1000 blood samples (650 blood donors and 350 recipient patients) were selected for comparison of ABO and Rh blood grouping by the CTT and by the automated microplate LYRA system. The samples of the donors were collected at the site of blood donation. The samples of the patients that were collected in clinical wards and sent to the blood bank for blood grouping were included in this study. All the samples were collected in BD Vacutainer^® Tubes with K2EDTA and tested on the same day of collection. Samples adequate in the quantity required for testing by both the techniques were included in the study. Hemolyzed and/orlipemic samples were excluded from the study. Samples of neonates and infants up to 6 months of age were also excluded in view of the incomplete development of ABO antibodies in their plasma.
The samples were selected by simple random sampling by lottery method.

A total of 269 samples received in the blood bank from multitransfused patients and multigravida females during the study period were compared for 927 crossmatches. This selection aimed at including the samples with antibodies so that the concordance of the two techniques and the strength of reaction could be compared for incompatible crossmatch results. The samples were collected in BD Vacutainer^® Tubes with K2EDTA. Samples adequate in the quantity required for testing by both the techniques were included in the study. Samples that were hemolyzed and/or lipemic were excluded from the study.