Nunc microwell 96 well optical bottom black chimney plates

For oxidative stress measurements, cells were seeded at 10,000 cells per well and allowed to attach for 24 h prior to any experiments. We used Thermo Scientific™ Nunc™MicroWell™ 96‐Well Optical‐bottom black chimney plates with polymer base for all fluorescent stains. After staining with each fluorescent stain (below) on a separate plate, cells were imaged using a Tecan infinite measure m200 fluorescent plate reader. Due to differing growth rates of each cell line, cells were counted after each experiment using a Countess II FL cell counter and data was normalized to a total of 20,000 cells per well.

For oxidative stress measurements, cells were seeded at 10,000 cells per well and allowed to attach for 24 h prior to any experiments. We used Thermo Scientific™ Nunc™ MicroWell™ 96‐Well Optical‐bottom black chimney plates with polymer base treated with ply‐d‐lysine for all fluorescent stains. After staining with each fluorescent stain (below) on a separate plate, cells were imaged using a Tecan infinite measure m200 fluorescent plate reader. Due to differing growth rates of each cell line, cells were counted after each experiment using a Countess II FL cell counter and data were normalized to a total of 20,000 cells per well.