The reduction of anti-CD20 antibody (Rituximab, produced by Teruisi Pharmaceuticals, Huzhou, China) for conjugation purposes was performed as previously described (19 (link), 20 (link)). Briefly, the antibody was added into sodium phosphate buffer, followed by mixing with Tris(2-chloroethyl) phosphate (Sigma). MMAE linked with valine–citrulline linker was added slowly to the pre-chilled and reduced antibody solution for conjugation reaction. The drug antibody ratio (DAR) was determined by hydrophobic interaction chromatography. The purity of Rituximab-MMAE was above 95%, the average DAR of Rituximab-MMAE was 4.2 ± 0.5, and the content of endotoxin was below 0.3 EU/mg. The structure and chemical characteristic of ADC can be found in Figures S1 and S2 in Supplementary Material.
Tris 2 chloroethyl phosphate
Manufactured by Merck Group
Sourced in Germany
Tris(2-chloroethyl) phosphate is a chemical compound used as a flame retardant additive in various materials. It is a clear, viscous liquid with a high boiling point. The compound functions by inhibiting the combustion process and improving the fire resistance of the materials it is added to.
Automatically generated - may contain errors
Lab products found in correlation
Tributyl phosphate, by Merck Group (2 mentions)
Galaxolide, by Merck Group (2 mentions)
Triphenyl phosphate, by Merck Group (2 mentions)
Dihydrocarbamazepine, by Merck Group (2 mentions)
Atrazine d5, by Merck Group (2 mentions)
Suprasolv hexane, by Merck Group (2 mentions)
Analytical grade hydrogen chloride, by ITW Reagents (2 mentions)
Mecoprop d3, by Merck Group (2 mentions)
Tonalide d3, by Merck Group (2 mentions)
Caffeine, by Merck Group (2 mentions)
Ibuprofen, by Merck Group (2 mentions)
Carbamazepine, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Deoxycholate, by Merck Group (1 mentions)
Magnetic bead, by Thermo Fisher Scientific (1 mentions)
4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (1 mentions)
Acidic tyrode s solution, by Merck Group (1 mentions)
Np 40, by Merck Group (1 mentions)
Ms grade trypsin, by Thermo Fisher Scientific (1 mentions)
4 protocols using tris 2 chloroethyl phosphate
1
Rituximab Conjugation Optimization
2
Proteomic Analysis of Mouse Embryo Development
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Pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) were obtained from San-Sheng Pharmaceutical Co., Ltd. (Shenyang, China). 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), glycerol, Triton X-100, Tween 20, NP-40, deoxycholate, ethylene diamine tetraacetic acid (EDTA), NaCl, tris(2-chloroethyl)phosphate (TCEP), chloroacetamide (CAA), ammonium bicarbonate (ABC), trifluoroacetic acid (TFA), K+ Simplex Optimised Medium (KSOM), M2 medium, hyaluronidase, and acidic Tyrode's solution were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium dodecyl sulfate (SDS), formate, acetonitrile (ACN), magnetic beads, MS-grade trypsin, TMTsixplex™ reagents, and TMTpro™ 16plex reagent were obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Phosphate buffer saline (PBS) was obtained from Solarbio (Beijing, China). Ethanol (EtOH) was obtained from Sinopharm Chemical Reagent (Beijing, China).
3
Analytical Standards for Environmental Contaminants
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Gas chromatography (GC) grade (Suprasolv) hexane, methanol, and ethyl acetate were obtained from Merck (Darmstadt, Germany). Analytical-grade hydrogen chloride was obtained from Panreac (Barcelona, Spain). Caffeine, acetaminophen, ibuprofen, methyl dihydrojasmonate, oxybenzone, ketoprofen, hydrocinnamic acid, 5-methylbenzotriazole, naproxen, carbamazepine, galaxolide, benzothiazole, diclofenac, methylparaben, benzotriazole, tonalide, OH-benzothiazole, tributyl phosphate, tris(2-chloroethyl) phosphate, triphenyl phosphate, triclosan, cashmeran, octylphenol, diazinon, celestolide, atrazine, bisphenol A, 2,4-D, atrazine D5, mecoprop D3, tonalide D3 and dihydrocarbamazepine were purchased from Sigma-Aldrich (Steinheim, Germany).
Trimethylsulfonium hydroxide (TMSH) was obtained from Fluka (Buchs, Switzerland). Strata-X 100 polymeric SPE cartridges (200 mg) were purchased from Phenomenex (Torrance, CA, USA) and 101 the 0.7 μm glass fibre filters (ø 47 mm) were obtained from Whatman (Maidstone, UK). 102 103
Trimethylsulfonium hydroxide (TMSH) was obtained from Fluka (Buchs, Switzerland). Strata-X 100 polymeric SPE cartridges (200 mg) were purchased from Phenomenex (Torrance, CA, USA) and 101 the 0.7 μm glass fibre filters (ø 47 mm) were obtained from Whatman (Maidstone, UK). 102 103
4
GC Analysis of Environmental Contaminants
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Gas chromatography (GC) grade (Suprasolv) hexane, methanol, and ethyl acetate were obtained from Merck (Darmstadt, Germany). Analytical-grade hydrogen chloride was obtained from Panreac (Barcelona, Spain). Caffeine, ibuprofen (racemic composition), carbamazepine, galaxolide, tributyl phosphate, tris(2-chloroethyl) phosphate, triphenyl phosphate, 4-octylphenol, atrazine D5, mecoprop D3, tonalide D3 and dihydrocarbamazepine were purchased from Sigma-Aldrich (Steinheim, Germany). Trimethylsulfonium hydroxide (TMSH) was obtained from Fluka (Buchs, Switzerland). Strata-X polymeric SPE cartridges (200 mg) were purchased from Phenomenex (Torrance, CA, USA) and the 0.7 µm glass fibre filters (ø 47 mm) were obtained from Whatman (Maidstone, UK).
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